M. Francis

High-Performance Thin-Layer Chromatographic Determination of Rosiglitazone in Its Dosage Form

A high-performance thin layer chromatographic method has been developed for the determination of rosiglitazone in pharmaceutical preparations. This method uses silica gel 60F254 as the stationary phase, ethyl acetate-toluene-methanol, 45 + 55+1 (v/v) as mobile phase. Detection was performed at 242 nm and pioglitazone hydrochloride was used as internal standard. The response was found to be linearly dependent on amount of rosiglitazone between 100 and 1200 ng. The method was validated to determine its accuracy and precision. System-suitability tests were conducted to verify that the resolution and reproducibility of the system were adequate for the analysis. The determination of rosiglitazone in tablets is described.

Determination of Doxazosin in its Pharmaceutical Formulation by High-Performance Thin-Layer Chromatography

This paper describes a high-performance thin-layer chromatographic method developed for determination of doxazosin in its pharmaceutical formulation. The analysis was performed on aluminum foil-backed silica gel 60 F254 HPTLC plates with ethyl acetate-methanol, 9 + 1 (v/v) as mobile phase. Detection was performed at λ = 277 nm. Terazosin hydrochloride, a structurally similar molecule was used as internal standard. The developed method was subjected to statistical validation to determine its accuracy and precision. The repeatability was also determined. System suitability tests were performed to verify that the resolution and reproducibility of the chromatography was adequate for the analysis to be done. The determination of doxazosin in tablets is described.