Lynda Little-Ihrig
University of Pittsburgh
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Publication
Featured researches published by Lynda Little-Ihrig.
Scientific Reports | 2016
Pawan Puri; Lynda Little-Ihrig; Uma Chandran; Nathan C. Law; Mary Hunzicker-Dunn; Anthony J. Zeleznik
Activation of protein kinase A (PKA) by follicle stimulating hormone (FSH) transduces the signal that drives differentiation of ovarian granulosa cells (GCs). An unresolved question is whether PKA is sufficient to initiate the complex program of GC responses to FSH. We compared signaling pathways and gene expression profiles of GCs stimulated with FSH or expressing PKA-CQR, a constitutively active mutant of PKA. Both FSH and PKA-CQR stimulated the phosphorylation of proteins known to be involved in GC differentiation including CREB, ß-catenin, AKT, p42/44 MAPK, GAB2, GSK-3ß, FOXO1, and YAP. In contrast, FSH stimulated the phosphorylation of p38 MAP kinase but PKA-CQR did not. Microarray analysis revealed that 85% of transcripts that were up-regulated by FSH were increased to a comparable extent by PKA-CQR and of the transcripts that were down-regulated by FSH, 76% were also down-regulated by PKA-CQR. Transcripts regulated similarly by FSH and PKA-CQR are involved in steroidogenesis and differentiation, while transcripts more robustly up-regulated by PKA-CQR are involved in ovulation. Thus, PKA, under the conditions of our experimental approach appears to function as a master upstream kinase that is sufficient to initiate the complex pattern of intracellular signaling pathway and gene expression profiles that accompany GC differentiation.
Endocrinology | 1992
N. Ravindranath; Lynda Little-Ihrig; Heidi S. Phillips; Napoleone Ferrara; Anthony J. Zeleznik
Endocrinology | 2003
Anthony J. Zeleznik; Deeksha Saxena; Lynda Little-Ihrig
Endocrinology | 2007
Deeksha Saxena; Rosalba Escamilla-Hernandez; Lynda Little-Ihrig; Anthony J. Zeleznik
Endocrinology | 2004
Deeksha Saxena; Rachid Safi; Lynda Little-Ihrig; Anthony J. Zeleznik
Endocrinology | 1992
N. Ravindranath; Lynda Little-Ihrig; Deborah Fairchild Benyo; Anthony J. Zeleznik
The Journal of Clinical Endocrinology and Metabolism | 2004
Anthony J. Zeleznik; Lynda Little-Ihrig; Suresh Ramasawamy
Endocrinology | 1993
Deborah Fairchild Benyo; Lynda Little-Ihrig; Anthony J. Zeleznik
The Journal of Clinical Endocrinology and Metabolism | 1991
Steven G. Bassett; Lynda Little-Ihrig; J. Ian Mason; Anthony J. Zeleznik
Endocrinology | 1995
Jeremy P. Somers; Deborah Fairchild Benyo; Lynda Little-Ihrig; Anthony J. Zeleznik