Lubing Zhou
Johnson & Johnson
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Publication
Featured researches published by Lubing Zhou.
Methods in Enzymology | 1997
Lubing Zhou; Gail Otulakowski; Catherine Y. Lau
Publisher Summary The use of linked reverse transcription–polymerase chain reactions (RT–PCR) to quantitate specific mRNAs is rapidly gaining favor over Northern blot or RNase protection analyses due to its convenience, speed, and sensitivity. However, accurate quantitative results require careful design of proper controls. The system described in this chapter uses a synthetic RNA generated by in vitro transcription from the cDNA of human CRABP-II, with a small deletion in the coding region. Using identical primers, the test sample and internal control amplify competitively in each reaction tube. This strategy avoids problems due to tube-to-tube variations, unequal amplification efficiencies, and the plateau effect that arise with external controls or unrelated internal controls. A titration system is used to quantitate CRABP-II mRNA accurately. A serial dilution of the sample RNA, together with a fixed amount of internal control RNA, is co-reverse transcribed by a CRABP-II-specific primer. An aliquot of each RT reaction is subsequently amplified by PCR. Because the target and control cDNAs amplify with identical efficiencies, the ratio of the two products is cycle number independent, and the products can be allowed to accumulate to high levels. Following polyacrylamide gel electrophoresis (PAGE), the two products are visualized by ethidium bromide staining.
Thrombosis and Haemostasis | 1996
Andrew L. Darrow; Wai-Ping Fung-Leung; Richard D. Ye; Rosemary J. Santulli; Wai-man Cheung; Carol Burns; Bruce P. Damiano; Lubing Zhou; Charlotte Keenan; Per A. Peterson; Patricia Andrade-Gordon
European Journal of Immunology | 1996
Lubing Zhou; Barbara L. Pope; Erika Chourmouzis; Wai-Ping Fung-Leung; Catherine Y. Lau
Archive | 1997
Wai-Ping Leung; Lars Karlsson; Lubing Zhou; Per A. Peterson
Archive | 1997
Wai-Ping Leung; Lars Karlsson; Lubing Zhou; Per A. Peterson
Archive | 1999
Wai-ping Leung; Patricia Andrade-Gordon; Lubing Zhou
Archive | 1998
Lars Karlsson; Wai-Ping Leung; Per A. Peterson; Lubing Zhou
Archive | 1997
Wai-Ping Leung; Lars Karlsson; Lubing Zhou; A Peterson
Archive | 1997
Wai-Ping Leung; Lars Karlsson; Lubing Zhou; A Peterson
Archive | 1997
Lars Karlsson; Wai-Ping Leung; A. Per Rancho Santa Fe Peterson; Lubing Zhou