M. A. Díez-Rojo

New host races of Meloidogyne arenaria, M. incognita, and M. javanica from horticultural regions of Spain.

In total, 140 populations of the genus Meloidogyne collected from representative horticultural regions of Spain and maintained at the Department of Agroecology, Institute of Agrarian Sciences, Madrid were characterized using perineal morphology, sequenced characterized amplified region polymerase chain reaction with species-specific primers, and bioassay. Using a modified North Carolina differential host test as a basis, 13 Meloidogyne populations did not fit into the published race scheme. These populations had very limited host ranges, reproducing only on susceptible tomato cv. Marmande and sometimes also on tobacco cv. NC95 and the pepper cv. Sonar in the case of Meloidogyne arenaria. They did not reproduce on cotton or peanut. The species and new races were identified as M. incognita races 5 (6 populations) and 6 (2 populations) and M. javanica race 5 (2 populations), with new records of M. arenaria race 3 (2 populations) and M. javanica race 1 (23 populations) for Spain.

Potential Insect Vectors of Bursaphelenchus spp. (Nematoda: Parasitaphelenchidae) in Spanish Pine Forests

Potential insect vectors of Bursaphelenchus xylophilus (PWN) were studied. Pathways of introduction of PWN from Portugal to Europe, through Spain, were determined and traps were located in pine stands sites along the pathways. 19 Cerambycidae, 12 Scolytidae, 12 Buprestidae and 10 Curculionidae species have been found. Trapped insects were examined for the presence of nematodes under their elytra. Nematodes were found on Arhopalus ferus, Spondylis buprestoides, Hylastes ater, Hylurgus lingniperda, Orthotomicus erosus, Pityogenes bidentatus, Tomicus piniperda, Hylobius abietis and Pissodes validirrostris specimens. Monochamus galloprovincialis was the most important insect species, representing a risk for the introduction of the PWN in Spanish pine forests; Cerambycidae and Curculionidae species, were taken into account because they have been reported as vectors of other Bursaphelenchus spp.