Love Kumar Soni

Anti-microbial benzimidazole derivatives: synthesis and in vitro biological evaluation

In this study, we have synthesized 5-(nitro/chloro)-2-styryl-benzimidazoles and evaluated for in vitro anti-microbial activity with different strain like anti-bacterial activity against Staphylococcus aureus, Escherichia coli, and anti-fungal activity against Candida albicans were carried out. Compounds 2, 4, 7, 8 showed higher activity against S. aureus, compounds 2, 8, 9, 10 showed higher activity against E. coli and Compounds 2, 7, 9, 8 showed higher activity against C. albicans.

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF METFORMIN HYDROCHLORIDE AND REPAGLINIDE IN TABLET DOSAGE FORM

A simple, rapid, and specific reversed-phase high performance liquid chromatographic method for simultaneous estimation of Metformin hydrochloride (MET) and Repaglinide (RPG) in a tablet dosage form has been developed and validated. HPLC analysis was performed on a C 18 column with 90:10 (v/v) acetonitrile-water as mobile phase at a flow rate of 1.0 mL min−1. UV detection was performed at 223 nm. Total run time was 10 min; Metformin hydrochloride and Repaglinide were eluted with retention times of 2.72 min and 6.13 min, respectively. The method was validated for accuracy, precision, linearity, specificity, and sensitivity in accordance with ICH guidelines. Validation revealed that the method is specific, rapid, accurate, precise, reliable, and reproducible. The high recovery and low coefficients of variation confirmed the suitability of the method for simultaneous analysis of the two drugs in tablet dosage form.

Synthesis and Antibacterial Screening of Some 1-Aroyl-3-aryl Thiourea Derivatives

A series of 1-aroyl-3-aryl thioureas derivatives were synthesized and evaluated for antibacterial activity. The results indicated that the compounds possessed higher activity against gram-negative bacteria than gram-positive bacteria. Amongst all these compounds, C18 (89.4%) exhibited the greatest antibacterial activity against gram-negative bacteria while C5 (85.6%) displayed maximum antibacterial activity against gram-positive bacteria. Preliminary study of the structure-activity relationship revealed that an electronic factor on aryl rings had a great effect on the antibacterial activity of these compounds.