Lorena Tannús Menezes

Ontogeny of the Appendicular Skeleton in Melanosuchus niger (Crocodylia: Alligatoridae)

The objective of the present study was to analyze chondrogenesis and the ossification pattern of the limbs of Melanosuchus niger in order to contribute with possible discussions on homology and the fusion pattern of autopodial elements and phylogeny. In the Reserva Extrativista do Lago Cuniã, Rondônia, Brazil, six nests were marked and two eggs removed from each nest at 24-hour intervals until hatching. Embryos were cleared using KOH; bone tissue was stained with alizarin red S and cartilage with Alcian blue. Routine staining with HE was also performed. In the pectoral girdle, the scapula showed ossification centers before the coracoid process. In the pelvic girdle, the ilium and the ischium were condensed as a single cartilage, although ossification took place through two separate centers, forming distinct elements in the adult. The pubis developed from an independent cartilaginous center with free end, which reflects its function in breathing. In the initial stages, the stylopodium and the zeugopodium developed from the condensation of a Y-shaped cartilage in the limbs, and differentiation of the primary axis and digital arch were observed. The greatest changes were observed in the mesopodia. In their evolution, Crocodylia underwent a vast reduction in the number of autopodial elements as a consequence of fusions and ossification of some elements. This study shows that the chondrogenesis and ossification sequences are dissociated. Moreover, the differences between M. niger and other species show clear variation in the patterns for these events in Alligatoridae.

Ontogeny of the Postcranial Axial Skeleton of Melanosuchus niger (Crocodylia, Alligatoridae)

This study proposes the description of the development of the postcranial axial skeleton, including vertebrae, gastralium, ribs, sternum, and interclavicle, in Melanosuchus niger. Six nests were marked and two eggs removed from each nest at 24‐hr intervals until hatching. For posthatching evaluation, 30 hatchlings were kept in captivity and one exemplar was euthanized at three‐day intervals. Samples were diaphanized using potassium hydroxide (KOH), alizarin red S, and Alcian blue. A routinely generally used method was applied for histological evaluation. It was difficult to define in which vertebrae the development of cartilaginous centers began, but it was possible to observe that this condensation advanced in the craniocaudal direction. The condensation started in the vertebral arches and was visibly stronger in the cervical and dorsal regions, advancing to the lumbar, sacral and, last, to the caudal region. The atlas showed a highly different morphology compared with the other cervical vertebrae, with a short intercenter, two neural arches, and a proatlas. The ossification process began in the body of cervical vertebrae III to VIII and alizarin retention decreased in the last vertebrae, indicating a craniocaudal direction in bone development, similar to cartilage formation. In the histological sections of gastralium and interclavicles of M. niger at several development stages, it was possible to observe that these elements showed intramembranous development. Anat Rec, 301:607–623, 2018.

Topografia do cone medular de Ouriço-cacheiro (Coendou prehensilis, Linnaeus, 1758) (Rodentia)

The first proposed of caudal anesthesia was in 1926 and many techniques were developed to anesthetize the lumbar and sacral spinal nerves. This study determined the medullary cone topography of the Coendou prehensilis, using six adults from the Sabiá Park Zoo, Uberlândia-MG. The material was fixed in formaldehyde solution 10% in the LAPAS. The skin incision was done, then the spine muscles were removed and the vertebral arches were cut to visualize the spinal cord and the surrounding structures. The medullary cone in all the specimens began in the fourth lumbar vertebra (L4) and finished in the second sacral vertebra (S2), and the length changed to 5.9cm to 4.7cm. This study concluded that the best place for epidural anesthesia of the pelvic limb in the Coendou prehensilis is between the L4 and S2 vertebrae.