M.L. González Pereyra

Fungi and selected mycotoxins from pre- and postfermented corn silage

Aim:  To determine fungal genera, Aspergillus and Fusarium species and aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON), fumonisin B1 (FB1) contamination from pre‐ and postfermented corn silage produced in the most important region of Argentina where silage practice is developed.

Determination of mycobiota and mycotoxins in pig feed in central Argentina

Aims:  To evaluate the mycobiota and natural levels of aflatoxins, fumonisins and zearalenone present in compound feed and home‐corn grains intended for fattening pigs.

Mycobiota and mycotoxins in malted barley and brewer's spent grain from Argentinean breweries.

Aims:  To evaluate mycobiota and aflatoxins B1 (AFB1), B2 (AFB2), G1 (AFG1), G2 (AFG2) and fumonisin B1 (FB1) contamination in different malted barley types and brands and brewer’s grain collected from a major Argentinean brewery.

Genotoxicity and cytotoxicity evaluation of probiotic Saccharomyces cerevisiae RC016: a 60-day subchronic oral toxicity study in rats

To acquire data on the safety‐in‐use of the probiotic Saccharomyces cerevisiae RC016 and test its ability to reduce genotoxicity caused by dietary aflatoxins (AFs).

Aflatoxins and Saccharomyces cerevisiae: yeast modulates the intestinal effect of aflatoxins, while aflatoxin B1 influences yeast ultrastructure

The gastrointestinal tract (GIT) is the main site where absorption of food components takes place and the first system coming into contact with mycotoxins of dietary origin. The aim of this work was to study the effect of probiotic Saccharomyces cerevisiae RC016 on intestinal villi of rats exposed to aflatoxins for 60 days. Moreover, the effect of in vitro aflatoxin B1 (AFB1) exposure on yeast cell ultrastructure was evaluated. Six treatments were applied (n=6) to inbred male Wistar rats: (1) uncontaminated feed control (F); (2) yeast control; (3) F + 40 μg/kg AFB1 + 20 μg/kg aflatoxin G1 (AFG1); (4) F + 100 μg/kg AFB1 + 50 μg/kg AFG1; (5) F + 40 μg/kg AFB1 + 20 μg/kg AFG1 + daily oral dose 108 viable S. cerevisiae cells; and (6) F + 100 μg/kg AFB1 + 50 μg/kg AFG1 + daily oral dose 108 viable S. cerevisiae cells. Morphometric measurements (villus length and width, crypt depth, quantification of goblet cells) were assessed using image analysis. S. cerevisiae RC016 cells were exposed to 20 μg/ml of AFB1 in ...