Lu Zuhong

Polymorphisms in XRCC1 and XPG and response to platinum-based chemotherapy in advanced non-small cell lung cancer patients

Platinum-based chemotherapeutics is the most common regimens for advanced NSCLC patients. However, it is difficult to identify platinum resistance in clinical treatment. Genetic factors are thought to represent important determinants of drug efficacy. In this study, we investigated whether single nucleotide polymorphisms (SNPs) in Xeroderma pigmentosum group G (XPG) and X-ray repair cross complementing group 1 (XRCC1) were associated with the tumor response in non-small cell lung cancer (NSCLC) patients treated with platinum-based chemotherapy in Chinese population. Totally 82 patients with advanced NSCLC were routinely treated with cisplatin or carboplatin-based chemotherapy, and clinical response was evaluated after 2-3 cycles. And 3D (three dimensions) polyacrylamide gel-based DNA microarray method was used to evaluate the genotypes of XRCC1 194 Arg/Trp, XRCC1 399Arg/Gln, XPG 46His/His and XPG 1104His/Asp in DNA from peripheral lymphocytes. We found that there was a significantly increased chance of treatment response to platinum-based chemotherapy with the XRCC1 194Arg/Trp genotype (odds ratio 0.429; 95% CI 0.137-1.671; P=0.035). The polymorphism of XPG 46His/His was found to be associated with clinical response in NSCLC patients P=0.047, not detected between chemotherapy response and SNPs of XRCC1 399Arg/Gln or XPG 1104His/Asp (P=0.997 0.561, respectively). Our study showed that the polymorphic status of XRCC1 194Arg/Trp might be a predictive marker of treatment response for advanced NSCLC patients and those of XPG His46His was associated with susceptibility of chemotherapy. The 3D polyacrylamide gel-based DNA microarray method was accurate, high-throughput and inexpensive, especially suitable for a large scale of SNP genotyping in population.

Catalytic growth of carbon nanotubes from the internal surface of Fe-loading mesoporous molecular sieves materials

Catalytic formation of carbon nanotubes on Fe-loading hexagonal mesoporous molecular sieve Fe/SiHMS and microporous molecular sieve Fe/NaY has been investigated. TEM pictures, together with the different porous properties of SiHMS and NaY, show us that the carbon nanotubes probably grow inside the channels of Fe/SiHMS and on the external surface of Fe/NaY. The Fe(2p) binding energy (BE) suggests a stronger interaction between SiHMS and the deposited iron oxide species than that between NaY and iron species. The BE of Si, O and Fe elements for Fe/SiHMS vary much before and after the catalytic synthesis of carbon nanotubes, whereas the BE for NaY changes little. The above XPS information can serve as a supplement to TEM analysis.

Orientation of Antibodies on a 3-Aminopropyltriethoxylsilane-Modified Silicon Wafer Surface

An antibody can be specifically oxidized with periodate (NaIO4) on the carbohydrate side chains at its C-terminal. Rabbit anti-hepatitis B surface antigen (anti-HBsAg) IgG antibodies were bound to the silicon wafer surface by covalent bonds between aldehydes generated on the carbohydrate side chains of the antibodies and the reactive amine groups of 3-aminopropyltriethoxylsilane(APTES)-modified silicon wafer surfaces. A control experiment was also performed by direct attachment of antibodies to glutaraldehyde-treated silicon surfaces. Two different coupling antibody strategies were investigated in this paper. Atomic force microscopy was used to observe the orientation of the site-directed and random attachment of rabbit anti-HBsAg IgG antibodies and the conservation of their antigen-binding capacity (AgBC) was assessed using an enzyme immunoassay (EIA).

The Performance of An All-Solid-State Electrochromic Display Consisting of WO3 and TiO2 Films with Polyethylene Oxide Gel Electrolyte

An all-solid-state electrochromic display employing a sputtered WO3 film as the electrochromic layer and sputtered TiO2 film as the counterelectrode with a polyethylene oxide (PEO)-based gel electrolyte has been fabricated. The properties of the WO3 film, TiO2 film and PEO gel electrolyte have been investigated. The conductivity of the PEO gel electrolyte is as high as 2.6 mS/cm at room temperature. The electrochromic display developed by us was found to exhibit excellent electrochromic characteristics.

Study on dissolution mechanism of cortisol and cortisone from hair matrix with liquid chromatography–tandem mass spectrometry

BACKGROUND Hair cortisol has been used as a biomarker of chronic stress. The detected contents of hair cortisol might depend on the incubation duration in solvents for no-milled hair samples with 3-layer structure. However, there was no research on the dissolution mechanism of hair analytes. METHODS After uniform mixture, no-milled hair samples were incubated in methanol and water for the 12 different durations and milled hair was done as comparison. Hair cortisol and cortisone were determined with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). RESULTS The measured concentrations of hair cortisol and cortisone showed ≥2 maxima during the entire incubation in methanol and water from 5 min to 72 h for no-milled hair. Hair cortisol concentration measured by LC-MS/MS was increased with the incubation duration. Conversely, it was not held when hair was powdered prior to the incubation in methanol. CONCLUSIONS Hair cortisol and cortisone were dissolved from hair matrix through the 2-stage or multistage mechanism, which might depend on the hair 3-layer structure and its degree of damage.

Optical and nonlinear optical properties of surface-modified CdS nanoparticles

Abstract Hyper-Rayleigh scattering (HRS) technique was used to measure the first-order hyperpolarizability, β , of CdS nanoparticles with different surface-modifying agents (anion of surfactant bis (2-ethylhexyl) sulfosuccinate, disodium salt (named as AOT-SO 3 − ) and pyridine). The ‘per particle’ (4 nm mean diameter) β -values are estimated to fall in the range of 10 −27 esu, which is among the largest values reported for solution species and the β -value of pyridine-modified CdS is 1.4-times smaller than AOT-SO 3 − -modified CdS. As a possible explanation, this difference in the β -values is attributed to the weakening of the two-photon resonance enhancement effect in the pyridine-modified CdS nanoparticles due to the σ-donation formation of pyridine to the Cd 2+ -rich surface of CdS. These were demonstrated by two-photon fluorescence measurements, one-photon fluorescence quenching experiments and photolysis experiments.

Lanthanide ion induced formation of stripes domain structure in phospholipid Langmuir-Blodgett monolayer film observed by atomic force microscopy

Long-range ordered stripes domain structures were observed in Dipalmitoylphosphatidylcholine (DPPC) Langmuir-Blodgett monolayer film which was spread on the subphase of lanthanide ion (Eu3+) solution and transferred to a freshly cleaved mica substrate by vertical deposition. This novel phenomenon was discussed in terms of the competitive interaction of dipole-dipole and electrostatic interactions of the DPPC molecules combined with lanthanide ions with those DPPC molecules free of lanthanide ions.

A novel structure observed on the phospholipid Langmuir-Blodgett bilayer by atomic force microscopy

A novel structure consisting of roundish objects formed on dimyristoyl-phosphatidylcholine (DMPC) Langmuir-Blodgett bilayer films was observed by atomic force microscopy (AFM). Such a structure may have resulted from the collapse of DMPC liposomes formed at the interface of water/organic phase when they have lost the water support.

Molecular positional order in a dipalmitoylphosphatidic acid Langmuir–Blodgett monolayer by atomic force microscopy

Abstract Langmuir–Blodgett monolayers of dipalmitoylphosphatidic acid were studied by using atomic force microscopy on the large- and nano-scale. A molecularly resolved image was achieved at high surface pressure. The monolayer shows a dramatic long-range orientational and positional ordering of molecular organization of aliphatic tails. The ordered molecular arrangement of aliphatic tails may result from the strong intermolecular hydrogen bonding interactions between adjacent phosphate groups in the polar region.

Ti-based oxide thin films deposited from solution on self-assembly monolayer

Abstract Mercaptopropyltrimethoxysilane-bearing mercapto groups were used to form self-assembly monolayers (SAMs) on glass substrates. The terminal groups (-SH) of SAMs were oxidized into sulphonate groups (-SO 3 H). The role of the sulphonate groups is to augment hydrolysis in the bulk solution by providing anionic reaction centers for oxide formation. The deposition of titanium oxide thin films in varied acidity of TiCl 4 solution has a different yield. Several titanium oxidation states exist in the deposited thin films.