Loic Le Dean
IFREMER
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Featured researches published by Loic Le Dean.
Aquatic Living Resources | 2003
Gaël Bougaran; Loic Le Dean; Ewa Lukomska; Raymond Kaas; Régis Baron
Starting microalgae continuous culture generally includes a preliminary batch culture to obtain sufficient cell density. It is possible to apply continuous regime from the beginning of the culture (IC mode) rather than to begin by an initial batch (IB mode). It is our purpose to check that both initial modes lead to identical steady-state cell characteristics. The microalga Isochrysis galbana affinis Tahiti was used for this comparative study. With an initial cell density o f5◊1 0 5 cell ml ‐1 and a dilution rate of about 1.0 d ‐1 , both IB and IC modes led to identical cell density once steady-state is reached between 6 and 8 d after inoculation in the two cases. Cell concentration of chlorophyll a and pheopigment a were found to be similar for IB and IC modes at steady-state. Initial culture conditions did not influence saturation irradiance and oxygen consumption rate, which were found to be 650 ± 143 µmol phot m ‐2 s ‐1 and 1.54 ◊ 10 ‐3 ±1 0 ‐5 µmol O2 ml ‐1 min ‐1 , respectively. At steady-state, nutrient cell uptakes were qN = 83.3 ± 2.0 fmol N cell ‐1 d ‐1 for NO3 ‐ and qP = 5.5 ± 0.4 fmol P cell ‐1 d ‐1 for H2PO4 ‐ and did not exhibit significant differences between IB and IC modes. Under the prevailing experimental conditions, results show that IC mode involved very similar steady-state cell characteristics when compared to IB mode subsequent steady-state. IC mode could be an attractive alternative especially for experimental laboratory studies, as it should lead to higher flexibility in starting continuous cultures.
Ophelia | 1998
Myriam Morelli; Loic Le Dean; Vincent Vonau; Alain Diter
Abstract A cytogenetic study was carried out in the shrimp Penaeus indicus using semi-automatic image analysis, a technique for chromosome morphometric measurements newly applied in crustaceans. The chromosomal analysis of nauplii revealed a modal diploid chromosome number of 2n = 88, confirmed by the modal haploid chromosome number of n = 44 found in adult testis. The chromosome pairing, automatically performed by a software application, revealed variations in the karyotypic formula, not only due to differences of chromosome condensation between metaphases. In eight metaphases karyotyped were counted 27 to 35 metacentric (M), 8 to 15 submetacentric (SM), and 1 to 4 subtelocentric (ST) pairs. No telocentric chromosome or secondary constrictions were observed. No sex chromosomes could be identified. The karyotypic formula proposed for P. indicus was 27 M + 13 SM + 4 ST.
Aquaculture | 2012
Julie Marchetti; Gaël Bougaran; Loic Le Dean; Cyril Megrier; Ewa Lukomska; Raymond Kaas; Erell Olivo; Régis Baron; Rene Robert; Jean-Paul Cadoret
Marine Pollution Bulletin | 1993
Gilles Bocquene; François Galgani; Thierry Burgeot; Loic Le Dean; Phillipe Truquet
Archive | 2004
Rene Robert; Marie-Josèphe Chrétiennot-Dinet; Raymond Kaas; Véronique Martin-Jézéquel; Jeanne Moal; Jean-Rene Le Coz; Jean-Louis Nicolas; Eudes Bernard; Jean-Paul Connan; Loic Le Dean; Gaetane Le Gourrierec; Bertrand Leroy; Claudie Quéré
Aquatic Living Resources | 1988
Patrick Lassus; Jean-Paul Berthome; Pierre Maggi; Philippe Truquet; Loic Le Dean
Aquatic Living Resources | 1990
Patrick Lassus; Frédéric Proniewski; Cyrille Pigeon; Laurent Veret; Loic Le Dean; Philippe Truquet
Aquatic Living Resources | 1996
Philippe Truquet; Patrick Lassus; Giorgio Honsell; Loic Le Dean
Marine Ecology Progress Series | 1993
Thierry Burgeot; Gilles Bocquene; Philippe Truquet; Loic Le Dean; Jean-Charles Poulard; Didier Dorel; Arnauld Souplet; François Galgani
Algal Research-Biomass Biofuels and Bioproducts | 2018
Quentin Béchet; Noémie Coulombier; Christophe Vasseur; Thomas Lasserre; Loic Le Dean; Olivier Bernard