M. Kreisler

DR7 and DQ2 are positively associated with immunoglobulin-E response to the main antigen of olive pollen (Ole e I) in allergic patients

We have studied the relationship between HLA class II haplotypes and alleles, and the IgE antibody response to a highly purified allergen, Ole e I, in allergic patients. Ole e I, is the major antigen from the pollen of olive tree that grows mainly in the Mediterranean. Genomic DNA typing was performed in 40 unrelated patients with seasonal allergic pollenosis who had specific IgE antibodies against Ole e I, detected by double-antibody radioimmunoassay. HLA-DRB and -DQB loci were analyzed by PCR-SSO and RFLP. Phenotypic frequencies were compared with those of 179 healthy unrelated individuals. Significant increases in the phenotypic frequencies of DR7 (pf = 67.5% vs 31.3% in the control population, pc = 0.0023) and DQ2 (pf = 90.0% vs 48.0%, pc = 0.0003) were found, indicating an association between DRB1*0701/2, DQB1*0201 alleles and the IgE antibody response to Ole e I. This is the first time that the HLA-DQ gene has been associated with a positive allergic response.

HLA-B73: an atypical HLA-B molecule carrying a Bw6-epitope motif variant and a B pocket identical to HLA-B27

HLA-B73, first described by Mayr and Kirnbauer (1981), is a poorly characterized allospecificity, serologically related to the B7-CREG. We polymerase chain reaction-amplified, cloned and sequenced the HLA-B alleles of the B-LCL LE023, established from a Spanish Caucasoid individual expressing HLA-B73. 5 refs., 2 figs.

Allergy to olive pollen : T-cell response from olive allergic patients is restricted by DR7-DQ2 antigens

Backgound We have recently described the association between the IgE antibody response to Ole e I (the major antigen from olive tree pollen) and the DR7‐DQ2 haplotype in a Spanish population.

Molecular cloning and polymerase chain reaction-sequence-specific oligonucleotide detection of the allele encoding the novel allospecificity HLA-Cw6.2 (Cw∗1502) in Spanish gypsies

A proposed novel allospecificity, HLA-Cw6.2, has been reported to be commonly found (approximately 25%) in Spanish Gypsies. Full-length cDNAs of the allele (Cw*1502) coding for this antigen have been cloned in this study. A simple PCR-SSO method for its detection has been standardized and a correlation with the serologic Cw6.2 phenotype has been established. This specificity has been also detected in the homozygous typing cell RML. Although the primary structures of Cw*1502 and Cw*0601 are not closely related, they share specific motifs that may account for their serologic cross-reactivity. Two novel HLA-C alleles (Cw*12022 and Cw*0602) are also reported.

Nucleotide sequence of HLA-B*2706.

This conference will be held in Toulouse, France, at the Universite Paul Sabatier on August 21-24, 1996. The main topics will include: MHC and non-MHC alloantigenic systems, genetic mapping, T and B cells (receptors, subsets differentiation), cytokines, antigen-presenting cells, transplantation (allo and xeno, solid organ, and bone marrow), autoimmunity, monoclonal antibodies, chimeric molecules, and transgenic rats. Further information may be obtained from Professor Philippe Druet, INSERM U28 Hopital de Purpan, Place du Docteur Baylac, 31059 Toulouse Cedex, France; Telephone: ++33-61-77 25 66. Telefax: ++33-6177 25 68.

Cw*1505: a novel HLA-C allele isolated from a B*7301 haplotype

The Cw*15 allelic family is one of the most numerous within the HLA-C locus, with four members described so far. In this report, the authors describe Cw*1505, a new allele of this group isolated from the B-LCL of Spanish Caucasoid origin LE023. Cw*1505 differs from Cw*1502 by two point changes, one at exon 1 (synonymous), and the other at codon 116 (Leu {yields} Phe). 7 refs., 1 fig.

Characterization of an HLA-DR15 DQ5 haplotype found in the Spanish caucasoid population

HLA class II typing by RFLP and PCR-SSOP has been performed on HLA-DR2-positive individuals as a part of a study on MHC in a Spanish Caucasoid population. The results of this study reveal that HLA-DR15 (DRB1*1501 DRB5*0101) and DQ5 (DQA1*0102 DQB1*0501/0502) are not uncommonly associated in such a population. Family segregation has been assessed and allogeneic reactivity against some classic DR2 haplotypes has been tested; a stimulatory capability of DQ6 antigen in this situation is shown. It is suggested that the reported association is not uncommon in European Caucasoids as well as in other populations and it should be considered in matching for transplantation and in DR2-associated diseases.