M. A. Benvenutti

The use of image analysis to determine the number and position of cattle at a water point

This work assessed the accuracy of animal counts and positions using image analysis.Counting accuracy decrease with distance to camera.Positional accuracy of independent points was 0.8?0.5m.Positional accuracy did not change with distance to the camera. This study assessed the application of an image analysis method to accurately determine the number and position of cattle which are critical inputs for enteric methane emission calculations using micrometeorological methods. Animal imagery was collected with three synchronised time-lapse cameras located at 7, 35 and 77m from a 20×30m water point enclosure containing 20 steers, recorded over three consecutive days. Four independent observers counted the number of animals visible in each of 516 images. The counting error increased with distance from the enclosure (0.1%, 3.7% and 15.4% of total animals) as a result of increased overlapping and decreased clarity of the animals on the image. Animal positions were estimated using a polynomial transformation of image coordinates (pixels) to map coordinates. The average location error (distance between estimated and actual position) of independent targets was 0.8?0.5m and did not change with distance to the camera. We conclude that the analysis of 12MP images from time lapse cameras can provide reliable and accurate estimates of the position and the number of animals located within 55m from the camera.

New candidate markers of phosphorus status in beef breeder cows

Determining the phosphorus (P) status of cattle grazing P-deficient rangelands in northern Australia is important for improving animal production in these areas. Plasma inorganic P concentration is currently the best diagnostic marker of dietary P deficiency in growing cattle but is not suitable for assessing the P status of breeder cows, which often mobilise substantial bone and soft tissue reserves in late pregnancy and lactation. Markers of bone turnover offer potential as markers of P status in cattle, as they reflect bone mobilisation or bone formation. Recent experiments investigating the physiology of beef breeder cows during diet P deficiency have indicated that the ratio of plasma total calcium concentration to plasma inorganic P concentration might be suitable as a simple index of P deficiency. However, a more specific measure of increased bone mobilisation in P-deficient breeders is plasma concentration of C-terminal telopeptide of Type 1 collagen. Also, plasma concentration of bone alkaline phosphatase is a marker of defective bone mineralisation in dietary P deficiency. These candidate markers warrant further investigation to determine their predictive value for P deficiency in cattle.

Non-interference measurement of CH4, N2O and NH3 emissions from cattle

A technique combining open-path Fourier transform infrared spectroscopy with an inverse-dispersion model was used to quantify methane (CH4), nitrous oxide (N2O) and ammonia (NH3) emissions from an isolated cattle pen in south-eastern Australia. Twenty-eight Angus steers (1-year old, initial average liveweight 404 kg) were fed a 60% grain diet and kept in a pen (20 × 20 m) for 41 days. Gas concentrations were measured downwind of the pen using an open-path Fourier transform infrared spectroscopy with a path length of 100 m, having a detection sensitivity of 2, 0.3 and 0.4 ppb for CH4, N2O and NH3, respectively. Daily emission rates were 232, 14 and 192 g/cattle.day for CH4, N2O and NH3, respectively. The measured CH4 emissions were in agreement with predictions based on Australian National Inventory recommendations, however, measured N2O and NH3 emissions were much higher than the predicted values. Extrapolation of our measurements would mean that CH4 and N2O emissions from beef feedlot cattle contribute 3.1% and 5.9% of the Australian agricultural CH4 and N2O emissions, respectively.

Utilising mobilisation of body reserves to improve the management of phosphorus nutrition of breeder cows

Phosphorus (P) deficiency is a major constraint to the productivity of breeder herds grazing low-P rangelands due to adverse effects on growth and fertility. However, P supplementation during the wet season, when additional dietary P is most needed, is often difficult due to practical constraints. Body P reserves in breeders can be mobilised and alleviate dietary P deficiency within an annual cycle. Approaches to estimate bone P reserves and net mobilisation or replenishment of P from the analysis of rib and hip (tuber coxae) biopsies are discussed. In at least some circumstances, breeder cows grazing P-deficient pastures mobilise bone P to alleviate the effects of diet P deficiency. Recent experiments with breeders have investigated mobilisation of body P to alleviate the adverse effects of dietary P deficiency during pregnancy and early lactation, and subsequent replenishment of body P reserves. Both mature cows and first-calf cows (FCC) calving in a high P status and fed severely P-deficient diets during lactation were able to mobilise sufficient body P reserves to provide milk for moderate calf growth (viz. 0.6-0.8 kg liveweight (LW)/day for 3 months), but this was associated with rapid cow-LW loss and markedly decreased bone P content. First-calf cows appear to have lesser capacity than mature cows to mobilise body P. FCC fed P-adequate diets during late pregnancy maintained high voluntary intakes and had higher LW and bone P reserves at calving. When fed a P-deficient diet during early lactation, these greater bone P reserves were utilised. Bone P reserves can be replenished by P-adequate diets fed post-weaning. Breeder management that relies more on mobilising body P reserves when P demands are high and on replacing these body P reserves when P demands are lower offers an opportunity to alleviate the effects of dietary P deficiencies during the early wet season in situations where P supplementation is not possible.

Applicability of Eddy Covariance to Estimate Methane Emissions from Grazing Cattle

Grazing systems represent a significant source of enteric methane (CH), but available techniques for quantifying herd scale emissions are limited. This study explores the capability of an eddy covariance (EC) measurement system for long-term monitoring of CH emissions from grazing cattle. Measurements were made in two pasture settings: in the center of a large grazing paddock, and near a watering point where animals congregated during the day. Cattle positions were monitored through time-lapse images, and this information was used with a Lagrangian stochastic dispersion model to interpret EC fluxes and derive per-animal CH emission rates. Initial grazing paddock measurements were challenged by the rapid movement of cattle across the measurement footprint, but a feed supplement placed upwind of the measurements helped retain animals within the footprint, allowing emission estimates for 20% of the recorded daytime fluxes. At the water point, >50% of the flux measurement periods included cattle emissions. Overall, cattle emissions for the paddock site were higher (253 g CH m adult equivalent [AE] d, SD = 75) and more variable than emissions at the water point (158 g CH AE d, SD = 34). Combining results from both sites gave a CH production of 0.43 g kg body weight, which is in range of other reported emissions from grazing animals. With an understanding of animal behavior to allow the most effective use of tower placement, the combination of an EC measurement platform and a Lagrangian stochastic model could have practical applications for long-term monitoring of fluxes in grazing environments.

Long-term assessment of a new rotational-grazing management strategy called PUP-grazing (proportion of un-grazed pasture)

A new rotational-grazing management strategy called PUP-grazing (proportion of un-grazed pasture, which is the estimated percentage of pasture vegetation without signs of being consumed) was compared with the traditional rotational-grazing management strategy (TGMS, which is based on residual sward height) using Brahman cross steers on Axonopus catarinensis over two periods (2013/14 and 2015/16) in Misiones, Argentina. The proportion of un-grazed pasture for PUP and TGMS was 11.5 and 3.4%, respectively, of the pasture area. Average daily liveweight gain/animal was significantly higher for PUP than for TGMS (606 vs. 420 g/d; P<0.05) while liveweight gain per hectare was 35% greater for PUP (194 vs. 144 kg/ha/period). The gross margin per hectare was much higher for PUP than for TGMS (US

Ingestive behaviour and forage intake responses of young and mature steers to the vertical differentiation of sugarcane in pen and grazing studies

85.7 vs. 8.4/ha/period). These results indicate that on A. catarinensis pastures PUP-grazing has the potential for greater animal and economic performance than the TGMS. Keywords : Cattle, forage consumption, grazing management, gross margin, liveweight gain. DOI: 10.17138/TGFT(6)53-57

Effects of deferred forage as winter cover on spring growth of the tropical grasses Chloris gayana and Panicum coloratum

Sugarcane is an important forage resource in sub-tropical and tropical areas as it is used during the winter or dry season when the growth rate of pastures is significantly reduced. The current research study assessed the effect of four vertical sections of sugarcane in a pen trial and the level of sugarcane utilization in a grazing trial on the ingestive behaviour and forage intake of two age groups of steers (1 and 2 years old). The pen trial was comprised of two simultaneous 4 x 4 balanced Latin square designs (one for each age group of animals) of four periods, four animals and four feeding treatments, which consisted of four equal vertical sections of sugarcane. Dry matter (DM) and digestible DM (DDM) intake per kilogram of metabolic weight declined gradually from top to bottom of the sugarcane, with no significant differences between the age groups of steers. This difference in intake was associated with a decline in intake of neutral detergent fibre (NDF) as a proportion of the liveweight of the animal and an increase of total chewing time per kilogram of DM or NDF from top to bottom of the sugarcane. It was concluded that the toughness of plant material played a significant role regulating intake, which was higher for the top sections of sugarcane. In the grazing trial, steers of both age groups grazed down sugarcane in three plots over 9 days. Steers grazed up to four distinctive grazing strata. Digestible DM intake (DDM intake) was high at low levels of horizontal utilization of the top grazing stratum but DDM intake started to decline sharply when this stratum was removed in 0.92 of paddock area (i.e. equivalent to 0.08 of the pasture area remaining un-grazed). It was concluded that the proportion of un-grazed area of the pasture can be used as a grazing management strategy to control forage intake for sugarcane.

The effect of the density and physical properties of grass stems on the foraging behaviour and instantaneous intake rate by cattle grazing an artificial reproductive tropical sward

This pot study assessed the effects of deferring forage during autumn and leaving as winter cover on reducing cold damage to plants of 2 tropical (C4) grasses ( Chloris gayana and Panicum coloratum ) in a temperate environment in La Plata, Buenos Aires, Argentina. Mature plants were subjected to the following treatments: (i) autumn-deferred forage retained (DF) as control; and (ii) autumn-deferred forage removed (DFR) cutting at 15 cm from soil level at beginning of winter. This experiment had 10 replicates per treatment and 1 plant per pot (experimental unit). Plants of both species were extracted from a commercial beef farm and transplanted into an experimental garden in pots where they grew outdoors from 2 February to 23 May (111 days) when treatments were applied. After winter, both grasses were cut to 15 cm in early spring (27 September) and spring growth was measured in November. Shoot biomass was harvested at ground level and separated into lower and upper layers (above and below 15 cm), leaf blades, sheaths and stolons. Daily air temperature, relative humidity and frost events were registered. Allometric analysis of shoot biomass was performed to determine the stress incidence by cold. DF plants achieved 55‒80% higher shoot biomass than DFR plants during spring in both species. The allometric analysis revealed for P. coloratum significant relationships between shoot biomass from plant compartments (lower and upper layers, leaf blade and sheath) and total shoot biomass in both treatments, indicating good cold tolerance. However, for C. gayana, unlike DF plants, DFR plants were strongly stressed, showing a lack of shoot biomass fit. These results suggest that deferring autumn forage growth and retaining as winter cover may improve survival during winter and productivity during spring of these two tropical grasses in temperate pastoral systems. However, the study needs to be repeated under field conditions and under grazing or cutting over a number of years in different situations to verify these preliminary results. Keywords : Winter cover, cold damage, frost protection, pasture management.