M. Brambilla

Evaluation of simplified covering systems to reduce gaseous emissions from livestock manure storage

Ammonia, methane, and carbon dioxide are the primary atmospheric emissions from cattle and pig farms. A significant part of these emissions is produced by the decomposition of slurry organic matter during manure storage and treatment phases. Present solutions to contain emissions from storage lagoons generally involve reducing the free surface of the slurry by covering it either with permanent fixed structures or temporary floating ones. This study investigated the effectiveness of five simple floating covers in reducing emissions from pig and cattle slurry. The coverings included vegetable oil (a mixture of rapeseed and soybean oil), expanded clay, chopped maize stalks, chopped wheat straw, and chopped wood chips. All were tested at two different thicknesses: 70 and 140 mm for solid coverings, and 3 and 9 mm for liquid. Slurry samples covered with the above-mentioned materials were placed in nine stainless steel airtight cylinders measuring 190 dm3. Gaseous and odor concentrations in the headspace were monitored using a Bruel & Kjaer 1302 multi-gas monitor and a T07 olfactometer. The flotation aptitude of the different coverings was also tested. Results revealed substantial differences in ammonia emission reduction efficiency (1% to 100%) and odor abatement (0% to 90%), and high levels of reduction efficiency were achieved by all the tested covers at the higher thickness. However, equally valid results were not obtained for methane emissions reduction. In regard to flotation aptitude and cover deterioration on slurry, expanded clay and wood chips demonstrated long-term resistance to both deterioration and sinking.

Foliar and root uptake of 134Cs, 85Sr and 65Zn in processing tomato plants (Lycopersicon esculentum Mill.).

The results of an experimental study on the behaviour of 134Cs, 85Sr and 65Zn in processing tomato plants grown in peat substrate are presented. Plants were contaminated by wet deposition of 134Cs, 85Sr and 65Zn, either by sprinkling the above ground part at two phenological stages or by administering 134Cs, 85Sr and 65Zn to the soil. The plants contaminated at the second phenological stage intercepted 38.3% less than those contaminated at the first stage, although leaf area increased by more than double. Transfer coefficients from peat soil to ripe fruit for 134Cs are significantly higher than those for 85Sr and 65Zn. Leaf to fruit transfer coefficients for 134Cs are one order of magnitude higher than for 65Zn and two orders higher than for 85Sr. Only when deposition affects fruits, as at the second phenological stage, are transfer coefficients to fruits similar for the three radionuclides.

Pelletization of composted swine manure solid fraction with different organic co-formulates: effect of pellet physical properties on rotating spreader distribution patterns

IntroductionIn Europe, because of the high production levels of livestock farming in general and pig farming in particular, animal waste management has become increasingly important to comply with the required lowering of livestock farming environmental pressure. Usually manures undergo solid/liquid separation, which generates one clarified liquid fraction and one nutrient-rich solid fraction suitable for in farm composting (both raw and in mixture with other bulking agents). This can be used to produce soil amendments, whose management can be further improved by pelletization that, against technological and environmental advantages, has the disadvantage of requiring a quite high energy input.ResultsFour different pelleted organic fertilizer formulations made of swine manure solid fraction (SMSF) composted both by itself and with sawdust (SMSF-SD), wood chips (SMSF-WC) and wheat straw were tested to highlight differences in physico-chemical and land distribution features. They were compared with two pelleted organic fertilizers ordinarily available at retailers. Results show that, as far as physical and chemical features are concerned, the greatest difference from the reference products used in this study is found in pellet size distribution after spreading since the disintegrating action of the rotating vanes does not affect the tested formulation with the same intensity as the commercial products. Distribution tests showed that SMSF-SD was the formulation with better longitudinal and transverse distribution, while SMSF was the one showing good transverse but poor longitudinal distribution.ConclusionsIn farm pelletizing of SMSF composted with different organic waste materials as co-formulates can turn into organic fertilizer formulations comparable with pelleted organic fertilizers ordinarily available at retailers. SMSF-WC was the formulation with the best resistance to fragmentation induced by spreader vanes. SMSF-SD was the formulation showing better longitudinal and transverse distribution, while SMSF showed good transverse but poor longitudinal distribution. These promising results enhance the importance of co-composting as a way to increase livestock farming sustainability and produce better manure compost for wider agricultural uses.

Hepatitis E Virus (Genotype 3) in Slurry Samples from Swine Farming Activities in Italy.

Hepatitis E virus (HEV) is an emergent causative agent of acute hepatitis, transmitted by fecal–oral route. Infection with HEV is a global cause for morbidity and mortality throughout the world: it mainly causes large outbreaks in endemic areas and sporadic autochthonous cases in industrialized countries where HEV infections seem to be an emergent zoonotic disease. Infection of porcine livestock and its relationship with the human cases have been demonstrated. The present study describes an investigation on the prevalence and diversity of HEV in pig slurry in Italy. Slurry samples (24) were collected from ten farms located in North Italy during 2015 and analyzed for HEV, using four broad-range nested PCR assays targeting ORF1 (MTase), ORF2 (capsid) genes, and ORF2/3 regions. Overall, 18 samples (75%) were positive for HEV RNA, and characterized as genotype 3. Nine samples could be subtyped by ORF2 sequencing: Eight belonged to subtype 3f, while one sequence could not be characterized by blast analysis and phylogenetic analysis and may actually represent a new subtype. Furthermore, similarity of 99% was found between 3f Italian HEV sequences of human and swine origins. Real-Time PCR assay was also performed, in order to obtain quantitative data on positive samples. Two swine slurry samples were positive, containing 600 and 1000 UI per mL of sewage. The results of this study show that HEV strains belonging to zoonotic genotype 3 are widely present in swine excreta, and have high degree of identity with strains detected in autochthonous HEV cases. Improving swine farming operations safety and increasing operators’ awareness of the zoonotic potential connected with the handling of swine effluents turn out to be key points in order to reduce the environmental and sanitary problem represented by the possible dissemination of HEV to water bodies.

Radionuclides Behavior in Fruit Plants

This paper summarizes research carried out on fruits by the Universita Cattolica del Sacro Cuore (UCSC) in Piacenza, Italy. Among the fruit crops studied, strawberry, blackberry, grapevine, apple, pear, and olive, research on strawberry and blackberry was funded by the Food Standard Agency (UK). Fruit plants were grown in pots, kept under tunnels or in open field, and contaminated with 134Cs and 85Sr via leaves or via soil. Interception in strawberry plants ranges 39–17 % for 134Cs, from anthesis (April) to predormancy (November). Leaf-to-fruit translocation occurs to a greater extent for 134Cs than for 85Sr. The distribution of contamination in fruit crops is an element-specific process: 134Cs is preferentially allocated to fruits and 85Sr to leaves. However, the activity in leaves is also species-specific: fruit species show different leaf-to-fruit translocation. Results on apple, pear, and grape crops indicate that the highest transfer from leaf to fruit occurs in apple crops. Olive plants also show 134Cs translocation from leaves to trunks. Grapevines grown on mineral soil show a root uptake higher for 85Sr than for 134Cs, while strawberries grown on a peat substrate show a root uptake higher for 134Cs than for 85Sr. Rinsing directly contaminated fruits removes 85Sr (36 %) to a greater degree than 134Cs (24 %). Transfer to olive oil is low. A 57 % of 134Cs is transferred from grapes to white wine.

Case Study of the Monitoring of Ultra-High Temperature Processed Partly Skimmed Milk Production Batches by Means of an Electronic Nose

Smell and flavor are key factors that determine the acceptance of food by consumers. With reference to milk production, some production batches, despite passing routine microbiological and physical-chemical standards, exhibit bad odors for many reasons related to the production process itself (from fresh milk delivery to bottling). In this article, we present a case study of the capability of an electronic nose in discriminating different qualities of milk by sensing the aroma of milk samples belonging to different production batches and to different brands. All the analyzed milk samples were ultra-high temperature (UHT) processed, partly skimmed, and commercially available at retailers. Milk samples showing off-flavors were compared with other samples of the same kind of milk belonging to the same brand (but to different production batches) and to different brands. The comparison was performed by comparing the smell of the samples just after opening the packaging and again 2 h later. In all cases, principal component analysis was able to discriminate samples into two different groups (normal and anomalous). Moreover, analysis of the olfactory fingerprints showed that 2 h after the opening of the packaging, the flavor of anomalous samples evolved in a different way from that of the normal samples. The results confirm the utility of the e-nose approach in monitoring the quality of UHT partly skimmed milk production batches, especially if combined with chemical, physical, and sensory techniques.