M Lassegues
Centre national de la recherche scientifique
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Featured researches published by M Lassegues.
Journal of Invertebrate Pathology | 1989
M Lassegues; Philippe Roch; Pierre Valembois
Abstract Coelomic fluid of the earthworm, Eisenia fetida andrei , naturally inhibits the in vitro growth of the two worm pathogen bacteria, Aeromonas hydrophila (Gram-negative) and Bacillus megaterium (Gram-positive). LD 50 values (injected dose causing death of 50% of the worm population) indicated that E. fetida andrei is more susceptible to A. hydrophila (5 × 10 3 living bacteria) than to B. megaterium (5 × 10 5 ). One injection of LD 50 resulted in an increased humoral bacteriostatic activity peaking 4 days after injection. Such enhanced activity totally protected the worms against infestation with 200 to 2000 times the LD 50 dose and persisted for 4 days with A. hydrophila and 11 days with B. megaterium . Secondary response, different from the first one, was obtained on animals reinjected with the LD 50 dose at the time when normal bacteriostatic activity was recovered (respectively 15 and 22 days after the first injection) but not 15 days later.
Journal of Invertebrate Pathology | 1988
Pierre Valembois; Philippe Roch; M Lassegues
Abstract A coagulation of Eisenia fetida andrei coelomic fluid occurs spontaneously in vitro. It is due to the conversion of two plasma proteins of MW 40 and 45 kDa in an insoluble fiber clot. A serine protease activity is suspected triggering the coagulation process. A potent enhancement of coagulation is obtained in presence of freeze-dried mucus, normally secreted by epidermal cells. The presence of chloragosomes favors the development of the clot network. Possible roles of clotting in earthworm life conditions are discussed.
Journal of Invertebrate Pathology | 1989
M Lassegues; Philippe Roch; Pierre Valembois
Abstract Adult earthworms Eisenia fetida andrei were found to increase their naturally occurring humoral bacteriostatic activity as a response to bacteria injection. Only the two earthworm pathogenic bacteria we tested, Bacillus megaterium (Gram-positive) and Aeromonas hydrophila (Gramnegative), induced this effect. In contrast, injecting earthworms with nonpathogenic bacteria, such as Acinetobacter sp. and Escherichia coli, as well as with sheep and human O erythrocytes or saline and sterile bacteria culture medium, did not modify the normal activity. When induced, the activity did not discriminate between the two pathogenic bacteria.
Journal of Invertebrate Pathology | 1982
Pierre Valembois; Ph. Roch; M Lassegues; Pierrette Cassand
Journal of Invertebrate Pathology | 1989
M Lassegues; Ph. Roch; Pierre Valembois
Biochimica et Biophysica Acta | 1997
Alexandra Milochau; M Lassegues; Pierre Valembois
Journal of Invertebrate Pathology | 1992
F Hirigoyenberry; M Lassegues; Ph. Roch
Journal of Invertebrate Pathology | 1989
M Lassegues; Ph. Roch; Pierre Valembois
Developmental and Comparative Immunology | 1989
Ph. Roch; M Lassegues; Pierre Valembois
Developmental and Comparative Immunology | 1989
M Lassegues; Ph. Roch; Pierre Valembois