Lyubov I. Kolesnikova

P37 State of health of teenagers – tofalars, living in the north of the irkutsk region

Background and aims Tofalars are the inhabitants of the Irkutsk region in Russia, living in highland zone. They concern to the small population groups of the North (756 persons). Last year’s replacement of traditional type of food (many proteins of animal origin) by the European one is marked. Tofalars concern to the Mongoloids. The aim is to estimate state of health and indicators of blood serum at teenagers – tofalars and Caucasian. Methods Fifty-four teenagers aged from twelve to eighteen years old were examined in Alygdzher settlement. Thirty-two of which are tofalars (seventeen boys and fifteen girls) and twenty-two Caucasian (twelve and ten girls). There were no teenagers with overweight among examined ones; the smaller linear dimension is typical to tofalars in comparison with Caucasians living on this territory. Spectrophotometric and fluorometric methods for studying the lipid peroxidation and antioxidant status have been used. Results Endocrine system diseases and eating disorders, musculoskeletal system diseases were more often marked at tofalars in comparison with Caucasians (p<0.05). It was established that the average level of total cholesterol and cholesterol of low-density lipoproteins at tofalars more low than at Caucasians (p<0.05). The low levels of lipid peroxidation products – ketodiens and conjugated triens are marked at tofalars, it’s 1,36 times lower (p<0.001) in comparison with Caucasians and also decrease of antioxidants indicators – superoxide dismutase and retinol (1,26 times lower, p<0.05). Conclusions The differences in health status between tofalars and Caucasians have been observed. The probable explanation to this serves as genetic factors and metabolism features at tofalars in comparison with Caucasians living at the same conditions.

P147 Lipid peroxidation processes in newborns born from mothers who consumed alcohol during pregnancy

Background and aims The period of organogenesis in the embryonic development is critical in terms of occurrence of long-term effects of intoxication. There is evidence that the fetus is exposed to the same alcohol level as the mother’s body. It is known that an imbalance in the system ‘lipid peroxidation-antioxidant defense’ leads to the development of oxidative stress, which is accompanied by a decrease in the body’s resistance to adverse factors of the external and internal environment. The aim of this study was to identify features of the lipid peroxidation-antioxidant defense system (LPO-AOD) in newborns born from mothers who consumed alcohol during pregnancy. Methods An umbilical cord blood of 66 newborns in the control group (group 1) and 74 newborns, whose mothers consumed alcohol in beer form, dry wine and champagne for the entire period of pregnancy (53 newborns, whose mothers consumed less than 2 doses or 750 ml (group 2) and 21 newborns whose mothers consumed from 2 to 11 doses (750–3850 ml)) (group 3) was studied. Spectrophotometric and fluorometric methods for studying the LPO-AOD have been used. All pregnant women received informed consent for the study. Results Lipid peroxidation process in both groups of newborns was characterised by a statistically significant increasing of diene conjugates content (p1−2=0,006; p1−3<0,001) and decreased values of α-tocopherol (p1−2=0,006; p1−3=0,031) and superoxide dismutase activity (p1−2<0,0001; p1−3=0,008) comparing to control group. The reduction of reduced glutathione (GSH) noted in group 2 in comparison with control (p1−2=0,0007). Fall GSH concentration in red blood cells in newborns 2nd and 3rd groups, may be associated with the observed significant decrease of glutathione reductase activity (p1−2<0,0001; p1−3<0,0001) and glutathione-S-transferase (p1−2=0,001; p1−3=0,04) comparing to control group. In general, the decrease in GSH concentration and activity of the enzymes involved in its metabolism in the newborn group 2 has a negative effect on the balance in the LPO-AOD system. Conclusions Thus, the results suggest that even a small amount of alcoholic beverages drunk by women during pregnancy can cause different metabolic changes in the newborns body, in particular, leading to an imbalance of redox exchange, expressed dysfunction of LPO-AOD system.