Louise Kuchel

Routine and Active Metabolic Rates of Migrating Adult Wild Sockeye Salmon (Oncorhynchus nerka Walbaum) in Seawater and Freshwater

We present the first data on the differences in routine and active metabolic rates for sexually maturing migratory adult sockeye salmon (Oncorhynchus nerka) that were intercepted in the ocean and then held in either seawater or freshwater. Routine and active oxygen uptake rates (Mo2) were significantly higher (27%–72%) in seawater than in freshwater at all swimming speeds except those approaching critical swimming speed. During a 45‐min recovery period, the declining postexercise oxygen uptake remained 58%–73% higher in seawater than in freshwater. When fish performed a second swim test, active metabolic rates again remained 28%–81% higher for fish in seawater except at the critical swimming speed. Despite their differences in metabolic rates, fish in both seawater and freshwater could repeat the swim test and reach a similar maximum oxygen uptake and critical swimming speed as in the first swim test, even without restoring routine metabolic rate between swim tests. Thus, elevated Mo2 related to either being in seawater as opposed to freshwater or not being fully recovered from previous exhaustive exercise did not present itself as a metabolic loading that limited either critical swimming performance or maximum Mo2. The basis for the difference in metabolic rates of migratory sockeye salmon held in seawater and freshwater is uncertain, but it could include differences in states of nutrition, reproduction, and restlessness, as well as ionic differences. Regardless, this study elucidates some of the metabolic costs involved during the migration of adult salmon from seawater to freshwater, which may have applications for fisheries conservation and management models of energy use.

Turning up the heat on subzero fish: thermal dependence of sustained swimming in an Antarctic notothenioid

We determined the maximum sustained swimming speed (U-crit), and resting and maximum ventilation rates of the Antarctic fish Pagothenia borchgrevinki at five temperatures between -1degreesC and 8degreesC. We also determined resting metabolic rate (VO2) at -1degreesC, 2degreesC, and 4degreesC. U-crit of P. borchgrevinki was highest at -1degreesC (2.7+/-0.1 BL s(-1)) and rapidly decreased with temperature, representing a thermal performance breadth of only 5degreesC. This narrow thermal performance supports our prediction that specialisation to the subzero Antarctic marine environment is associated with a physiological trade-off in performance at high temperatures. Resting oxygen consumption and ventilation rate increased by more than 200% across the temperature range, which most likely contribute to the decrease in aerobic swimming capabilities at higher temperatures

Intrinsic mechanical properties of the perfused armoured catfish heart with special reference to the effects of hypercapnic acidosis on maximum cardiac performance

SUMMARY The armoured catfish, Pterygoplichthys pardalis, is known to be extremely tolerant of environmental hypercarbia (elevated water CO2 tensions), which occurs in their natural environment. In addition, previous studies have demonstrated that during exposure to hypercarbia, P. pardalis does not exhibit extracellular pH compensation and thus the heart and other organs must continue to function despite a severe extracellular acidosis. We used an in situ perfused heart preparation to determine the effects of an extracellular hypercapnic (elevated CO2 in the animal) acidosis (1–7.5% CO2) on heart function, specifically cardiac output, power output, heart rate and stroke volume. The present study is the first to comprehensively examine cardiac function in an acidosis-tolerant teleost. When compared with control conditions, maximum cardiac performance was unaffected at levels of CO2 as high as 5%, far exceeding the hypercapnic tolerance of other teleosts. Moreover, P. pardalis exhibited only a moderate decrease (∼35%) in cardiac performance when exposed to 7.5% CO2, and full cardiac performance was restored in six out of seven hearts upon return to control conditions. Myocardial intracellular pH (pHi) was protected in situ, as has been found in vivo, and this protection extended to the highest level of CO2 (7.5%) investigated. Thus, maintained heart function during a hypercapnic acidosis in P. pardalis is probably associated with preferential pHi regulation of the heart, but ultimately is not sufficient to prevent loss of cardiac function. Our findings suggest the need for further study to elucidate the mechanisms behind this remarkable cardiac hypercapnic tolerance.

Seasonal Metabolic Acclimatization in a Northern Population of Free-Ranging Snowshoe Hares, Lepus americanus

Abstract Seasonal acclimatization in high-latitude endotherms may involve increases or reductions in body size and metabolic rate to, respectively, augment thermoregulatory capacity or reduce energy requirements. We investigated seasonal acclimatization in a northern population of wild snowshoe hares (Lepus americanus) that is exposed to low food availability and extremely cold temperatures in winter. Snowshoe hares were livetrapped and transported to a nearby mobile laboratory. Hares were placed in a metabolic chamber and oxygen consumption was measured for 55 min at each of the following temperatures: 10°C, 0°C, −10°C, −15°C, and −20°C. Hair length and density were measured on a sample of collected hares. Snowshoe hares maintained similar body mass and body temperature between the seasons, but average resting metabolic rate and thermal conductance were, respectively, 20% and 32% lower in winter than in autumn. The lower critical temperature was −10°C to −15°C in winter and 0°C to −10°C in autumn. Guard hairs were 36% longer and 148% denser in winter than autumn, whereas downy hairs were the same length but 128% denser in winter than autumn. Collectively, these results suggest that resource constraints associated with a herbivorous diet in regions and seasons of poor forage quality favors an energetically conservative approach to winter acclimatization.

Morphology of the cloaca in the estuarine crocodile, Crocodylus porosus, and its plastic response to salinity.

The cloacal complex of Crocodylus porosus is composed of three chambers (proctodaeum, urodaeum, and coprodaeum) separated by tight, muscular sphincters. The proctodaeum is proximal to the cloacal vent and houses the genitalia. The urodaeum is the largest chamber, is capable of storing large quantities of urine, and is lined with an epithelium with the capacity for transepithelial water and ion exchange. The coprodaeum, the most orad cloacal chamber, is a small, only marginally expandable chamber that has an epithelium composed almost entirely of mucus‐secreting cells. The coprodaeum and lower intestine are reported to be the site(s) for urine modification in birds and bladderless lizards. A radiographic trace of urine storage in C. porosus kept for 2 months under hyperosmotic conditions showed no signs of retrograde movement of urine into the coprodaeum or rectum. Instead, urine was stored in the urodaeum of C. porosus. Examination of the mucosal surface of the urodaeum by SEM showed a plastic response to environmental salinity, with a possible increase in surface area in animals kept in hyperosmotic water compared with animals from fresh water. We propose the urodaeum as the primary site for postrenal modification of urine in C. porosus. J. Morphol. 245:168–176, 2000

The cold shoulder: free-ranging snowshoe hares maintain a low cost of living in cold climates

The hypothesis that cold air temperatures (Ta) constrain the metabolic diversity of high-latitude endotherms is based on the observation among birds and mammals that mean field metabolic rate (FMR) increases, whereas the variability of FMR decreases, from the warm tropics to the cold poles. However, there is a paucity of FMR measurements from above 60° latitude and below 0 °C. We measured the daily energy expenditure of a high-latitude population of free-ranging snowshoe hares (Lepus americanus Erxleben, 1777) in Yukon, Canada, in winter (Ta-mean = –16.4 °C) and in autumn (Ta-mean = 0.5 °C). Doubly labelled water measures of FMR were approximately 20% lower in winter than in autumn, and were a similar, low multiple of resting metabolic rate in both seasons (2.04 and 1.94, respectively). The mass-corrected FMR of snowshoe hares in winter was only half the value predicted by extrapolating the relationship between FMR and Ta > 0 to –16.4 °C. These results contribute to an emerging pattern of a reversal in the relationship between FMR and Ta in free-ranging mammals from negative above 0 °C to positive below 0 °C. We refer to the positive, low Ta portion of this relationship as the cold shoulder, and suggest that it may reflect the general necessity for free-ranging mammals to use behavioural and (or) physiological means to conserve energy during long winters when cold conditions coincide with resource scarcity.

Teaching Scientists to Communicate: Evidence-based assessment for undergraduate science education

Communication skills are one of five nationally recognised learning outcomes for an Australian Bachelor of Science (BSc) degree. Previous evidence indicates that communication skills taught in Australian undergraduate science degrees are not developed sufficiently to meet the requirements of the modern-day workplace—a problem faced in the UK and USA also. Curriculum development in this area, however, hinges on first evaluating how communication skills are taught currently as a base from which to make effective changes. This study aimed to quantify the current standard of communication education within BSc degrees at Australian research-intensive universities. A detailed evidential baseline for not only what but also how communication skills are being taught was established. We quantified which communication skills were taught and assessed explicitly, implicitly, or were absent in a range of undergraduate science assessment tasks (n = 35) from four research-intensive Australian universities. Results indicate that 10 of the 12 core science communication skills used for evaluation were absent from more than 50% of assessment tasks and 77.14% of all assessment tasks taught less than 5 core communication skills explicitly. The design of assessment tasks significantly affected whether communication skills were taught explicitly. Prominent trends were that communication skills in tasks aimed at non-scientific audiences were taught more explicitly than in tasks aimed at scientific audiences, and the majority of group and multimedia tasks taught communication elements more explicitly than individual, or written and oral tasks. Implications for science communication in the BSc and further research are discussed.

Kidney and Cloaca Function in the Estuarine Crocodile (Crocodylus porosus) at Different Salinities: Evidence for Solute-linked Water Uptake

Kidney function and the role of the cloacal complex in osmoregulation was investigated in estuarine crocodile (Crocodylus porosus) exposed to three environmental salinities: hypo-, iso- and hyperosmotic to the plasma. Plasma homeostasis was maintained over the range of salinities. Antidiuresis occurred with increased salinity. Although urine from the kidneys retained an osmotic pressure between 77% and 82% of the plasma, over 93% and 98% of plasma chloride filtered at the glomeruli was reabsorbed during passage through the kidneys under hypo and hyperosmotic conditions, respectively, and only 64% in iso-osmotic water. The kidneys were the primary site of sodium reabsorption under hypo-and hyperosmotic conditions. Secondary processing of urine during storage in the cloaca varied with salinity. During post renal storage of urine, the difference in urine osmotic pressure increased from -26.1 +/- 15.5 to 35.66 +/- 9.29 mOsM with increased salinity, and potassium concentration of urine increased over 3-fold in C. porosus from freshwater. The almost complete reabsorption of both sodium and chloride under hyperosmotic conditions indicates the necessity for secretory activity by the lingual salt glands. The osmoregulatory response of the kidneys and cloacal complex to environmental salinity is both plastic and complementary

Framing renewable energy: a comparative study of newspapers in Australia and Sweden

Australia and Sweden display very different institutional settings and contexts for the production of environmental journalism. This empirical study examined how two major quality newspapers in Sweden and Australia have framed renewable energy as an environmental, political, scientific, economic and civil society issue. A deductive, quantitative methodological approach was used to identify dominant frames and actors in articles in The Australian (Australia) and Dagens Nyheter (Sweden) between October 2010 and June 2011 2010/2011. The findings suggest that the attention given to different types of renewable energy in the two newspapers and how issues were framed was contingent on the domestication of the discussion of renewable energy in the two countries. Reporting on renewable energy in both newspapers was characterized by a focus on “elite” actors and economic frames, the absence of civil society frames and negative (The Australian) or ambiguous (Dagens Nyheter) environmental frames. The study extends our understanding of the contextual conditions that enable and limit journalists when reporting environmental issues.

Metabolic responses of the South American ornate horned frog (Ceratophrys ornata) to estivation

We examined the metabolic responses of the South American frog, Ceratophrys ornata, to laboratory-induced estivation. Whole-animal and mass-specific oxygen consumption rates (VO(2)) did not change during fasting or 56days of estivation, despite observing significant decreases in body mass. The maintenance of mass-specific metabolic rate at routine levels during estivation suggests that metabolic rate suppression is not a major response to estivation in this species. There was a significant decline in liver glycogen and a loss of adipose tissue mass during estivation, suggesting that both carbohydrate and lipid pathways are used to fuel metabolism during estivation. The activity of pyruvate dehydrogenase, an important regulator of carbohydrate oxidation, and carnitine palmitoyltransferase and 3-hydroxyacyl-CoA dehydrogenase, regulators of lipid oxidation, showed no significant change in activity in liver, heart, and muscle between estivating and active frogs. There was an increase in plasma osmolality, which is characteristic of estivating animals. Overall, our metabolic analysis of estivation in C. ornata indicates that this species does not employ a dramatic suppression metabolic rate to survive dehydration stress and that both endogenous carbohydrates and lipids are used as metabolic fuels.