Luís C. Silva

Microextraction and gas chromatography/mass spectrometry for improved analysis of geosmin and other fungal "off" volatiles in grape juice

Geosmin is a volatile fungal metabolite with an earthy aroma produced in grape products from rotten grapes. The accumulation of geosmin in grapes is caused by the interaction of Botrytis cinerea and Penicillium expansum. Solid Phase Microextraction (SPME) has great utility for collecting volatile compounds in wine. However, contamination with earthy odours may have occurred previously in the must and novel methods are required for this commodity. In the present report, several parameters of the SPME were evaluated to optimize geosmin extraction. The method permitted quantification of geosmin and other fungal volatiles by Gas Chromatography-Mass Spectrometer (GC-MS) at very low concentrations. Limits of detection and quantification (L(D) and L(Q)) for geosmin were 4.7 ng L(-1) and 15.6 ng L(-1) respectively. The RSD was 4.1% and the recovery rates ranged from 115% to 134%. Uniquely, haloanisoles were analyzed by using only one internal standard (2,3,6-trichloroanisole) thus avoiding the synthesis of deuterated anisole analogues that are used as internal standard in other methods. The method was used for the analysis of grape juice samples inoculated with B. cinerea and P. expansum. Geosmin and methylisoborneol were the compounds that appeared to contribute most to earthy odours, although other fungal compounds which are claimed to cause earthy or mouldy off-odours were detected (e.g. 1-octen-3-ol and fenchol).

Nonlinear Discrete Homogenized Model for Out-of-Plane Loaded Masonry Walls

AbstractThis paper presents a simple and reliable homogenization approach coupled with rigid elements and homogenized interfaces for the analysis of out-of-plane loaded masonry panels. The homogeni...

Dynamic Behaviour Analysis of an English-Bond Masonry Prototype Using a Homogenized-Based Discrete FE Model

Full Finite Element strategies (the so called micro- and macro- models) are still nowadays the most used ones for the study of large masonry structures. However, macro-modelling still lacks accuracy at a meso-scale in terms of damage localization. On the other hand, micro-models are rather computational demanding and require a cumbersome modelling stage. Thus, homogenization-based frameworks give considerable advantages. Moreover, the study of English bond masonry appears to be disregarded in comparison to the running bond one. On this behalf, a two-step procedure based on homogenization theory is herein presented for the dynamic study of English-bond masonry structures. The presented homogenization approach uses two models at a micro-scale: (i) a plane-stress FE discretization within the concepts of Kirchhoff-Love plate theory; and (ii) a three-dimensional micro-model accounting with the mortar joint discontinuity existent at the thickness direction. Bricks are meshed with elastic elements with linear interpolation and joints are reduced to interfaces which obey to the nonlinear behaviour described by the so-called combined cracking-shearing-crushing model. The procedure allows obtaining homogenized bending moment/torque curvature relationships to be used at a structural level within a FE discrete model implemented in a commercial code. The model relies in rigid quadrilateral elements interconnected by homogenized bending/torque nonlinear springs. The framework is used to study the dynamic behaviour of an English-bond masonry wall benchmark. A macroscopic strategy is also considered to enrich the study. The numerical results are compared with the experimental data and a good agreement has been found.

Derivation of the out-of-plane behaviour of an english bond masonry wall through homogenization strategies

This work was supported by FCT (Portuguese Foundation for Science and Technology), within ISISE, scholarship SFRH/BD/95086/2013. This work was also partly financed by FEDER funds through the Competitivity Factors Operational Programme - COMPETE and by national funds through FCT - Foundation for Science and Technology within the scope of the project POCI-01-0145-FEDER-007633.

A discrete macro-model using homogenization with strain-rate dependency for the out-of-plane study of masonry panels subjected to impact loading

This work was supported by FCT (Portuguese Foundation for Science and Technology), within ISISE, scholarship SFRH/BD/95086/2013. This work was also partly financed by FEDER funds through the Competitivity Factors Operational Programme - COMPETE and by national funds through FCT – Foundation for Science and Technology within the scope of the project POCI-01-0145-FEDER-007633.