Lorea Bagazgoitia

Oncogenic H-Ras and PI3K signaling can inhibit E-cadherin-dependent apoptosis and promote cell survival after photodynamic therapy in mouse keratinocytes

Maintenance of E‐cadherin mediated cell–cell contacts is often required for the survival of epithelial cells and tissues. Here we report that oncogenic activation of H‐Ras in murine keratinocytes can prevent cell death induced by immunological disruption of E‐cadherin adhesion. A similar situation was observed in cells showing constitutive activation of the p110α catalytic subunit of class IA PI3K. This protective effect is associated with β‐catenin‐dependent transcription and with activation of survival factor Akt/PKB. In addition, we induced cell death by employing photodynamic therapy, using Zn‐phthalocyanine as a photosensitizer that targets E‐cadherin adhesion complexes. We have found that cell death based on this photodynamic action is also bypassed in cells showing constitutive activation of H‐Ras and p110α. Taken together, these results indicate that H‐Ras/PI3K/Akt signaling plays a key role in cell survival mediated by E‐cadherin cell–cell contacts. J. Cell. Physiol. 219: 84–93, 2009.

Expression of p53 and p16 in actinic keratosis, bowenoid actinic keratosis and Bowen's disease.

Introduction  Bowens disease (BD) and bowenoid actinic keratosis (bAK) have traditionally been differentiated according to the presence or absence of dysplasia in the follicular epithelium. p16 has been suggested to be a useful tool to make the differential diagnosis between BD and AK and as a marker of bad prognosis.

Venous malformation of the eyelid treated with pulsed-dye-1064-nm neodymium yttrium aluminum garnet sequential laser: an effective and safe treatment.

Venous malformations of the skin and subcutaneous tissue are compressible, blue-purple tumors that are present at birth. According to the location and symptoms caused, venous malformations can be treated with surgery, sclerotherapy, or a combination of both. Laser therapy can also be used, especially when surgery is contraindicated. We report the case of a 24-year-old man who presented with a venous malformation on the upper and lower left eyelids, which provoked a mechanical ptosis. Treatment with sequential pulsed-dye neodymium yttrium aluminum garnet (PDL-Nd:YAG) laser was performed. After 2 treatments, a marked reduction of volume and blanching of the venous malformation was observed, with satisfactory cosmetic results. The sequential PDL-Nd:YAG laser seems to be an effective and safe therapy for the treatment of cutaneous venous malformations. It penetrates deeper than pulsed-dye laser alone, and because it allows the use of lower fluencies than Nd:YAG laser alone, it reduces the risk of adverse effects.

Recientes avances en láser y otras tecnologías

Laser technology and other energy sources are rapidly finding a place in dermatology clinics. In the field of skin rejuvenation by fractional photothermolysis, although few controlled studies have been undertaken, several devices have emerged in recent years that appear less effective than laser ablation techniques but that are safer. The aim of this short article is to provide an introduction, though not treat in depth, the different emerging technologies in dermatology. We will focus particularly on lasers and light sources in improving applications such as the treatment of vascular lesions, acne, and encapsulated ink tattoos; light-emitting diodes; developments in the treatment of cellulitis; photodynamic therapy; suction methods; scarring; and finally the recent and doubtlessly future introduction of home devices for use in a range of dermatologic applications (depilation, rejuvenation, treatment of acne, etc).

In situ production of ROS in the skin by photodynamic therapy as a powerful tool in clinical dermatology.

Photodynamic therapy (PDT) is a clinical modality of photochemotherapy based on the accumulation of a photosensitizer in target cells and subsequent irradiation of the tissue with light of adequate wavelength promoting reactive oxygen species (ROS) formation and cell death. PDT is used in several medical specialties as an organ-specific therapy for different entities. In this review we focus on the current dermatological procedure of PDT. In the most widely used PDT protocol in dermatology, ROS production occurs by accumulation of the endogenous photosensitizer protoporphyrin IX after treatment with the metabolic precursors 5-methylaminolevulinic acid (MAL) or 5-aminolevulinic acid (ALA). To date, current approved dermatological indications of PDT include actinic keratoses (AK), basal cell carcinoma (BCC) and in situ squamous cell carcinoma (SCC) also known as Bowen disease (BD). With regards to AKs, PDT can also treat the cancerization field carrying an oncogenic risk. In addition, an increasing number of pathologies, such as other skin cancers, infectious, inflammatory or pilosebaceous diseases are being considered as potentially treatable entities with PDT. Besides the known therapeutic properties of PDT, there is a modality used for skin rejuvenation and aesthetic purposes defined as photodynamic photorejuvenation. This technique enables the remodelling of collagen, which in turn prevents and treats photoaging stygmata. Finally we explore a new potential treatment field for PDT determined by the activation of follicular bulge stem cells caused by in situ ROS formation.

Acute hyperkeratotic and desquamative reaction in a patient with Sézary syndrome treated with bexarotene.

© 2007 The Authors 389 JEADV 2008, 22, 363–404 Journal compilation

Recent Advances in Laser Therapy and Other Technologies

Laser technology and other energy sources are rapidly finding a place in dermatology clinics. In the field of skin rejuvenation by fractional photothermolysis, although few controlled studies have been undertaken, several devices have emerged in recent years that appear less effective than laser ablation techniques but that are safer. The aim of this short article is to provide an introduction, though not treat in depth, the different emerging technologies in dermatology. We will focus particularly on lasers and light sources in improving applications such as the treatment of vascular lesions, acne, and encapsulated ink tattoos; light-emitting diodes; developments in the treatment of cellulitis; photodynamic therapy; suction methods; scarring; and finally the recent and doubtlessly future introduction of home devices for use in a range of dermatologic applications (depilation, rejuvenation, treatment of acne, etc).

Pancreatic panniculitis: an atypical clinical presentation

Auteur(s) : Lorea Bagazgoitia1, Teresa Alonso1, Luis Rios-Buceta1, Angel Ruedas2, Rosario Carrillo3, Ernesto Munoz-Zato1 1Dermatology department 2Internal Medicine department 3Pathology department, Hospital Ramon y Cajal. Madrid. Universidad de Alcala de Henares. Carretera de Colmenar, km 9,1, 28034 Madrid, Spain Pancreatic panniculitis is a rare condition caused by inflammation of subcutaneous tissue, occurring in 2-3% of the patients with pancreatic disease [1]. It consists of brownish-red, tender, [...]

Subcutaneous emphysema caused by cryotherapy application over a corticosteroid-induced atrophic skin.

508

Multiple basal cell carcinomas arising on a thermal-burn scar. Successful treatment with photodynamic therapy

© 2008 The Authors JEADV 2009, 23, 441–496 Journal compilation