M. Kaida
Osaka City University
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Publication
Featured researches published by M. Kaida.
Japanese Journal of Ophthalmology | 2003
Yoko Miura; Nobuyo Yanagihara; Hitoshi Imamura; M. Kaida; Mitsuyasu Moriwaki; Kunihiko Shiraki; Tokuhiko Miki
PURPOSE A defect in retinal pigment epithelial (RPE) cells may cause dysfunction of the neural retina, so rapid recovery of differentiated RPE cells is required after RPE injury. We investigated the effect of hepatocyte growth factor (HGF) on wound healing in RPE cells. METHODS Confluent monolayers of bovine RPE cells were denuded, and the cells were allowed to recover in the presence or absence of HGF. The effect of HGF on RPE cell proliferation was evaluated by a 3-(4;5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetraz olium assay. In a migration assay, mitomycin C was used to inhibit proliferation, and the number of migrated cells was counted. The signaling pathways involved were examined using inhibitors of mitogen-activated protein kinase (MAPK), phosphatidylinositol-3 (PI3) kinase and protein kinase C pathways. RESULTS At 80 ng/mL, HGF stimulated the wound closure of RPE monolayers and rendered the restituted cells more epithelioid in shape. HGF at 10 ng/mL stimulated RPE cell migration the most, whereas 80 ng/mL of HGF inhibited migration, but stimulated proliferation the most. In particular, PI3 kinase and MAPK inhibitor inhibited PRE cell migration and proliferation, respectively. CONCLUSIONS HGF stimulated wound closure in cultured RPE cells, and rendered restituted cells epithelioid in shape. HGF may become a therapeutic candidate for RPE wound healing.
Journal of Cutaneous Pathology | 2011
Nao Kusutani; Hisashi Tamiya; Daisuke Tsuruta; Nobuyuki Mizuno; Junko Sowa; M. Kaida; Masamitsu Ishii; Osamu Yamamoto; Hiromi Kobayashi
Fig. 1. A) Multiple erythematous papules extensively involve the face. B and C) Large pale-staining histiocytes show cytophagocytosis (emperipolesis) of neutrophils and lymphocytes. There is an accompanying infiltrate spanning the full thickness of the dermis (hematoxylin and eosin staining; original magnification B: ×100; C: ×400). D) Terminal deoxy-UTP nick-end labeling (TUNEL) staining of the section showed phagocytized cells exhibiting apoptosis (original magnification, ×400).
Investigative Ophthalmology & Visual Science | 2000
M. Kaida; Feng Cao; Christine M. B. Skumatz; Pamela E. Irving; Janice M. Burke
Graefes Archive for Clinical and Experimental Ophthalmology | 2001
Nobuyo Yanagihara; Yoko Miura; Mitsuyasu Moriwaki; Kunihiko Shiraki; H. Imamura; M. Kaida; Tokuhiko Miki
Osaka city medical journal | 2011
Hisashi Iwami; Takeya Kohno; Manabu Yamamoto; M. Kaida; Norito Miki; Shinsuke Ataka; Kunihiko Shiraki
Investigative Ophthalmology & Visual Science | 2013
Takeya Kohno; Manabu Yamamoto; tasuku yoneda; Yusaku Yoshida; Hisashi Iwami; M. Kaida; M. Hirabayashi; Kunihiko Shiraki
Investigative Ophthalmology & Visual Science | 2012
Takeya Kohno; Manabu Yamamoto; Hisashi Iwami; M. Kaida; Yuusaku Yoshida; tasuku yoneda; M. Hirabayashi; Kunihiko Shiraki
Investigative Ophthalmology & Visual Science | 2012
M. Kaida; Takeya Kohno; M. Hirabayashi; Kunihiko Shiraki
Investigative Ophthalmology & Visual Science | 2011
Takeya Kohno; Manabu Yamamoto; Hisashi Iwami; Shinsuke Ataka; M. Kaida; M. Hirabayashi; Kunihiko Shiraki
Investigative Ophthalmology & Visual Science | 2011
Megumi Yatera; Takeya Kohno; Manabu Yamamoto; Yusaku Yoshida; tasuku yoneda; Hisashi Iwami; M. Kaida; Yoshio Okazaki; Kunihiko Shiraki