Luciana Dalcin

Cytoskeleton structure, pattern of mitochondrial activity and ultrastructure of frozen or vitrified sheep embryos☆

Even though sheep embryo cryopreservation is a commonly used procedure the survival and pregnancy outcomes can vary greatly. This study investigated whether cryopreservation was causing subtle changes in ultrastructure, mitochondrial activity or cytoskeletal integrity. Sheep embryos were either slow cooled in 1.5 M EG (n = 22), or vitrified in 20% EG + 20% DMSO with 0.5 M sucrose in Open Pulled Straws (OPS) (n = 24). One hour after warming the cryopreserved embryos differed from control embryos in that they had no mitochondrial activity combined with cytoskeletal disorganization and large vesicles. Vitrified embryos also showed many points of cytoskeleton disruption. Ultrastructural alterations resulting from actin filaments disorganization were observed in both cryopreserved groups. This includes areas presenting no cytoplasmic organelles, Golgi complex located far from the nucleus and a decrease of specialized intercellular junctions. Additionally, large vesicles were observed in vitrified morulae and early blastocysts. The alterations after cryopreservation were proportional to embryo quality as assessed using the stereomicroscope. Even in the absence of mitochondrial activity, grade I and II cryopreserved embryos contained mitochondria with normal ultrastructure. Embryos classified as grade I or II in the stereomicroscope revealed mild ultrastructural alterations, meaning that this tool is efficient to evaluate embryos after cryopreservation.

Performance and carcass traits of Santa Inês lambs finished with different sources of forage

The objective of this study was to evaluate performance and biometrics of lambs fed different sources of forage. Twenty-four six-month-old Santa Ines female lambs were randomly allocated to four experimental diets and housed in individual stalls. They weighed on average 26.35±0.20 kg. The diets were coast cross hay (HAY), cassava hay (CAS), dehydrated by-product of pea crop (PEA) and saccharin (SAC). The diets were formulated with the same amount of protein and energy with fixed levels of forage (60%) and concentrate (40%). Adaptation to the diet took 7 days, with 45 days on experiment. Weights and biometric measurements were obtained every fortnight and feed intake three times a week. Rights half-carcasses were weighed and sectioned into retail cuts, rib, loin, shoulder, belly, neck and leg, which were weighed individually. Weight gain in lambs was significantly different between diets, with those fed saccharin gaining more. Overall, treatments did not significantly affect biometric measurements. There was a significant difference for feed intake and live weight at the end of the experiment. Animals fed PEA and SAC showed the best results. Average positive correlations were found between biometric measurements and live weight. Treatment PEA had heavier hot (14.36 kg) and cold (14.01 kg) carcass weights than the other groups. Hot carcass kill-out was higher for animals fed PEA as well as ham weight, belly, neck and heart girth. The weight of the abdominal viscera (%) for lambs fed CAS was greater than those fed SAC and PEA. The weights of the thoracic viscera as well as the liver, for lambs fed PEA were higher. The by-product of pea yielded best results, followed by saccharin, and can replace traditional forage sources in the region, providing similar results in terms of cuts and body components. These could be an alternative for feeding sheep in the dry season. The substitution of forages using by-product of pea and saccharin led to improved productivity over the coast-cross hay available in the Federal District - Brazil.

Avaliação clínica e molecular de cães com erliquiose

The canine monocytic ehrlichiosis is a cosmopolitan disease, caused by Ehrlichia canis, transmitted by ticks Rhipicephalus sanguineus that has been frequently diagnosed in dogs throughout the country. This study aimed to investigate the cytological and molecular Ehrlichia infection in 195 dogs examined at the University Veterinary Hospital of Mato Grosso, by analyzing the clinical and laboratory findings. In 48 dogs with positive cytology for Ehrlichia sp it was possible to detect the diversity of signs, with predominance statistically significant of pallor of mucous membranes (P≤0.05) as well as several hematological findings, occurring anemia, leukopenia and thrombocytopenia, or increased as normal cells. There was increased of plasma proteins, with hyperglobulinemia, however without any significant difference (P≥0.05), although this finding is common in that infection. Through the nested PCR technique it was possible to confirm the infection by E. canis in the city of Cuiaba.