Luciana Keiko Hatamoto-Zervoudakis

Supplementation frequency and proteins sources for growing of steers in pasture during the dry season: productive and economical performance

Two experiments were carried out with the objective of evaluating the effects of the frequency of supplementation with two proteins sources on productive and economical performance (experiment 1) and the nutrients digestibility of steers in supplemented pasture during the dry season over a year. It was used twenty weaned zebu calves at the age of 10 months and initial live weight (LW) of 208.43 kg, distributed in four paddocks of Brachiaria brizantha cv. Marandu, each one with 1.6 ha. Both experiments were structured in 2 × 2 factorial arrangement with two supplements (with soybean meal and high energy cottonseed meal). There was no protein sources × supplementation frequency interaction for any of the economical and productive performance traits. The protein sources didn’t affect the daily average gain, but the frequency of supply affected performance, which was better in the animals under supplementation at three times a week (0.670 kg /day). The highest economic return was obtained with the offer of supplement with cottonseed meal three times a week. The frequency of supplementation affect the nutrients intake, while the protein source of the supplement had effect only on the ethereal extract intake, which was higher in the animals that consumed the supplement with cottonseed meal. The supplement with high energy cottonseed meal provided higher digestibility of diet crude protein. The supply of supplement three times a week, not only reduces the costs of supplementation, but it possibilities a higher performance than the one obtained with daily supplementation. The use of higher energy cottonseed meal in multiple supplements replacing soybean meal is satisfactory mainly under economical point of view.

Substituição do farelo de soja por farelo de algodão de alta energia em dietas para vacas leiteiras em produção: consumo, digestibilidade dos nutrientes, balanço de nitrogênio e produção leiteira

The effect of replacing increasing levels of high-energy cottonseed meal (0, 8.7, 17.4, 26.1 and 34,8% DM) with soybean meal in concentrate for cows in the final lactation third on intake, nutrient digestibility, nitrogen efficiency and milk yield was assessed. Five crossbred 5/8 Holstein × 3/8 Gir cows were allotted to a 5 × 5 Latin square design, with five 18-day periods (eight days for adaptation and 10 days to determine intake and collect samples). The diets were formulated to have similar protein contents (14% CP), with 60% corn silage and 40% on the DM. There was no effect of including high energy cottonseed meal on the intakes of DM, organic matter (OM), crude protein (CP), total carbohydrates (TCHO) and total digestible nutrients). The EE intake increased with levels of 26.1 and 34.8% of high energy cottonseed meal inclusion, while the non-fibrous carbohydrate (NFC) intake was higher for levels of 0, 8.7 and 26.1%. The digestibility coefficients of the nutrients did not differ among the high energy cottonseed meal levels experimental diets. Replacing soybean hulls levels with high energy cottonseed meal did not influence the nitrogen use efficiency or balance, or the milk urea nitrogen content (MUN) and blood urea nitrogen (BUN). Average milk yield (14.03 kg/day) and milk yield corrected for 3.5% fat milk fat (14.68 kg/day) were not influenced by the different levels of high-energy cottonseed meal in the diet and there was no difference for feed efficiency. High-energy cottonseed meal presents potential for replacing soybean meal in the diet of cows with 15 kg/day production capacity.

Uso do acetato de melengestrol após protocolos de inseminação artificial em tempo fixo em vacas Nelore multíparas

Objective was to evaluate the effects of supply 2.28 g MGA® Premix between the 13th and 17th day after fixed-time artificial insemination (TFAI), on pregnancy rate of multiparous Nellore cows. It used 154 cows, divided randomly into two lots, Treatments 1, n = 55 and Treatments 2, n = 99. The treatments 1 received only one mineral supplement and treatments 2 received the same batch mineral supplement added 2.28g MGA® Premix/animal/day. We used descriptive statistics for frequency table, on program SAS, by the interaction of supply MGA® Premix with the rate of pregnancy. The pregnancy rate was higher for cows supplemented MGA® Premix evaluated at 43 days of pregnancy. Using TFAI after MGA® Premix increases the rate of embryo survival and rate of pregnancy.

Suplementação infrequente e fontes proteicas para recria de bovinos em pastejo no período seco: parâmetros nutricionais

The objective of this study was to evaluate the effects of protein sources and frequency of supplement offer on the intake and nutrient digestibility, on the concentrations of ammonia nitrogen (N-NH3), on rumen pH and on serum urea nitrogen concentrations in steers grazing pasture of Brachiaria brizantha cv. Marandu during the dry season. Five crossbred Zebu × Angus steers with initial live weight of 295 kg, castrated and fitted with rumen canullae were used in a 5 × 5 Latin square design. It was evaluated two isoprotein supplements, one with soybean meal (SM) and the other with high energy cottonseed meal, offered at two frequencies: every day (7 times a week), or 3 times a week (on Mondays, Wednesdays and Fridays); and a mineral mixture (MM). The supplementation increased the intake of CP, of ether extract (EE) and non-fibrous carbohydrates (NFC) in kg/day. The supplement with cottonseed meal increased the intake of ether extract. There was a positive effect of the three times a week supplementation on the intakes of total dry matter, of forage, of organic matter of forage, total carbohydrates (TC) and NDF. The supplementation reduced the digestibility of the NDF and increased the digestibility of the CP. The supplement with cottonseed meal reduced digestibility of NDF and total carbohydrates. Three times a week supplementation did not affect digestibility of the nutrients neither the rumen pH. Supplement intake increases concentrations of N-NH3, especially if it is carried out 3 times a week. Concentrations of urea nitrogen in the serum of growing steers increase.

Suitable strategy to improve nitrogen utilization and reduce the environmental impact of Nellore bulls supplemented on tropical pasture.

Expansion of the biodiesel industry has increased the crude glycerin (CG) supply. Crude glycerin has the potential of replacing corn in ruminant diets because the glycerol can be converted to glucose in the liver of ruminants, providing energy for cellular metabolism. The objective was to evaluate the effects of CG with urea, soybean meal, cottonseed meal, and corn gluten feed, respectively, on intake, digestibility, microbial protein yield, and efficiency of N utilization. Five Nellore bulls (initial BW of 448 kg [SD 14]) grazing tropical pasture were used in a 5 × 5 Latin square design. The supplements were control (no supplementation; only free-choice mineral mixture ad libitum), CG with urea (CG-Urea), CG with soybean meal, CG with cottonseed meal, and CG with corn gluten. Crude glycerin was used in all supplements to replace corn (15% of DM supplement). There were differences between CG-Urea and other supplements with regard to intake of DM (% of BW and total; < 0.01), OM ( < 0.01), CP ( < 0.01), and TDN ( < 0.01). The digestibility of CP was greater ( = 0.04) for animals supplemented with CG-Urea than for those fed other supplements. Animals supplemented with CG-Urea showed greater N intake ( < 0.01) and N ammonia ( = 0.04) than those supplemented with other treatments. Nitrogen retained (g/d) was not affected by protein source but was greater for cattle fed a protein supplement compared with cattle fed the control supplement ( < 0.01). Supplementing the animals with protein sources increased ( = 0.02) the daily production of rumen microbial nitrogen (g/d) compared with the control group. Microbial protein (g/d) was lesser for the control than for protein sources ( = 0.02). However, when expressed relative to TDN ( = 0.35) and CP ( = 0.82), there were no differences across treatments. Crude protein intake per digestible OM intake (g CP/kg digestible OM intake) was greater for animals fed protein sources compared with animals fed control supplements ( < 0.01). Based on nutrients intake and microbial protein yield, CG-Urea supplement has a greater feeding value compared with other protein sources. Crude glycerin, when used to replace corn in 15% of DM supplement, may be effective to improved N utilization and microbial protein yield in rumen of Nellore bulls grazing cv. Marandu.

Free gossypol supplementation frequency and reproductive toxicity in young bulls

The aim of the present study was to analyze seminal quality of young bulls subjected to different frequencies of gossypol supplementation. Forty-eight Nellore bulls, with 19 months of age and weighing 357.8 ± 7.2 kg, were used in this study. Animals were fed with 10.5 kg of standard supplement containing free-gossypol from whole cottonseed (WCS) at the following frequency: 3x/week (G3x), 5x/week (G5x) or 7x/week (G7x - Control). Additionally, a negative control was provided, and the treated animals received only mineral supplement (MM) ad libtum. The experiment lasted for 84 days and semen was collected at the beginning and at the end for analysis and cryopreservation. Fresh semen was used for initial analysis and plasma membrane integrity and sperm morphology were also determined. General motility using computer assisted sperm analysis (CASA), plasma and acrosomal membranes integrity, mitochondrial activity, and induced oxidative stress were assessed in post-thawed semen. The study design was completely randomized. Parametric data were analyzed by ANOVA and non-parametric data by the Wilcoxon test, using the statistical program SAS. Level of significance was set at 5%. Supplementation with WCS, regardless the frequency, increased total (P = .009) and head (P = .005) defects in comparison to animals receiving only forage and mineral supplement. Infrequent supplementation, particularly 5 times in the week (G5X), increased head (P = .026) and midpiece (P = .014) abnormalities. Sperm motility in fresh semen was lower in animals that received daily supplementation than those supplemented on alternate days (P = .021). Additionally, animals supplemented daily showed lower percentage of spermatozoa with intact acrosome compared to those supplemented on alternate days (P = .005). Thus, regardless the frequency of supplementation, free-gossypol supplementation affects sperm quality. Although the amount of free gossypol supplied weekly was the same among treatments, daily supplementation compromised sperm kinetics, differently from infrequent supplementation that led to sperm defects developed during spermatogenesis.

Características seminais e de membrana espermática em touros suplementados com tocoferol

This study had objective to evaluate the integrity of the plasma membrane and semen quality of bulls supplemented with tocopherol. Bulls were used 16 with an average age of 24 months and average weight of 462.2 kg in two treatments: control group (CG) and group supplemented with tocopherol (GE-400 IU of tocoferol/animal / day) added to the concentrate supplement. The groups were kept in pasture supplemented with 4.5 kg / animal / day concentrate. Tocopherol supplementation was provided for 60 days. 4 semen samples were carried out: on days 0 , 30, 60 and 15 days after the end of supplementation. Semen was collected by electroejaculation , it has measured up the physical and morphological sperm characteristics. To assess the integrity of membrane used the hyposmotic test, eosin / nigrosine, Pope and trypan blue. The experiment was conducted in a completely randomized design with repeated measurements over time. Data were analyzed using ANOVA and SNK test with a 5% significance level with assistance from the SAS statistical program. It found effect of supplementation force (p = 0.0183) (CG = 2.71 ± 0.095 and GE = 2.23 ± 0.16) ;. There were no differences (p> 0.05) for the other variables: motility, concentration, morphology, acrosome integrity, sperm viability and membrane integrity. With the results obtained in the experimental conditions of this study, it is concluded that oral supplementation with tocopherol 400 IU / day showed no improvement over the seminal quality or integrity of the sperm membrane Bulls.

Início da puberdade de cordeiros provenientes de ovelhas deslanadas submetidas à restrição calórica ou proteica

This study was carried out to evaluate if caloric or protein restriction in lactation ewe affect puberty beginning of its lambs. Had been used 21 lambs, two females and five males per type of treatment. During 60 days the mothers received the treatment for which its lamb was sort out: Control Treatment (TC, maintenance), Energy Restriction Treatment (TRC, ½ of the energy of the TC) and Protein Restriction Treatment (TRP, 1/3 of the protein of the TC). The lambs were weaned and received a diet composed by corn silage and concentrate, weighed and evaluated fortnightly. In males, the ejaculate was collected, scrotal circumference and testicular size measured. In females, it was realized ultrasound to measure the ovaries and verify the corpus luteum presence. For males was considered in puberty if produced ejaculation with at least: motility of 50%, sperm concentration of 50 x 106espermatozoa/mL and maximum of 50% total sperm abnormalities, and females if exhibit corpus luteum. An interaction was found between evaluation period and treatment for scrotum circumference (p=0.0019), testicular volume (p=0.0025) sperm motility (p 0.05) were found about follicles characteristics, however that was difference in the presence of corpous luteum (p=0.0179). The TC females showed corpus luteum from the 10th week, TRC 11th and 13th for TRP. It was concluded that mother protein restriction during the lactation delays the beginning of the puberty of its lambs.