Luidmila A. Pestryakova

Genetic data from algae sedimentary DNA reflect the influence of environment over geography

Genetic investigations on eukaryotic plankton confirmed the existence of modern biogeographic patterns, but analyses of palaeoecological data exploring the temporal variability of these patterns have rarely been presented. Ancient sedimentary DNA proved suitable for investigations of past assemblage turnover in the course of environmental change, but genetic relatedness of the identified lineages has not yet been undertaken. Here, we investigate the relatedness of diatom lineages in Siberian lakes along environmental gradients (i.e. across treeline transects), over geographic distance and through time (i.e. the last 7000 years) using modern and ancient sedimentary DNA. Our results indicate that closely-related Staurosira lineages occur in similar environments and less-related lineages in dissimilar environments, in our case different vegetation and co-varying climatic and limnic variables across treeline transects. Thus our study reveals that environmental conditions rather than geographic distance is reflected by diatom-relatedness patterns in space and time. We tentatively speculate that the detected relatedness pattern in Staurosira across the treeline could be a result of adaptation to diverse environmental conditions across the arctic boreal treeline, however, a geographically-driven divergence and subsequent repopulation of ecologically different habitats might also be a potential explanation for the observed pattern.

Vegetation and lake changes on the southern Taymyr peninsula, northern Siberia, during the last 300 years inferred from pollen and Pediastrum green algae records

Siberian arctic vegetation and lake water communities, known for their temperature dependence, are expected to be particularly impacted by recent climate change and high warming rates. However, decadal information on the nature and strength of recent vegetation change and its time lag to climate signals are rare. In this study, we present a 210Pb/137Cs dated pollen and Pediastrum species record from a unnamed lake in the south of the Taymyr peninsula covering the period from AD 1706 to 2011. Thirty-nine palynomorphs and 10 morphotypes of Pediastrum species were studied to assess changes in vegetation and lake conditions as probable responses to climate change. We compared the pollen record with Pediastrum species, which we consider to be important proxies of climate changes. Three pollen assemblage zones characterised by Betula nana, Alnus viridis and Larix gmelinii (1706–1808); herbs such as Cyperaceae, Artemisia or Senecio (1808–1879), and higher abundance of Larix pollen (1955–2011) are visible. Also, three Pediastrum assemblage zones show changes of aquatic conditions: higher abundances of Pediastrum boryanum var. brevicorne (1706–1802); medium abundances of P. kawraiskyi and P. integrum (1802–1840 and 1920–1980), indicating cooler conditions while less eutrophic conditions are indicated by P. boryanum, and a mainly balanced composition with only small changes of cold- and warm-adapted Pediastrum species (1965–2011). In general, compositional Pediastrum species turnover is slightly higher than that indicated by pollen data (0.54 vs 0.34 SD), but both are only minor for this treeline location. In conclusion, the relevance of differentiation of Pediastrum species is promising and can give further insights into the relationship between lakes and their surrounding vegetation transferred onto climatic conditions.

A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline

Reliable information on past and present vegetation is important to project future changes, especially for rapidly transitioning areas such as the boreal treeline. To study past vegetation, pollen analysis is common, while current vegetation is usually assessed by field surveys. Application of detailed sedimentary DNA (sedDNA) records has the potential to enhance our understanding of vegetation changes, but studies systematically investigating the power of this proxy are rare to date. This study compares sedDNA metabarcoding and pollen records from surface sediments of 31 lakes along a north–south gradient of increasing forest cover in northern Siberia (Taymyr peninsula) with data from field surveys in the surroundings of the lakes. sedDNA metabarcoding recorded 114 plant taxa, about half of them to species level, while pollen analyses identified 43 taxa, both exceeding the 31 taxa found by vegetation field surveys. Increasing Larix percentages from north to south were consistently recorded by all three methods and principal component analyses based on percentage data of vegetation surveys and DNA sequences separated tundra from forested sites. Comparisons of the ordinations using procrustes and protest analyses show a significant fit among all compared pairs of records. Despite similarities of sedDNA and pollen records, certain idiosyncrasies, such as high percentages of Alnus and Betula in all pollen and high percentages of Salix in all sedDNA spectra, are observable. Our results from the tundra to single‐tree tundra transition zone show that sedDNA analyses perform better than pollen in recording site‐specific richness (i.e., presence/absence of taxa in the vicinity of the lake) and perform as well as pollen in tracing vegetation composition.

The sensitivity of diatom taxa from Yakutian lakes (north-eastern Siberia) to electrical conductivity and other environmental variables

ABSTRACT Relative abundances of 157 diatom taxa from Yakutian lake surface-sediments were investigated for their potential to indicate certain environmental conditions. Data from 206 sites from Arctic, sub-Arctic and boreal environments were included. Redundancy analyses were performed to assess the explanatory power of mean July temperature (TJuly), conductivity, pH, dissolved silica concentration, phosphate concentration, lake depth and vegetation type on diatom species composition. Boosted regression tree analyses were performed to infer the most relevant environmental variables for abundances of individual taxa and weighted average regression was applied to infer their respective optimum and tolerance. Electrical conductivity was best indicated by diatom taxa. In contrast, only few taxa were indicative of Si and water depth. Few taxa were related to specific pH values. Although TJuly explained the highest proportion of variance in the diatom spectra and was, after conductivity, the second-most selected splitting variable, we a priori decided not to present indicator taxa because of the poorly understood relationship between diatom occurrences and TJuly. In total, 92 diatom taxa were reliable indicators of a certain vegetation type or a combination of several types. The high numbers of indicative species for open vegetation sites and for forested sites suggest that the principal turnover is the transition from forest–tundra to northern taiga. Overall, our results reveal that preference ranges of diatom taxa for environmental variables are mostly broad, and the use of indicator taxa for the purposes of environmental reconstruction or environmental monitoring is therefore restricted to marked rather than subtle environmental transitions.

Dispersal distances and migration rates at the arctic treeline in Siberia – a genetic and simulation based study

A strong temperature increase in the Arctic is expected to lead to latitudinal treeline shift. This tundra–taiga turnover would cause a positive vegetation–climate feedback due to albedo decrease. However, reliable estimates of tree migration rates are currently lacking due to the complex processes involved in forest establishment, which depend strongly on seed dispersal. We aim to fill this gap using LAVESI, an individual-based and spatially explicit Larix vegetation simulator. LAVESI was designed to simulate plots within homogeneous forests. Here, we improve the implementation of the seed dispersal function via field-based investigations. We inferred the effective seed dispersal distances of a typical open-forest stand on the southern Taymyr Peninsula (northern central Siberia) from genetic parentage analysis using eight nuclear microsatellite markers. The parentage analysis gives effective seed dispersal distances (median ∼ 10 m) close to the seed parents. A comparison between simulated and observed effective seed dispersal distances reveals an overestimation of recruits close to the releasing tree and a shorter dispersal distance generally. We thus adapted our model and used the newly parameterised version to simulate south-to-north transects; a slow-moving treeline front was revealed. The colonisation of the tundra areas was assisted by occasional long-distance seed dispersal events beyond the treeline area. The treeline (∼ 1 tree ha−1) advanced by ∼ 1.6 m yr−1, whereas the forest line (∼ 100 trees ha−1) advanced by only ∼ 0.6 m yr−1. We conclude that the treeline in northern central Siberia currently lags behind the current strong warming and will continue to lag in the near future.

Late Holocene ice-wedge polygon dynamics in northeastern Siberian coastal lowlands

ABSTRACT Ice-wedge polygons are common features of northeastern Siberian lowland periglacial tundra landscapes. To deduce the formation and alternation of ice-wedge polygons in the Kolyma Delta and in the Indigirka Lowland, we studied shallow cores, up to 1.3 m deep, from polygon center and rim locations. The formation of well-developed low-center polygons with elevated rims and wet centers is shown by the beginning of peat accumulation, increased organic matter contents, and changes in vegetation cover from Poaceae-, Alnus-, and Betula-dominated pollen spectra to dominating Cyperaceae and Botryoccocus presence, and Carex and Drepanocladus revolvens macro-fossils. Tecamoebae data support such a change from wetland to open-water conditions in polygon centers by changes from dominating eurybiontic and sphagnobiontic to hydrobiontic species assemblages. The peat accumulation indicates low-center polygon formation and started between 2380 ± 30 and 1676 ± 32 years before present (BP) in the Kolyma Delta. We recorded an opposite change from open-water to wetland conditions because of rim degradation and consecutive high-center polygon formation in the Indigirka Lowland between 2144 ± 33 and 1632 ± 32 years BP. The late Holocene records of polygon landscape development reveal changes in local hydrology and soil moisture.