M. J. Cañal
University of Oviedo
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Featured researches published by M. J. Cañal.
Journal of Plant Physiology | 2009
Mª. Estrella Santamaría; Rodrigo Hasbún; Ma José Valera; Mónica Meijón; Luis Valledor; J. Rodríguez; Peter E. Toorop; M. J. Cañal; Roberto Rodríguez
The relationships between genomic DNA cytosine methylation, histone H4 acetylation and bud dormancy in Castanea sativa are described. Acetylated H4 histone and genomic DNA methylation patterns showed opposite abundance patterns during bud set and bud burst. Increased and decreased methylation levels in the apical buds coincided with bud set and bud burst, respectively. Intermediate axillary buds were characterized by constant levels of DNA methylation during burst of apical buds and reduced fluctuation in DNA methylation throughout the year, which coincided with the absence of macro-morphological changes. Furthermore, acetylated histone H4 (AcH4) levels from apical buds were higher during bud burst than during bud set, as was demonstrated by immunodetection. Results were validated with three additional C. sativa provenances. Thus, global DNA methylation and AcH4 levels showed opposite patterns and coincided with changes in bud dormancy in C. sativa.
Sexual Plant Reproduction | 2010
Marcos Viejo; Roberto Rodríguez; Luis Valledor; Marta Pérez; M. J. Cañal; Rodrigo Hasbún
From anthesis to mature seed formation, burrs from cross-pollinated adult Castanea sativa Miller trees were characterized and seven developmental stages defined based on macro and micromorphological traits. In order to get an insight into the involvement of epigenetic mechanisms in sexual embryogenesis and to define somatic embryogenesis induction capability, global DNA methylation and the somatic embryogenic competence were quantified. On cross-pollinated trees once fertilization takes place, at least one ovule per ovary becomes dominant, and transient DNA demethylation occurs coinciding with the start of the sexual embryogenic programme. Unfertilized ovules from the same cluster, which maintain their prior size, increase their methylation level and undergo degeneration. These results were validated using non-cross-pollinated trees and the asynchrony of flower receptivity. When testing in vitro somatic embryogenesis response of isolated dominant ovules and axes from zygotic embryos under cross-pollinated conditions, the highest competence was found for reaching seed maturity. Thus, a “developmental window” of somatic embryogenesis in chestnut has been characterized. It includes from fertilization to embryo maturity, and a transient decrease in methylation is necessary after fertilization for the development of the somatic embryogenesis response.
Journal of Plant Physiology | 1999
P. Moncaleán; M. J. Cañal; Isabel Feito; Ana Rodríguez; Belén Fernández
Summary Benzyladenine (BA) uptake and metabolism, zeatin-type cytokinins and residual macronutrients in the culture medium were measured after 0, 0.5, 1, 2, 8 and 16 hours of culture in Actinidia deliciosa explants cultured in liquid medium using cellulose plugs as support of the explants. At the end of the culture period, low percentages of hyperhydric shoots were found. During the first 30 min of culture the amount of BA in the culture medium dramatically decreased. 8-[ 14 C] BA was rapidly metabolized by rootless shoots of kiwifruit cultured in liquid medium and it could be the reason of the low percentage of hyperhydric shoots. The levels of zeatin-type cytokinins remained constant during the first 16 h of culture, which could suggest that BA acts «per se» and not through endogenous cytokinins in the development of kiwifruit explants. Ammonium and phosphate were mainly uptaken during the first hours of culture and these ions were the most consumed at the end of the culture period (35 days). MS medium could be too rich for the culture of kiwifruit in liquid medium except for phosphate which concentration should be even increased.
Plant Growth Regulation | 1997
Ângelo Kidelman Dantas de Oliveira; M. J. Cañal; Ma Luz Centeno; Isabel Feito; Belén Fernández
Endogenous indole-3-acetic acid (IAA), abscisic acid (ABA) and cytokinins (zeatin, zeatin riboside, dihydrozeatin, (diH)Z, dihydrozeatin riboside, (diH)[9R]Z, N6-isopentenyl adenine and N6-isopentenil adenine riboside) levels were evaluated in normal (N) and hyperhydric (H) microplants of Dianthus caryophyllus cultured under different aeration conditions in hormone-free liquid medium. The morphological differences between N and H explants grown under ventilated conditions were correlated with differences in their endogenous hormonal levels: after 15 and 30 days of culture, H explants showed lower IAA and ABA contents than N explants, as well as higher cytokinin levels, mainly of (diH)Z and (diH)[9R]Z. This was associated with less tissue differentiation and with an inability of H microplants to survive under ex vitro conditions. However, these relationships could not be observed between H and N explants grown under non-ventilated conditions probably due to the difficulty in discerning the plant status (N or H) and therefore, an underestimation of H microplants. This assumption is supported by the low ability for acclimatization to ex vitro of N plants grown without ventilation.
Journal of Plant Biochemistry and Biotechnology | 2009
Luis Valledor; Rodrigo Hasbún; Roberto Rodríguez; M. J. Cañal
A simple method for extracting DNA from various in vitro or ex vitro Pinus tissues is described. Good yield and high quality RNA-free DNA ready to use in molecular biology assays or analytical analysis was obtained. DNA quality was measured analyzing absorption spectra between 200 and 300 nm, A260/280 ratio and HPCE. This protocol was tested with no modification in a wide range of Pinus tissues from recalcitrant in vitro callus to mature field scions improving the results obtained with previous protocols. As the protocol is simple, almost universal and inexpensive it may be used for routine isolation from Pinus tissues. An isolation troubleshooting table was included to facilitate the setting up of reported protocol to other plant species.
Plant Growth Regulation | 2000
M. J. Cañal; H. Fernández; P. Fernández; Ma Luz Centeno; Belén Fernández
Uptake and metabolism of 6-benzyladenine (BA) werestudied in Actinidia deliciosa explants grown onventilated liquid Murashige and Skoog medium, usingcellulose plugs as explant support, after 0.5, 1, 2,8, 16, and 24h, 15 and 35 days of exposure to 14.8 kBqof 8-[14C] BA. Absorption of the BA is a rapidprocess and in the first half hour of culture 65% ofthe initial amount had disappeared from the medium.Exogenous BA was transformed into 7-β-D-glucopyranosyl-BA, 9-β-D-glucopyranosyl-BA, [9G]BA, 3-β-D-glucopyranosyl-BA, 9-β-D-ribofuranosyl-BA, [9R]BA, adenine, adenosine,and 5′-monophosphate of [9R]BA, [9R-5′P]BA. During thefirst 8h BA levels in the culture medium decreased,being converted into the active forms [9R]BA and[9R-5′P]BA. The excess of BA absorbed was inactivatedby glucosidation yielding [9G]BA. Ventilated culturesfavoured metabolization of active forms into inactivecompounds and this could be related to the lack ofhyperhydric shoots.
Plant Cell Tissue and Organ Culture | 2006
Sophie Roels; Carlos Noceda; Maritza Escalona; Jorge Sandoval; M. J. Cañal; Roberto Rodríguez; Pierre Debergh
Scientia Horticulturae | 2009
Mónica Meijón; Roberto Rodríguez; M. J. Cañal; Isabel Feito
Plant Cell Tissue and Organ Culture | 2000
Mª Teresa Bascarán Fernández; M. Fernández; Ma Luz Centeno; M. J. Cañal; Roberto Rodríguez
In Vitro Cellular & Developmental Biology – Plant | 2010
Luis Arigita; M. J. Cañal; Ricardo Sánchez Tamés; A. González