Louis B. Hoff
Applied Biosystems
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Publication
Featured researches published by Louis B. Hoff.
Genomics | 1992
Janet S. Ziegle; Ying Su; Kevin P. Corcoran; Li Nie; P. Eric Mayrand; Louis B. Hoff; Lincoln Mcbride; Mel N. Kronick; Scott R. Diehl
Highly polymorphic microsatellite loci offer great promise for gene mapping studies, but fulfillment of this potential will require substantial improvements in methods for accurate and efficient genotyping. Here, we report a genotyping method based on fluorescently labeled PCR primers and size characterization of PCR products using an automated DNA fragment analyzer. We capitalize on the availability of three distinct fluorescent dyes to label uniquely loci that overlap in size, and this innovation increases by threefold the number of loci that can be analyzed simultaneously. We label size standards with a fourth dye and combine these with the microsatellite PCR products in each gel lane. Computer programs provide very rapid and accurate sizing of microsatellite alleles and efficient data management. In addition, fluorescence signals are linear over a much greater range of intensity than conventional autoradiography. This facilitates multiplexing of loci (since signal intensities often vary greatly) and helps distinguish major peaks from artifacts, thereby improving genotyping accuracy.
Analytical Biochemistry | 1987
J.Scott Eadie; Lincoln Mcbride; J William Efcavitch; Louis B. Hoff; Richard Cathcart
A significantly improved method for base composition analysis of synthetic oligodeoxyribonucleotides is presented. This highly accurate and sensitive method used enzymatic digestion followed by high-resolution HPLC of the nucleosides to determine the empirical base composition of the parent compound. The enzymatic digestion reaction is quantitative and is not blocked by modified bases, thus allowing the degree of base deprotection and chemical modification to be assessed. Digestion data are presented for oligodeoxyribonucleotides which range from 18 to 150 bases in length with excellent agreement of experimental and theoretical composition. The method is also applicable to high-molecular-weight genomic DNA.
Archive | 1992
G. Richard Cathcart; Thomas Brennan-Marquez; John Bridgham; George Golda; Harry Guiremand; Marianne Hane; Louis B. Hoff; Eric Lachenmeier; Melvyn N. Kronick; Douglas H. Keith; Paul Mayrand; Michael Metzker; William Mordan; Lincoln Mcbride; John Shigeura; Chen Hanson Ting; Norman M. Whiteley
Archive | 2004
Louis B. Hoff; Mark F. Oldham
Archive | 1995
Louis B. Hoff; Eric Lachenmeier; Yefim Raysberg; Eric S. Nordman
Archive | 1992
John Shigeura; John Bridgham; Louis B. Hoff; P. Eric Mayrand
Archive | 1995
Paul A Hing; Louis B. Hoff; Eric Lachenmeier; Dennis E Mead; Barry A Simkins; J William Efcavitch
Archive | 1991
Guy Richard Cathcart; Thomas Brennan-Marquez; John Bridgham; George Golda; Harry Guiremand; Marianne Hane; Louis B. Hoff; Eric Lachenmeier; Melvyn N Kronick; Douglas H. Keith; Paul Mayrand; Michael Metzker; William Mordan; Lincoln Mcbride; John Shigeura; Chen Ting; Norman M. Whiteley
Archive | 2002
Louis B. Hoff; Mark F. Oldham
Archive | 2002
Louis B. Hoff; Mark F. Oldham