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Featured researches published by Louise O'Connor.


Expert Review of Medical Devices | 2010

Recent advances in the development of nucleic acid diagnostics

Louise O'Connor; Barry Glynn

Since the early 1970s, the use of nucleic acid sequences for specific diagnostic applications has followed a somewhat linear pattern of development. Early methods for restriction enzyme digestion, as well as reverse transcription, were followed in the late 1970s by Southern, northern and dot blotting, as well as DNA sequencing. In 1985, the description of PCR and the routine laboratory manipulation of sufficient quantities of DNA for diagnostics, resulted in the exponential growth of molecular biology. Subsequently, alternative DNA and RNA amplification protocols followed. The last 10 years have seen the second explosion in molecular biology with the development of real-time quantitative PCR and oligonucleotide microarrays. This advancement continues with the development of methods for ‘direct’ nucleic acid target detection from samples without in vitro amplification, and enhanced transduction elements for improved sensitivity of nucleic acid detection. In this article, we will describe the current state of the art in nucleic acid diagnostics, the use of nucleic acid-based diagnostics in clinical practice and the emerging technologies in the field. Finally, we will describe future trends and expected advances in the field.


Journal of Food Protection | 2000

Rapid Polymerase Chain Reaction/DNA Probe Membrane-Based Assay for the Detection of Listeria and Listeria monocytogenes in Food

Louise O'Connor; Joy J; Marian Kane; Terry J. Smith; M. Maher

We describe the development of polymerase chain reaction (PCR)/DNA probe membrane-based colorimetric assays for the detection and identification of Listeria and L. monocytogenes. PCR primers designed from the 16S to 23S rRNA intergenic spacer region amplified products that were reverse hybridized to membrane-bound oligonucleotide probes specific for Listeria and L. monocytogenes with a detection limit of 1 to 10 CFU/25 ml in inoculated raw and pasteurized milk samples. These qualitative assays have the potential to be integrated into testing laboratories for monitoring the microbiological quality of foods.


Journal of Clinical Pathology | 2017

A novel molecular assay using hybridisation probes and melt curve analysis for CALR exon 9 mutation detection in myeloproliferative neoplasms

Thomas Keaney; Louise O'Connor; Janusz Krawczyk; Moutaz A Abdelrahman; Amjad Hayat; Margaret Murray; Michael O'Dwyer; Melanie J. Percy; Stehpen Langabeer; Karl Haslam; Barry Glynn; Ciara Mullen; Evelyn Keady; Sinead Lahiff; Terry J. Smith

Aims Somatic insertions/deletions in exon 9 of the calreticulin gene have been identified in patients with essential thrombocythemia and primary myelofibrosis. Over 55 mutations have been discovered, 80% of which consist of either type 1 52-bp deletion or type 2 5-bp insertion. Other mutations (types 3–5) in conjunction with types 1 and 2 account for >87% of identified mutations. The aim of this study was development of a rapid PCR-based assay using LightCycler Hybridisation Probes for the detection of type 1–5 CALR mutations. Method A real-time PCR assay using a novel HybProbe set was developed for use on the LightCycler 480 Instrument II. The acceptor probe was labelled with LC640 and Faststart DNA Master HybProbe kit was used for PCR reactions. Results Assay limit of detection was determined to be seven target copies with a probability of 95%. The specificity of the assay was determined by using synthetic constructs of CALR wild-type and CALR mutation types 1–5 with no non-specific detection observed. Samples from 21 patients with essential thrombocythemia (ET) and 12 patients with primary myelofibrosis (PMF), together with 29 control samples from patients diagnosed with various conditions, were screened using the assay. Of these, 24 were found to have mutations in CALR exon 9, with the assay detecting 8 type 1 mutations, 12 type 2 mutations, 2 type 24 mutations, 1 type 20 mutation and 1 31-bp deletion. Conclusions The novel assay described has potential for application as a rapid, sensitive, high-throughput screening method in the clinical diagnostics setting.


Molecular and Cellular Probes | 2005

Quantification of ALS1 gene expression in Candida albicans biofilms by RT-PCR using hybridisation probes on the LightCycler

Louise O'Connor; Sinead Lahiff; Fiona Casey; M. Glennon; Martin Cormican; Majella Maher


Irish Journal of Agricultural and Food Research | 2000

Molecular diagnostics in food safety: rapid detection of food-borne pathogens.

Terry J. Smith; Louise O'Connor; M. Glennon; M. Maher


Archive | 2009

SWI5 GENE AS A DIAGNOSTIC TARGET FOR THE IDENTIFICATION OF FUNGAL AND YEAST SPECIES

Thomas Barry; Majella Maher; Terry J. Smith; Marcin Jankiewicz; Louise O'Connor; Nina Tuite; Sinead Lahiff


Archive | 2006

Method for detecting c. albicans

Louise O'Connor; Majella Maher


Encyclopedia of Food Microbiology | 1999

MOLECULAR BIOLOGY IN MICROBIOLOGICAL ANALYSIS – DNA-BASED METHODS FOR THE DETECTION OF FOOD-BORNE PATHOGENS

Louise O'Connor; Majella Maher


Archive | 2017

A multiplex assay for the sensitive and specific detection and differentiation of Clostridium difficile

Terrence James Smith; Thomas Barry; Louise O'Connor


Archive | 2009

Ace2 as a target gene for the molecular identification of yeast and fungal species

Thomas Barry; Terry J. Smith; Majella Maher; Marcin Jankiewicz; Louise O'Connor; Nina Tuite; Sinead Lahiff

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Majella Maher

National University of Ireland

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Terry J. Smith

National University of Ireland

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Sinead Lahiff

National University of Ireland

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Thomas Barry

National University of Ireland

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Marcin Jankiewicz

National University of Ireland

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Nina Tuite

National University of Ireland

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Barry Glynn

National University of Ireland

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M. Glennon

National University of Ireland

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M. Maher

National University of Ireland

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Marian Kane

National University of Ireland

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