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Dive into the research topics where Luis Maranga is active.

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Featured researches published by Luis Maranga.


Vaccine | 2011

Bioprocess optimization for cell culture based influenza vaccine production

Kunal Aggarwal; Frank Jing; Luis Maranga; Jonathan Liu

Uncertainties and shortcomings associated with the current influenza vaccine production processes demand attention and exploration of new vaccine manufacture technologies. Based on a newly developed mammalian cell culture-based production process we investigated selected process parameters and describe three factors that are shown to impact productivity, process robustness and development time. They are time of infection, harvest time and virus input, or multiplicity of infection (MOI). By defining the time of infection as 4-5 days post cell seeding and harvest time as 2-3 days post-infection and comparing their effect on virus production, MOI is subsequently identified as the most impactful process parameter for live attenuated influenza vaccine (LAIV) manufacture. Infection at very low MOI (between 10(-4) and 10(-6) FFU/cell) resulted in high titer virus production (up to 30-fold productivity improvement) compared to higher MOI infections (10(-3) to 10(-2) FFU/cell). Application of these findings has allowed us to develop a platform process that can reduce the development time to approximately three weeks for an influenza vaccine manufacture process for new strains.


Biotechnology and Bioengineering | 2010

Production of cell culture (MDCK) derived live attenuated influenza vaccine (LAIV) in a fully disposable platform process.

Meena George; Masiha Farooq; Thi Dang; Bernadette Cortes; Jonathan Liu; Luis Maranga


Archive | 2009

Methods for cultivating cells, propagating and purifying viruses

Jonathan Liu; Mark Thompson; Luis Maranga; Floro Cataniag; Simon Hsu


Archive | 2007

Mdck cell lines supporting viral growth to high titers and bioreactor process using the same

Jonathan Liu; Richard Schwartz; Mark Thompson; Luis Maranga; Simon Hsu; Mridul Ghosh; Ajit Subramanian


Archive | 2010

Method of purifying influenza virus and removing MDCK cell DNA contaminants

Jonathan Liu; Richard B. Schwartz; Mark Thompson; Luis Maranga; Simon Hsu; Mridul Ghosh; Ajit Subramanian


Archive | 2007

Method for eliminating DNA contaminants from viral preparations

Jonathan Liu; Richard Schwartz; Mark Thompson; Luis Maranga; Simon Hsu; Mridul Ghosh; Ajit Subramanian


Archive | 2015

METHODS FOR CELL CULTURE, PROLIFERATION AND PURIFICATION OF VIRUS

Liu Jonathan; Thompson Mark; Luis Maranga; Floro Cataniag; Hsu Simon S


Archive | 2009

Verfahren zur zellkultivierung sowie vermehrung und reinigung von viren

Jonathan Liu; Mark Thompson; Luis Maranga; Floro Cataniag; Simon Hsu


Archive | 2008

Challenges and Solutions for the Next Generation of Vaccines: Development of Cell Culture-based Live Attenuated Influenza Vaccine

Jonathan Liu; Luis Maranga; Sachin Mani; Richard B. Schwartz


Archive | 2007

Líneas de células MDCK que soportan el crecimiento viral hasta altos títulos y proceso de biorreactor que las usa.

Jonathan Liu; Richard B. Schwartz; Mark Thompson; Luis Maranga; Simon Hsu; Mridul Ghosh; Ajit Subramanian

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