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Featured researches published by Lorena Bavia.


Acta Ophthalmologica | 2010

Association of complement factor H Y402H polymorphism and age-related macular degeneration in Brazilian patients

Anderson Teixeira; Aldacilene S. Silva; Fabio Lin; Roberta Velletri; Lorena Bavia; Rubens Belfort; Lourdes Isaac

Purpose:  The aim of this study was investigate the association between complement Factor H polymorphism (Y402H) and age‐related macular degeneration (AMD) in Brazilian patients.


Mediators of Inflammation | 2015

C57BL/6 and A/J Mice Have Different Inflammatory Response and Liver Lipid Profile in Experimental Alcoholic Liver Disease

Lorena Bavia; Íris Arantes de Castro; Lourdes Isaac

Alcoholic liver disease (ALD) is an important worldwide public health issue characterized by liver steatosis, inflammation, necrosis, and apoptosis of hepatocytes with eventual development of fibrosis and cirrhosis. Comparison of murine models with different inflammatory responses for ALD is important for an evaluation of the importance of genetic background in the interpretation of ethanol-induced phenotypes. Here, we investigated the role of inflammation and genetic background for the establishment of ALD using two different mouse strains: C57BL/6 (B6) and A/J. B6 and A/J mice were treated with a high fat diet containing ethanol (HFDE) and compared to the controls for 10 weeks. Hepatomegaly and steatohepatitis were similar in B6 and A/J mice, but only A/J mice were resistant to weight gain. On the other hand, HFDE-fed B6 accumulated more triglycerides (TG) and cholesterol and presented more intense cellular infiltrate in the liver when compared to HFDM-fed mice. Liver inflammatory environment was distinct in these two mouse strains. While HFDE-fed B6 produced more liver IL-12, A/J mice increased the TNF-α production. We concluded that mouse genetic background could dictate the intensity of the HFDE-induced liver injury.


Immunobiology | 2016

Complement C5 controls liver lipid profile, promotes liver homeostasis and inflammation in C57BL/6 genetic background

Lorena Bavia; Íris Arantes de Castro; Bruno Cogliati; Juliano Bertollo Dettoni; Venancio Avancini Ferreira Alves; Lourdes Isaac

Innate immunity contributes effectively to the development of alcoholic liver disease (ALD). In special, the activation of the complement system is involved in the pathogenesis of this disease. Here we investigated the contribution of complement C5 protein to the establishment and maintenance of ALD. Eight- to ten-week-old B6C5(+) and B6C5(-) male mice were fed with high fat diet (HFD) only or the same diet containing equicaloric supplements of ethanol (HFDE) or maltodextrin (HFDM) for 10 weeks. Serum parameters of liver function as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AP), albumin, glucose, triglycerides (TG) and cholesterol were evaluated. Liver tissue samples were collected for histopathological analysis, lipid extraction (TG and cholesterol), cytokines (TNF-α, IL-6, IL-1β, IL-10, IL-12, IL-17, IFN-γ, TGF-β) measurement and NO production. We observed that B6C5(-) mice HFDE-fed accumulated more liver cholesterol and TG, increased liver IL-17 and IL-10 levels and reduced liver TGF-β levels when compared to HFD-fed mice. We also observed that serum AST, AP and albumin were increased in B6C5(-) mice. Liver IL-1β, IL-6, IL-12 and IFN-γ were decreased in B6C5(-) mice independently of diet. We conclude that C5 acts in the control of serum TG and cholesterol, liver cholesterol deposition, liver homeostasis and C5 promotes a pro-inflammatory liver environment in our mouse model of ALD.


Immunology Letters | 2014

Basal physiological parameters of two congenic mice strains: C5 deficient C57BL/6 and C5 sufficient A/J

Lorena Bavia; Íris Arantes de Castro; Silvia Maria Gomes Massironi; Lourdes Isaac

To investigate the in vivo role of complement component C5 it is common to compare the inflammatory response between C5-normal and C5-deficient inbred mice strains. Nevertheless, it should be expected that differences in the genetic backgrounds between those strains may affect several physiological parameters, complicating the correct interpretation of results. The use of congenic mice, developed by backcrossing, is therefore preferred. Still, several physiological parameters may be distinctive in the normal and deficient strains and therefore require careful analysis before animals are selected for investigation. We generated two congenic mouse strains: C57BL/6 (B6) C5(-), derived from the parental B6 C5(+) strain and A/J C5(+) mice derived from the parental A/J C5(-) strain. After confirmation by nucleotide sequencing, immunodiffusion and hemolytic activity analysis, several basal physiological parameters were analyzed in the congenic and parental strains before antigen exposition. Serum levels of liver alanine aminotransferase, alkaline phosphatase, albumin, triglycerides, cholesterol and uric acid were found to be different in C5-sufficient and C5-deficient mice from one or both genetic backgrounds (B6 and/or A/J). On the other hand, serum levels of liver aspartate aminotransferase, glucose and urea were not affected by the presence of C5 in either strain. Furthermore, in some cases, C5-dependent variations in these parameters were more evident in mice of the same gender. We conclude here that C5-deficient mice strains may present distinct systemic behaviors which should be taken in consideration before differences in the immune responses are attributed solely to the lack of circulating C5.


Immunology Letters | 2016

The complement component C5 promotes liver steatosis and inflammation in murine non-alcoholic liver disease model.

Lorena Bavia; Bruno Cogliati; Juliano Bertollo Dettoni; Venancio Avancini Ferreira Alves; Lourdes Isaac

Non-Alcoholic Fatty Liver Disease (NALD) is considering a hepatic manifestation of metabolic syndrome. Although the pathogenesis of NALD is not completely understood, insulin resistance and inflammatory cytokines are implicated. Considering that component C5 is a central mediator of inflammation, we investigated the role of C5 in the establishment of NALD. Eight to ten-week old B6 C5(+) and A/J C5(-) male mice were fed a high fat diet containing glucose (HFDG) for 6 and 10 weeks. We observed that B6 C5(+) mice HFDG-fed for 10 weeks developed hepatomegaly, triglycerides (TG) accumulation, steatosis and enhanced liver TNF-α, IL-6, IL-12p70 and IL-17 levels when compared to A/J C5(-) mice. Next, B6 C5(+) mice were compared with congenic B6 C5(-) mice. Again, B6 C5(+) HFDG-fed mice developed more steatosis, liver centro-lobular inflammation and presented higher levels of liver IL-1β, IL-12p70, IL-17 and TFG-β than B6 C5(-) mice under the same conditions. B6 C5(+) mice HFDG-fed also presented lower concentrations of serum albumin, serum cholesterol, blood leukocytes and liver NO production when compared with B6 C5(-) mice. We concluded that murine C5 contributes effectively to liver steatosis and inflammation in NALD pathogenesis. In addition, C5 is also important to control serum cholesterol and albumin levels in the C57BL/6 genetic background.


Frontiers in Cellular and Infection Microbiology | 2018

Role of Murine Complement Component C5 in Acute in Vivo Infection by Pathogenic Leptospira interrogans

Íris Arantes de Castro; Lorena Bavia; Tatiana R. Fraga; Mariane T. Amano; Leandro Carvalho Dantas Breda; Adriana P. Granados-Martinez; Ana Maria Gonçalves da Silva; Silvio Arruda Vasconcellos; Lourdes Isaac

Leptospirosis is considered one of the most important zoonosis worldwide. The activation of the Complement System is important to control dissemination of several pathogens in the host. Only a few studies have employed murine models to investigate leptospiral infection and our aim in this work was to investigate the role of murine C5 during in vivo infection, comparing wild type C57BL/6 (B6 C5+/+) and congenic C57BL/6 (B6 C5−/−, C5 deficient) mice during the first days of infection. All animals from both groups survived for at least 8 days post-infection with pathogenic Leptospira interrogans serovar Kennewicki strain Fromm (LPF). At the third day of infection, we observed greater numbers of LPF in the liver of B6 C5−/− mice when compared to B6 C5+/+ mice. Later, on the sixth day of infection, the LPF population fell to undetectable levels in the livers of both groups of mice. On the third day, the inflammatory score was higher in the liver of B6 C5+/+ mice than in B6 C5−/− mice, and returned to normal on the sixth day of infection in both groups. No significant histopathological differences were observed in the lung, kidney and spleen from both infected B6 C5+/+ than B6 C5−/− mice. Likewise, the total number of circulating leukocytes was not affected by the absence of C5. The liver levels of IL-10 on the sixth day of infection was lower in the absence of C5 when compared to wild type mice. No significant differences were observed in the levels of several inflammatory cytokines when B6 C5+/+ and B6 C5−/− were compared. In conclusion, C5 may contribute to the direct killing of LPF in the first days of infection in C57BL/6 mice. On the other hand, other effector immune mechanisms probably compensate Complement impairment since the mice survival was not affected by the absence of C5 and its activated fragments, at least in the early stage of this infection.


Molecular Vision | 2012

Plasma levels of complement proteins from the alternative pathway in patients with age-related macular degeneration are independent of Complement Factor H Tyr402His polymorphism

Aldacilene S. Silva; Anderson Teixeira; Lorena Bavia; Fabio Lin; Roberta Velletri; Rubens Belfort; Lourdes Isaac


Revista da Sociedade Brasileira de Ciência em Animais de Laboratório | 2013

A influência do fundo genético de camundongos C57Bl/6 e A/J na resposta inflamatória aguda induzida pelo etanol

Lorena Bavia; Paulo César Maiorka; Lourdes Isaac


Archive | 2013

ThE infLUEnCE Of gEnETiC BACkgROUnd Of C57BL/6 And A/J M i CE O n T h E d E v ELOPME n T O f ACUTE inf LAMMATOR y RESPO n SE ind UCE d B y ALCO h OL

Lourdes Isaac; Lorena Bavia; Paulo César Maiorka


Immunobiology | 2012

The interference of genetic background of C57Bl/6 and A/J mice on the development of acute and chronic inflammatory response induced by ethanol: Possible contribution of C5

Lorena Bavia; Lourdes Isaac

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Lourdes Isaac

University of São Paulo

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Anderson Teixeira

Federal University of São Paulo

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Bruno Cogliati

University of São Paulo

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Fabio Lin

University of São Paulo

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Roberta Velletri

Federal University of São Paulo

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Rubens Belfort

Federal University of São Paulo

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