Luis A. Hernández Gómez

Smart cities at the forefront of the future internet

Smart cities have been recently pointed out by M2M experts as an emerging market with enormous potential, which is expected to drive the digital economy forward in the coming years. However, most of the current city and urban developments are based on vertical ICT solutions leading to an unsustainable sea of systems and market islands. In this work we discuss how the recent vision of the Future Internet (FI), and its particular components, Internet of Things (IoT) and Internet of Services (IoS), can become building blocks to progress towards a unified urban-scale ICT platform transforming a Smart City into an open innovation platform. Moreover, we present some results of generic implementations based on the ITU-Ts Ubiquitous Sensor Network (USN) model. The referenced platform model fulfills basic principles of open, federated and trusted platforms (FOTs) at two different levels: the infrastructure level (IoT to support the complexity of heterogeneous sensors deployed in urban spaces), and at the service level (IoS as a suit of open and standardized enablers to facilitate the composition of interoperable smart city services). We also discuss the need of infrastructures at the European level for a realistic large-scale experimentally-driven research, and present main principles of the unique-in-the-world experimental test facility under development within the SmartSantander EU project.

Automatic phonetic segmentation

This paper presents the results and conclusions of a thorough study on automatic phonetic segmentation. It starts with a review of the state of the art in this field. Then, it analyzes the most frequently used approach-based on a modified Hidden Markov Model (HMM) phonetic recognizer. For this approach, a statistical correction procedure is proposed to compensate for the systematic errors produced by context-dependent HMMs, and the use of speaker adaptation techniques is considered to increase segmentation precision. Finally, this paper explores the possibility of locally refining the boundaries obtained with the former techniques. A general framework is proposed for the local refinement of boundaries, and the performance of several pattern classification approaches (fuzzy logic, neural networks and Gaussian mixture models) is compared within this framework. The resulting phonetic segmentation scheme was able to increase the performance of a baseline HMM segmentation tool from 27.12%, 79.27%, and 97.75% of automatic boundary marks with errors smaller than 5, 20, and 50 ms, respectively, to 65.86%, 96.01%, and 99.31% in speaker-dependent mode, which is a reasonably good approximation to manual segmentation.

Members of the α-amylase inhibitors family from wheat endosperm are major allergens associated with baker's asthma

We have identified the major antigens or IgE binding components from wheat flour. Thirty‐five sera from patients with bakers asthma were used to analyze the reaction with wheat salt‐soluble proteins. We found a 15 kDa SDS‐PAGE band which reacted with all sera tested. Purified members of the α‐amylase inhibitor family, which are the main components of the 15 kDa band, were recognized by specific IgE when tested with a pool of reactive sera. Immunodetection after two‐dimensional electrophoretic fractionation of crude inhibitor preparations from wheat endosperms also detected several inhibitor subunits as major low‐molecular‐weight allergens.

Protein Cryoprotective Activity of a Cytosolic Small Heat Shock Protein That Accumulates Constitutively in Chestnut Stems and Is Up-Regulated by Low and High Temperatures

Heat shock, and other stresses that cause protein misfolding and aggregation, trigger the accumulation of heat shock proteins (HSPs) in virtually all organisms. Among the HSPs of higher plants, those belonging to the small HSP (sHSP) family remain the least characterized in functional terms. We analyzed the occurrence of sHSPs in vegetative organs of Castanea sativa (sweet chestnut), a temperate woody species that exhibits remarkable freezing tolerance. A constitutive sHSP subject to seasonal periodic changes of abundance was immunodetected in stems. This protein was identified by matrix-assisted laser-desorption ionization time of flight mass spectrometry and internal peptide sequencing as CsHSP17.5, a cytosolic class I sHSP previously described in cotyledons. Expression of the corresponding gene in stems was confirmed through cDNA cloning and reverse transcription-PCR. Stem protein and mRNA profiles indicated that CsHSP17.5 is significantly up-regulated in spring and fall, reaching maximal levels in late summer and, especially, in winter. In addition, cold exposure was found to quickly activate shsp gene expression in both stems and roots of chestnut seedlings kept in growth chambers. Our main finding is that purified CsHSP17.5 is very effective in protecting the cold-labile enzyme lactate dehydrogenase from freeze-induced inactivation (on a molar basis, CsHSP17.5 is about 400 times more effective as cryoprotectant than hen egg-white lysozyme). Consistent with these observations, repeated freezing/thawing did not affect appreciably the chaperone activity of diluted CsHSP17.5 nor its ability to form dodecameric complexes in vitro. Taken together, these results substantiate the hypothesis that sHSPs can play relevant roles in the acquisition of freezing tolerance.

A barley flour inhibitor of insect α‐amylase is a major allergen associated with baker's asthma disease

A barley salt‐soluble protein of 14.5 kDa, which inhibits the α‐amylase from the insect Tenebrio molitor, has been identified as a major IgE‐binding component of sera from bakers asthma patients. The N‐terminal amino acid sequence of this protein indicates that it is a member of a previously described family of α‐amylase/trypsin inhibitors.

Usability evaluation of multi-modal biometric verification systems

As a result of the evolution in the field of biometrics, a new breed of techniques and methods for user identity recognition and verification has appeared based on the recognition and verification of several biometric features considered unique to each individual. Signature and voice characteristics, facial features, and iris and fingerprint patterns have all been used to identify a person or just to verify that the person is who he/she claims to be. Although still relatively new, these new technologies have already reached a level of development that allows its commercialization. However, there is a lack of studies devoted to the evaluation of these technologies from a user-centered perspective. This paper is intended to promote user-centered design and evaluation of biometric technologies. Towards this end, we have developed a platform to perform empirical evaluations of commercial biometric identity verification systems, including fingerprint, voice and signature verification. In this article, we present an initial empirical study in which we evaluate, compare and try to get insights into the factors that are crucial for the usability of these systems.

Purification and in vitro chaperone activity of a class I small heat-shock protein abundant in recalcitrant chestnut seeds.

A 20-kD protein has been purified from cotyledons of recalcitrant (desiccation-sensitive) chestnut (Castanea sativa) seeds, where it accumulates at levels comparable to those of major seed storage proteins. This protein, termed Cs smHSP 1, forms homododecameric complexes under nondenaturing conditions and appears to be homologous to cytosolic class I small heat-shock proteins (smHSPs) from plant sources. In vitro evidence has been obtained that the isolated protein can function as a molecular chaperone: it increases, at stoichiometric levels, the renaturation yields of chemically denatured citrate synthase and also prevents the irreversible thermal inactivation of this enzyme. Although a role in desiccation tolerance has been hypothesized for seed smHSPs, this does not seem to be the case for Cs smHSP 1. We have investigated the presence of immunologically related proteins in orthodox and recalcitrant seeds of 13 woody species. Our results indicate that the presence of Cs smHSP 1-like proteins, even at high levels, is not enough to confer desiccation tolerance, and that the amount of these proteins does not furnish a reliable criterion to identify desiccation-sensitive seeds. Additional proteins or mechanisms appear necessary to keep the viability of orthodox seeds upon shedding.

A major barley allergen associated with baker's asthma disease is a glycosylated monomeric inhibitor of insect α-amylase: cDNA cloning and chromosomal location of the gene

A 14.5 kDa barley endosperm protein that is a major allergen in bakers asthma disease, as previously shown by both in vitro (IgE binding) and in vivo tests, has been identified as a glycosylated monomeric member of the multigene family of inhibitors of α-amylase/trypsin from cereals. A cDNA encoding this allergen (renamed BMAI-1) has been isolated and characterized. The deduced sequence for the mature protein, which is 132 residues long, is identical in its N-terminal end to the 20 amino acid partial sequence previously determined from the purified allergen, and fully confirms that it is a member of the multigene family of cereal inhibitors. Southern-blot analysis of wheat/barley addition lines using the insert in the BMAI-1 cDNA clone as a probe, has led to the location of the allergen gene (Iam1) in barley chromosome 2, while another related member of this protein family, the barley dimeric α-amylase inhibitor BDAI-1 gene (Iad1) has been located in chromosome 6. Iam1 is the first member of this inhibitor family in cereals to be assigned to chromosome group 2, thus extending the dispersion of genes in the family to five out of the seven homology groups of chromosomes in wheat and barley (chromosome 2, 3, 4, 6 and 7).

A tetrameric inhibitor of insect α-amylase from barley

A tetrameric inhibitor that is active against α‐amylase from the larvae of the insect Tenebrio molitor, but inactive against the enzyme from human saliva and against the endogenous one, has been described in barley endosperm. The subunits of the inhibitor have been identified as the previously characterized proteins CMa, CMb and CMd, of which only CMa was inhibitory by itself.

The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.).

SummaryA cDNA encoding trypsin inhibitor CMe from barley endosperm has been cloned and characterized. The longest open reading frame of the cloned cDNA codes for a typical signal peptide of 24 residues followed by a sequence which is identical to the known amino acid sequence of the inhibitor, except for an Ile/Leu substitution at position 59. Southern blot analysis of wheat-barley addition lines has shown that chromosome 3H of barley carries the gene for CMe. This protein is present at less than 2%–3% of the wild-type amount in the mature endosperm of the mutant Risø 1508 with respect to Bomi barley, from which it has been derived, and the corresponding steady state levels of the CMe mRNA are about I%. One or two copies of the CMe gene (synonym Itc1) per haploid genome have been estimated both in the wild type and in the mutant, and DNA restriction patterns are identical in both stocks, so neither a change in copy number nor a major rearrangement of the structural gene account for the markedly decreased expression. The mutation at the lys 3a locus in Risø 1508 has been previously mapped in chromosome 7 (synonym 5H). A single dose of the wild-type allele at this locus (Lys 3a) restores the expression of gene CMe (allele CMe-1) in chromosome 3H to normal levels.