Luigi Moroder | Researchain

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Biochimica et Biophysica Acta | 1967

Enzymatic activity of partially synthetic ribonucleases

Armando Marzotto; Fernando Marchiori; Luigi Moroder; Renzo Boni; Lauro Galzigna

Abstract Synthetic peptides, which differ from the natural S-peptide obtained by proteolysis of ribonuclease A (ribonucleate pyrimidinenucleotido-2′-transferase (cyclizing), EC 2.7.7.16), are able to regenerate enzymatic activity when combined with the S-protein. The activity is either depolymerase on yeast nucleic acid or hydrolase on uridine 2′,3′-phosphate. The apparent dissociation constants of the different peptide-protein complexes are calculated, showing the influence of the primary structure on binding. The different behaviour of the reconstituted enzymes is illustrated by pH-activity curves.

Journal of The Chemical Society C: Organic | 1967

Synthesis of peptides analogous to the N-terminal eicosapeptide sequence of ribonuclease A. Part III. A new synthesis of the C-terminal octapeptide 13–20

Fernando Marchiori; Raniero Rocchi; Luigi Moroder; Giorgio Vidali; Ernesto Scoffone

Synthesis is described of the octapeptide corresponding to the 13–20 sequence of the bovine pancreatic ribonuclease A. The pentapeptidebenzyloxycarbonyl-L-seryl-L-threonyl-L-seryl-L-alanyl-L-alanine t-butyl ester was built up by coupling, by the azide procedure, benzyloxycarbonyl-L-seryl-L-threonine hydrazide with L-seryl-L-alanyl-L-alanine t-butyl ester.The subsequent elongation to the octapeptide was carried out stepwise by the p-nitrophenyl ester method. Exposure of the protected peptide to trifluoroacetic acid yielded L-methionyl-L-aspartyl-L-seryl-L-seryl-L-threonyl-L-seryl-L-alanyl-L-alanine.

Journal of the American Chemical Society | 1967

Synthesis of peptide analogs of the N-terminal eicosapeptide sequence of ribonuclease A. VII. Synthesis of Ile8, Orn10- and Ala8, Orn10-eicosapeptides

Ernesto Scoffone; Raniero Rocchi; Fernando Marchiori; Luigi Moroder; Armando Marzotto; Antonio M. Tamburro

Tetrahedron Letters | 1966

New removal conditions of sulfenyl groups in peptide synthesis

Angelo Fontana; Fernando Marchiori; Luigi Moroder; Ernesto Scoffone

Journal of the American Chemical Society | 1969

Synthesis of peptide analogs of the N-terminal eicosapeptide sequence of ribonuclease A. XI. Synthesis and conformational studies of [Orn-10, Nle-13]-S-peptide.

Raniero Rocchi; Angelo Scatturin; Luigi Moroder; Fernando Marchiori; Antonio M. Tamburro; Ernesto Scoffone

Journal of the American Chemical Society | 1969

Synthesis of peptide analogs of the N-terminal eicosapeptide sequence of ribonuclease A. XII. Synthesis of des-Lys1-[Orn10]-, and des-Lys1,Glu2,Thr3-[Orn10]-S-peptides1,2

Luigi Moroder; Fernando Marchiori; Raniero Rocchi; Angelo Fontana; Ernesto Scoffone

Journal of the American Chemical Society | 1969

Synthesis of peptide analogs of the N-terminal eicosapeptide sequence of ribonuclease A. XIII. Synthesis of des-Lys7-[Orn10]- and des-Phe8-[Orn10]-S-peptides

Raniero Rocchi; Fernando Marchiori; Luigi Moroder; Gianfranco Borin; Ernesto Scoffone

Journal of the American Chemical Society | 1968

Synthesis of peptide analogs of the N-terminal eicosapeptide sequence of ribonuclease A. IX. Synthesis of [Serine6,Ornithine10]- and [Proline6,Ornithine10]eicosapeptides

Fernando Marchiori; Raniero Rocchi; Luigi Moroder; Angelo Fontana; Ernesto Scoffone

Journal of the American Chemical Society | 1968

Synthesis of peptide analogs of the N-terminal eicosapeptide sequence of ribonuclease A. 8. Synthesis of (ser4, orn10)-and (ser5, orn10)-eicosapeptides.

Raniero Rocchi; Luigi Moroder; Fernando Marchiori; Ferrarese E; Ernesto Scoffone

International Journal of Peptide and Protein Research | 2009

RELATION BETWEEN STRUCTURE AND FUNCTION IN SOME PARTIALLY SYNTHETIC RIBONUCLEASES S‘. II.*: Kinetic determinations on [Orn10, Glu11], [Orn10, Leu11]- and [Orn10, Lys11]-RNase S'

Fernando Marchiori; Gianfranco Borin; Luigi Moroder; Raniero Rocchi; Ernesto Scoffone

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