Lothar Beck

kette and blown fuse interact genetically during the second fusion step of myogenesis in Drosophila.

Drosophila myoblast fusion proceeds in two steps. The first one gives rise to small syncytia, the muscle precursor cells, which then recruit further fusion competent myoblasts to reach the final muscle size. We have identified Kette as an essential component for myoblast fusion. In kette mutants, founder cells and fusion-competent myoblasts are determined correctly and overcome the very first fusion. But then, at the precursor cell stage, fusion is interrupted. At the ultrastructural level, fusion is characterised by cell-cell recognition, alignment, formation of prefusion complexes, electron dense plaques and membrane breakdown. In kette mutants, electron dense plaques of aberrant length accumulate and fusion is interrupted owing to a complete failure of membrane breakdown. Furthermore, we show that kette interacts genetically with blown fuse (blow) which is known to be required to proceed from prefusion complexes to the formation of the electron dense plaques. Interestingly, a surplus of Kette can replace Blow function during myogenesis. We propose a model in which Dumbfounded/Sticks and stones-dependent cell adhesion is mediated over Rolling Pebbles, Myoblast city, Crk, Blown fuse and Kette, and thus induces membrane fusion.

WASP and SCAR have distinct roles in activating the Arp2/3 complex during myoblast fusion

Myoblast fusion takes place in two steps in mammals and in Drosophila. First, founder cells (FCs) and fusion-competent myoblasts (FCMs) fuse to form a trinucleated precursor, which then recruits further FCMs. This process depends on the formation of the fusion-restricted myogenic-adhesive structure (FuRMAS), which contains filamentous actin (F-actin) plugs at the sites of cell contact. Fusion relies on the HEM2 (NAP1) homolog Kette, as well as Blow and WASP, a member of the Wiskott-Aldrich-syndrome protein family. Here, we show the identification and characterization of schwächling – a new Arp3-null allele. Ultrastructural analyses demonstrate that Arp3schwächling mutants can form a fusion pore, but fail to integrate the fusing FCM. Double-mutant experiments revealed that fusion is blocked completely in Arp3 and wasp double mutants, suggesting the involvement of a further F-actin regulator. Indeed, double-mutant analyses with scar/WAVE and with the WASP-interacting partner vrp1 (sltr, wip)/WIP show that the F-actin regulator scar also controls F-actin formation during myoblast fusion. Furthermore, the synergistic phenotype observed in Arp3 wasp and in scar vrp1 double mutants suggests that WASP and SCAR have distinct roles in controlling F-actin formation. From these findings we derived a new model for actin regulation during myoblast fusion.

The smell of success: choice of larval rearing sites by means of chemical cues in a Peruvian poison frog

Parental care is a common strategy among vertebrates to ensure successful reproduction. Anuran amphibians have evolved a remarkable diversity of reproductive methods including advanced levels of parental care. Among the most derived strategies are those of the Neotropical poison frogs (Dendrobatidae). These amphibians exhibit a wide array of behavioural traits such as egg guarding, larval transport by parental frogs and larval feeding with trophic (unfertilized) eggs. Ranitomeya variabilis from the upper Amazon basin in Peru deposits both eggs and tadpoles in phytotelmata. The exploitation of these small pools is advantageous as it lowers the risk of predation, but it is more costly because of limited resource availability. Additionally, poison frog larvae are often cannibalistic, so the identification and avoidance of conspecifics represents an adaptive behaviour for these amphibians. While studies have shown that poison frogs actively avoid depositing with conspecifics, the mechanism for assessing pool quality remains unknown. In field experiments, we found that parental R. variabilis frogs used chemical cues to recognize the presence of tadpoles in phytotelmata. Furthermore, they distinguished between cannibalistic and noncannibalistic tadpoles, a behaviour that supports the survival of their own offspring. 2011 The Association for the Study of Animal Behaviour. Published by Elsevier Ltd. All rights reserved.

The Arf-GEF Schizo/Loner regulates N-cadherin to induce fusion competence of Drosophila myoblasts.

Myoblast fusion is a key process in multinucleated muscle formation. Prior to fusion, myoblasts recognize and adhere to each other with the aid of cell-adhesion proteins integrated into the membrane. Their intracellular domains participate in signal transduction by binding to cytoplasmic proteins. Here we identified the calcium-dependent cell-adhesion protein N-cadherin as the binding partner of the guanine-nucleotide exchange factor Schizo/Loner in Drosophila melanogaster. N-cadherin was expressed in founder cells and fusion-competent myoblasts of Drosophila during the first fusion phase. Our genetic analyses demonstrated that the myoblast fusion defect of schizo/loner mutants is rescued in part by the loss-of-function mutation of N-cadherin, which suggests that Schizo/Loner is a negative regulator of N-cadherin. Based on our findings, we propose a model where N-cadherin must be removed from the myoblast membrane to induce a protein-free zone at the cell-cell contact point to permit fusion.

Drosophila Kette coordinates myoblast junction dissolution and the ratio of Scar-to-WASp during myoblast fusion

ABSTRACT The fusion of founder cells and fusion-competent myoblasts (FCMs) is crucial for muscle formation in Drosophila. Characteristic events of myoblast fusion include the recognition and adhesion of myoblasts, and the formation of branched F-actin by the Arp2/3 complex at the site of cell–cell contact. At the ultrastructural level, these events are reflected by the appearance of finger-like protrusions and electron-dense plaques that appear prior to fusion. Severe defects in myoblast fusion are caused by the loss of Kette (a homolog of Nap1 and Hem-2, also known as NCKAP1 and NCKAP1L, respectively), a member of the regulatory complex formed by Scar or WAVE proteins (represented by the single protein, Scar, in flies). kette mutants form finger-like protrusions, but the electron-dense plaques are extended. Here, we show that the electron-dense plaques in wild-type and kette mutant myoblasts resemble other electron-dense structures that are known to function as cellular junctions. Furthermore, analysis of double mutants and attempts to rescue the kette mutant phenotype with N-cadherin, wasp and genes of members of the regulatory Scar complex revealed that Kette has two functions during myoblast fusion. First, Kette controls the dissolution of electron-dense plaques. Second, Kette controls the ratio of the Arp2/3 activators Scar and WASp in FCMs. Summary: The Drosophila protein Kette is essential for myoblast fusion. It controls the dissolution of electron-dense plaques and the ratio of Scar and WASp proteins in fusion-competent myoblasts during fusion pore formation.

MARBURG: Zoological Collection of the Philipps University of Marburg

The Zoological Collection of the Philipps University of Marburg was founded in 1817–1819 and has existed continuously to this day, surviving both world wars mostly undamaged. It is subdivided into a reference collection, a teaching collection and a public collection. The entire collection consists of approximately 40,000 specimens with over 20,000 insects and other arthropods, 3500 further invertebrates (marine species, terrestrial molluscs, parasites), over 650 birds and mammals, about 300 reptiles and amphibians and more than 630 skeletons, animal and human skulls. Furthermore, it owns 5800 microscope slides dating back to the nineteenth century as well as about 600 didactic wall maps. Among its special features are the complete mounted skeleton of an Asian elephant, several mummy skulls, many special molluscs and the coleopteran collection of C. F. Riehl. Today, the Zoological Collection is still used for teaching purposes and serves as a reference collection or resource for exhibitions. In addition, ongoing research is conducted within the collection, in connection with student theses or focusing on the history of the collection or on its specimens. In this context, for example, old department files are being processed and digitalised. At times, certain specimens of the collection participate in both regional and national exhibitions. Guided tours of the collection are available on request.