Luciane de Albuquerque

Avaliação do endotélio corneano suíno por microscopia eletrônica de varredura após aplicação de azul brilhante a 0,05% na câmara anterior: estudo in vitro

Com o objetivo de avaliar as alteracoes ultraestruturais no endotelio corneano suino induzidas pela aplicacao intracameral do corante azul brilhante 0,05%, vinte corneas suinas foram separadas em dois grupos: olhos direitos (grupo controle) e olhos esquerdos (grupo experimental). Inicialmente todos os bulbos oculares foram avaliados por microscopia especular. As corneas dos bulbos oculares do grupo controle foram excisadas, enquanto nos bulbos oculares do grupo experimental foram injetados 0,2ml do corante vital azul brilhante 0,05% na câmara anterior e mantido por um minuto. Apos esse periodo a câmara anterior foi lavada com solucao salina balanceada e as corneas foram excisadas. Todas as corneas excisadas foram avaliadas por microscopia eletronica de varredura para verificar a ocorrencia possiveis alteracoes no endotelio corneano causadas pelo corante. Nao foram observadas diferencas ultraestruturais durante a avaliacao endotelial por microscopia eletronica de varredura das corneas do grupo controle e experimental apos a aplicacao do azul brilhante 0,05% na câmara anterior. O corante azul brilhante na concentracao 0,05% nao causou danos endoteliais apos seu uso intracameral em suinos e pode ser uma alternativa segura para a coloracao da capsula anterior da lente na cirurgia de catarata.

Intraocular pressure measurement in sheep using an applanation tonometer

The purpose of this study was to evaluate and establish the mean values of IOP in healthy adult sheep using an applanation tonometer. Information on age, sex, and breed was obtained for all animals included in this study . Twenty five healthy sheep (Ovis aries), of the same breed (Texel), male or female, with three years of age, received an ophthalmic examination in both eyes, including pupillary reflexes, Schirmer tear test, slit lamp biomicroscopy , and fluorescein staining. For all ophthalmic testing, animals were gently physically restrained, with no pressure in the jugular area and the eyelids were carefully open. IOP was measured by applanation tonometry (Tonopen XL). The same examiner performed the tonometry; measurements were taken three times for each eye, and their average was recorded as the IOP of the animal. Statistical analysis was performed using paired t-test and values of P < 0.05 were considered significant. The mean intraocular pressure in the whole group of 50 eyes was of 16.36 +/- 2.19 mm Hg. The mean (SD) IOP in the right eye was of 15.96 +/- 2.02 mm Hg, while the mean (SD) IOP in the left eye was of 16.76 +/- 2.32 mm Hg. Significant differences in IOP were not found between right and left eyes. The applanation tonometer was adequate for measuring the intraocular pressure in sheep. Reference data will assist in diagnosing testing for ophthalmic disease in sheep, as 1 well as promote further studies in this area.