Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Lucia Gardossi is active.

Publication


Featured researches published by Lucia Gardossi.


Chemical Society Reviews | 2013

Efficient immobilisation of industrial biocatalysts: criteria and constraints for the selection of organic polymeric carriers and immobilisation methods

Sara Cantone; Valerio Ferrario; Livia Corici; Cynthia Ebert; Diana Fattor; Patrizia Spizzo; Lucia Gardossi

Efficient immobilisation protocols are the result of perfect matching of factors depending on the enzyme, the process and the support for immobilisation. Physical-chemical phenomena, such as partition, solvation and diffusion, strongly affect the efficiency of the biocatalyst in each specific reaction system. Therefore, tailored solutions must be developed for each specific process of interest. Indeed, direct investigation of what occurs at the molecular level in a reaction catalysed by an immobilised enzyme is a quite formidable task and observed differences in the performance of immobilised biocatalysts must be interpreted very carefully. In any study dealing with enzyme immobilisation the prerequisite is the rigorous planning and reporting of experiments, being aware of the complexity of these multi-phase systems.


Proceedings of the National Academy of Sciences of the United States of America | 2009

Dendritic trafficking of BDNF mRNA is mediated by translin and blocked by the G196A (Val66Met) mutation

Cristina Chiaruttini; Annalisa Vicario; Zili Li; Gabriele Baj; Paolo Braiuca; Yaohong Wu; Frank Lee; Lucia Gardossi; Jay M. Baraban; Enrico Tongiorgi

Alternatively spliced brain-derived neurotrophic factor (BDNF) transcripts are targeted to distinct cellular compartments in neurons but the mechanisms underlying this sorting are unknown. Although only some BDNF isoforms are targeted to dendrites, we have found that the coding region common to all BDNF transcripts contains a constitutively active dendritic targeting signal and that this signal is suppressed in transcripts containing exons 1 or 4, which are restricted to the cell soma and proximal dendrites. This dendritic targeting signal is mediated by translin, an RNA-binding protein implicated in RNA trafficking, and is disrupted by the G196A mutation associated with memory deficits and psychiatric disorders. Molecular modeling and mutational studies indicate that the G196A mutation blocks dendritic targeting of BDNF mRNA by disrupting its interaction with translin. These findings implicate abnormal dendritic trafficking of BDNF mRNA in the pathophysiology of neuropsychiatric disorders linked to the G196A mutation.


Trends in Biotechnology | 2010

Guidelines for reporting of biocatalytic reactions.

Lucia Gardossi; Poul Børge Rosenius Poulsen; Antonio Ballesteros; Karl Hult; Vytas K. Švedas; Đurđa Vasić-Rački; Giacomo Carrea; Anders Magnusson; Andreas Schmid; Roland Wohlgemuth; Peter J. Halling

Enzymes and whole cells are being increasingly applied in research and industry, but the adoption of biocatalysis relies strongly on useful scientific literature. Unfortunately, too many published papers lack essential information needed to reproduce and understand the results. Here, members of the scientific committee of the European Federation of Biotechnology Section on Applied Biocatalysis (ESAB) provide practical guidelines for reporting experiments. The document embraces the recommendations of the STRENDA initiative (Standards for Reporting Enzymology Data) in the context of pure enzymology and provides further guidelines and explanations on topics of crucial relevance for biocatalysis. In particular, guidelines are given on issues such as the selectivity, specificity, productivity and stability of biocatalysts, as well as on methodological problems related to reactions in multiphase systems. We believe that adoption and use of these guidelines could greatly increase the value and impact of published work in biocatalysis, and hence promote the further growth of applications.


Trends in Biotechnology | 2016

The Closure of the Cycle: Enzymatic Synthesis and Functionalization of Bio-Based Polyesters.

Alessandro Pellis; Enrique Herrero Acero; Valerio Ferrario; Doris Ribitsch; Georg M. Guebitz; Lucia Gardossi

The polymer industry is under pressure to mitigate the environmental cost of petrol-based plastics. Biotechnologies contribute to the gradual replacement of petrol-based chemistry and the development of new renewable products, leading to the closure of carbon circle. An array of bio-based building blocks is already available on an industrial scale and is boosting the development of new generations of sustainable and functionally competitive polymers, such as polylactic acid (PLA). Biocatalysts add higher value to bio-based polymers by catalyzing not only their selective modification, but also their synthesis under mild and controlled conditions. The ultimate aim is the introduction of chemical functionalities on the surface of the polymer while retaining its bulk properties, thus enlarging the spectrum of advanced applications.


Biotechnology Progress | 2009

Endo- and exo-inulinases: enzyme-substrate interaction and rational immobilization.

Alessandra Basso; Patrizia Spizzo; Valerio Ferrario; Lorena Knapic; Nina Savko; Paolo Braiuca; Cynthia Ebert; Emanuele Ricca; Vincenza Calabrò; Lucia Gardossi

Three‐dimensional models of exoinulinase from Bacillus stearothermophilus and endoinulinase from Aspergillus niger were built up by means of homology modeling. The crystal structure of exoinulinase from Aspergillus awamori was used as a template, which is the sole structure of inulinase resolved so far. Docking and molecular dynamics simulations were performed to investigate the differences between the two inulinases in terms of substrate selectivity. The analysis of the structural differences between the two inulinases provided the basis for the explanation of their different regio‐selectivity and for the understanding of enzyme‐substrate interactions. Surface analysis was performed to point out structural features that can affect the efficiency of enzymes also after immobilization. The computational analysis of the three‐dimensional models proved to be an effective tool for acquiring information and allowed to formulate an optimal immobilized biocatalyst even more active that the native one, thus enabling the full exploitation of the catalytic potential of these enzymes.


Green Chemistry | 2015

Towards feasible and scalable solvent-free enzymatic polycondensations: integrating robust biocatalysts with thin film reactions

Alessandro Pellis; Livia Corici; Loris Sinigoi; Nicola D'Amelio; Diana Fattor; Valerio Ferrario; Cynthia Ebert; Lucia Gardossi

There is an enormous potential for synthesizing novel bio-based functionalized polyesters under environmentally benign conditions by exploiting the catalytic efficiency and selectivity of enzymes. Despite the wide number of studies addressing in vitro enzymatic polycondensation, insufficient progress has been documented in the last two decades towards the preparative and industrial application of this methodology. The present study analyses bottlenecks hampering the practical applicability of enzymatic polycondensation that have been most often neglected in the past, with a specific focus on solvent-free processes. Data here presented elucidate how classical approaches for enzyme immobilization combined with batch reactor configuration translate into insufficient mass transfer as well as limited recyclability of the biocatalyst. In order to overcome such bottlenecks, the present study proposes thin-film processes employing robust covalently immobilized lipases. The strategy was validated experimentally by carrying out the solvent-free polycondensation of esters of adipic and itaconic acids. The results open new perspectives for enlarging the applicability of biocatalysts in other viscous and solvent-free syntheses.


Tetrahedron-asymmetry | 2000

d-Phenylglycine and d-4-hydroxyphenylglycine methyl esters via penicillin G acylase catalysed resolution in organic solvents

Alessandra Basso; Paolo Braiuca; Luigi De Martin; Cynthia Ebert; Lucia Gardossi; Paolo Linda

Abstract Penicillin G acylase in organic solvents catalyses specifically the acylation of the l -enantiomers of methyl esters of phenylglycine and 4-hydroxyphenylglycine. Hydrolytic reactions are prevented by controlling the water activity of the system and no excess of acylating agent is required. The process leads to the facile isolation of the enantiomerically pure d -enantiomer, which is of practical use for the preparation of β-lactam antibiotics. Electrospray mass spectroscopy has been applied to the study of the enantioselectivity of the enzyme.


Tetrahedron Letters | 2002

Synthesis of octyl glucopyranoside by almond β-glucosidase adsorbed onto Celite R-640®

Alessandra Basso; Amélie Ducret; Lucia Gardossi; Robert Lortie

Abstract The synthesis of octyl glucoside from p -nitrophenyl glucopyranoside ( p -NPG) and 1-octanol was carried out with almond β-glucosidase adsorbed onto Celite R-640®. The influence of the amount of water added to the system as well as the addition of co-solvents have been studied. The presence of small percentages of DMF has a strong denaturing effect on the enzyme adsorbed onto Celite R-640®. Denaturation is less pronounced with other co-solvents such as acetonitrile, 2-methyl-2-butanol or ethyl acetate.


Frontiers in Neuroscience | 2013

Toward a unified biological hypothesis for the BDNF Val66Met-associated memory deficits in humans: a model of impaired dendritic mRNA trafficking

Gabriele Baj; Davide Carlino; Lucia Gardossi; Enrico Tongiorgi

Brain-derived neurotrophic factor (BDNF) represents promotesa key molecule for the survival and differentiation of specific populations of neurons in the central nervous system. BDNF also regulates plasticity-related processes underlying memory and learning. A common single nucleotide polymorphism (SNP) rs6265 has been identified on the coding sequence of human BDNF located at 11p13. The SNP rs6265 is a single base mutation with an adenine instead of a guanine at position 196 (G196A), resulting in the amino acid substitution Val66Met. This polymorphism only exists in humans and has been associated with a plethora of effects ranging from molecular, cellular and brain structural modifications in association with deficits in social and cognitive functions. To date, the literature on Val66Met polymorphism describes a complex and often conflicting pattern of effects. In this review, we attempt to provide a unifying model of the Val66Met effects. We discuss the clinical evidence of the association between Val66Met and memory deficits, as well as the molecular mechanisms involved including the reduced transport of BDNF mRNA to the dendrites as well as the reduced processing and secretion of BDNF protein through the regulated secretory pathway.


Tetrahedron Letters | 1996

CONTROL OF ENZYME HYDRATION IN PENICILLIN AMIDASE CATALYSED SYNTHESIS OF AMIDE BOND

Cynthia Ebert; Lucia Gardossi; Paolo Linda

Penicillin amidase catalyses the synthesis of amide bond in very high yield (>98%), using equimolar concentrations of the amine and the phenylacetic components. In situ hydrated phosphates were employed for controlling the water activity in a benzene/water system (97:3 v/v), where the water is taken up by the salt with formation of the hydrated species.

Collaboration


Dive into the Lucia Gardossi's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge