Luisa Machado Barin

Role of the adjunctive antimicrobial photodynamic therapy to periodontal treatment at plasmatic oxidative stress and vascular behavior

BACKGROUND To evaluate for the first time in vivo the effects of methylene blue (MB) photosensitizer dissolved in ethanol in antimicrobial photodynamic therapy (aPDT) as adjuvant periodontal treatment, at plasmatic oxidative stress and vascular behavior in rat model. METHODS Wistar rats were divided into negative control (NC, no periodontitis) and positive control (PC, with periodontitis, without any treatment). The other groups had periodontitis and were treated with scaling and root planing (SRP); SRP+aPDT+MB dissolved in water (aPDT I); SRP+aPDT+MB dissolved in ethanol (aPDT II). The periodontitis was induced by ligature at the mandibular right first molar. At 7/15/30days, rats were euthanized, the plasma was used to determine oxidative stress parameters and gingival tissue for histomorphometric analysis. RESULTS PC showed higher thiobarbituric acid reactive substances levels in 7/15/30days. aPDT II was able to block the lipid peroxidation, especially between 15th and 30th days. Glutathione reduced levels were consumed in PC, aPDT I and II groups throughout the experiment. aPDT II increased the vitamin C levels which were restored in this group in the 30th day. aPDT II group showed the highest number of blood vessels. CONCLUSION In summary, the aPDT with MB dissolved in ethanol provides better therapeutic responses in periodontitis treatment.

Oral and oropharyngeal cancer: epidemiology and survival analysis

RESUMO Objetivo Avaliar o perfil epidemiologico e a taxa de sobrevida do câncer de boca e orofaringe de pacientes atendidos em um hospital universitario. Metodos Foi realizado um estudo transversal por meio dos laudos anatomopatologicos dos pacientes com câncer de boca e orofaringe atendidos em um hospital universitario, na Regiao Sul, entre janeiro de 2004 a dezembro de 2014. A partir destes laudos, foram coletadas informacoes sobre o paciente e o tumor. A taxa de mortalidade foi obtida do registro [...]

Antimicrobial photodynamic therapy with photosensitizer in ethanol improves oxidative status and gingival collagen in a short-term in periodontitis

BACKGROUND This study evaluated the antimicrobial photodynamic therapy (aPDT) effects using the methylene blue (MB) in ethanol 20% on systemic oxidative status and collagen content from gingiva of rats with periodontitis. METHODS Rats were divided into five experimental groups: NC (negative control; no periodontitis); PC (positive control; periodontitis without any treatment); SRP (periodontitis and scaling and root planing), aPDT I (periodontitis and SRP+aPDT+MB solubilized in water), and aPDT II (periodontitis and SRP+aPDT+MB solubilized in ethanol 20%). After 7days of removal of the ligature, the periodontal treatments were performed. At 7/15/30days, gingival tissue was removed for morphometric analysis. The erythrocytes were used to evaluate systemic oxidative status. RESULTS PC group showed higher lipoperoxidation levels at 7/15/30days. aPDT indicated a protective influence in erythrocytes at 15days observed by the elevation in levels of systemic antioxidant defense. aPDT II group was the only one that restored the total collagen area in 15days, and recovered the type I collagen area at the same time point. CONCLUSIONS aPDT as an adjunct to the SRP can induce the systemic protective response against oxidative stress periodontitis-induced and recover the gingival collagen, thus promoting the healing periodontal, particularly when the MB is dissolved in ethanol 20%.

Osteonecrose dos maxilares associada ao uso de bifosfonatos: uma revisão de literatura

Bisphosphonates (BPS) are antiresorptive agents routinely used in the management of bone diseases and metastatic malignancies neoplasm. Its mechanism of action inhibits the osteoclastic and angiogenic activity of bone tissue. However, its chronic use can raise Bisphosphonate-Related Osteonecrosis of the Jaws (BRONJ), mainly in patients submitted to previous trauma. This study aims to review therapeutic approaches of these patients, focusing on prevention and treatment of these pathologies. The literature has presented various proposals to treat the BRONJ, but sometimes it’s controversial. The treatment of these pathologies has been challenging, once the protocols are not always effective to control this condition. Thus, previously of BFS therapeutic, the health professional should realize all the needed procedures to adequate the oral environment of these patients, as well as to maintain a rigorous attendance of your oral condition. It’s essential the multidisciplinary approach between physician and dental surgeon to manage these patients. Descriptors: Odontology. Diphosphonates. Mandible. Maxilla. Therapeutics. *** Doutoranda do Curso de Pós-graduação de Ciências Odontológicas com ênfase em Patologia Bucal pela UFSM, RS, Brasil. (luisambarin@hotmail.com) *** Mestre em Ciências Odontológicas com ênfase em Patologia Bucal pela UFSM, RS, Brasil. (fernandampillusky@hotmail.com, marcelam4p@gmail.com) *** Professora Doutora Adjunta do Departamento de Patologia da UFSM, RS, Brasil. (cristianedanesi@gmail.com)