M. M. A. Hassan

Physicochemical Characteristics and Spectroscopy of the Volatile Oil of Thymus vulgaris Growing in Saudi Arabia

AbstractThe volatile oil of Thymus vulgaris, L. (Fam. Labiatae) growing in the Northern region of Saudi Arabia was prepared and its percentage was determined. Its specific gravity, refractive index, solubility and optical rotation have been determined. The chemical constituents of the oil have been identified using TLC and GLC-Mass spectrometry techniques. Thymol content of the oil was determined by two different methods. The data obtained were used to compare it with the Spanish and Moroccan thyme oils, and commercial value was considered.

Chemical Composition and Antimicrobial Activity of the Essential Oil of Chenopodium botrys Growing in Saudi Arabia

AbstractChenopodium botrys L. growing in Saudi Arabia is rich in essential oil (2% v/w). The GC/MS and spectroscopic data of the gross terpenoid constituents are presented. α and β-eudesmol were the major sesquiterpenes in the oil. The antimicrobial activity of the oil is also recorded.

Physico-Chemical Characteristics and Spectroscopy of Ducrosia isamaelis Oil

Abstract Abstract - The essential oil of Ducrosia ismaelis, Asch. was prepared and its physical (specific gravity, refractive index, and optical rotation) and chemical standards (ester percentage) were determined. The constituents of the oil were identified using TLC, IR, PMR and GLC/Mass spectrometry techniques. Myrecene, N-undecane, α-pinene, n-butylbenzene, γ-cadinene, 3, 5-dimethylstyrene, 5-methylindan, P-menthadiene, cymene, γ-terpinene, sabinene, decanol, and fenchone are the major constituents.

PMR Assay of Essential Oils. IV. Assay of Carvone in Caraway and Dill Oils

Abstract A new assay procedure has been developed for the quantitation of carvone as a pure compound and in essential oils using PMR technique. The average recovery of pure carvone in standard mixtures using benzophenone as an internal standard is 100.11 + 0.56% w/w. Using the same procedure for the determination of carvone contents in Caraway and Dill oils, mean values of 61.7 + 0.59% w/w and 63.3 + 0.26% w/w, respectively, are obtained. The results are comparable to those obtained using a reported method. Beside its simplicity and accuracy, the technique may provide a mean for detection of impurities in the oil.

PMR Assay of Natural Products in Pharmaceuticals: II Assay of Khellin and Simultaneous Detection and Determination of Visnagin

Abstract A PMR method for the quantitative determination of khellin in bulk drug, tablets and injectables is presented. The determination is based on the integration of the 5-and 8-methoxyprotons of khellin relative to that of the methylprotons of acetanilide. The method is rapid, accurate and precise, with standard deviations of ± 0.76% in synthetic mixtures and ± 0.94% in tablets and injectables respectively. It furnishes a specific means of identification of khellin as well of the simultaneous detection and determination of the less potent 8-demethoxy analoge, visnagin.

Spectrophotometric Quantitation of Ornidazole and its Formulations

Abstract Summary - A rapid, accurate, precise and reproducible 1H-NMR assay and a direct UV one of ornidazole-bulk drug and tablet dosage forms are presented. The recoveries (Mean and S. D.%) by the 1H-NMR method were within 1% of those by the UV-method. The 1H-NMR method is based on the integration of the 2-methyl protons of ornidazole relative to that of the methyl protons of acetanilide (internal standard). The UV-method is based on the conjugated chromophoric nitroimidazole nucleus of ornidazole molecule. Moreover, very satisfactory mean recoveries for the bulk drug, added to its formulations, have been established.

Application of PMR Spectrometry in Quantitative Analysis of Tiaprofenic Acid in Some Pharmaceutical Preparations

Abstract A simple, accurate and specific proton magnetic resonance (PMR) procedure was developed for the assay of tiaprofenic acid and its tablets. The method involves comparing the integral of the nice doublet signal of tiaprofenic acid (positioned at 1.60 δ) to that of the sharp singlet signal (positioned at 2. 1 δ) of acetanilide which is used as internal standard. The method reported in this study gives accurate and reproducible results; 100. 16 ± 0. 23% for authentic tiaprofenic acid and 100.00 ± 1. 05% for tiaprofenic acid tablets (SurgamR). In addition, the PMR spectrum obtained helps in confirming the identify and purity of the drug.

Determination of Naloxone Hydrochloride in Dosage form by High-Performance Liquid Chromatography

Abstract A new, sensitive and rapid method for the determination of naloxone hydrochloride as drug in dosage entity and form using HPLC has been developed. Authentic naloxone hydrochloride was used to establish a calibration curve. A linear relationship was obtained for concentrations ranging from 10 μg/ml to 50 μg/ml. The column used was C18, Micropak MCH-10 (monomeric) and the mobile phase was acetonitrile : 0.01 M KH2PO4 (70 : 30) at a flow rate of 2 ml/min. Retention time for naloxone hydrochloride was 3.3 minutes. The proposed method has been proved accurate and precise compared to other pharmacopoeia methods of assay for naloxone hydrochloride.

PMR Assay of β-Lactm Antibiotics 1: Assay Of Cephalosporins

Abstract A PMR method for quantitative determination of cephalosporins namely cephalotin, cephaloridine, cephradine, cefoxitin and cefotaxime in bulk drugs and pharmaceutical formulation are presented. The determination is based on the integration of the 6-H and/or 7-H of the β-lactam ring system relative to that of the nine protons of t-butanol. The method is rapid, accurate and precise, with an average standard deviations of ± 1.51 in standard mixtures and ± 1.15 in pharmaceutical formulation. The procedure furnishes a specific means of identification of each individual cephalosporin as well as detection of the commonly encountered impurites.

PMR Assay of Natural Products in Pharmaceuticals. IV Assay of Codeine and Simultaneous Detection of Morphine

Abstract A rapid, accurate and precise PMR method is presented for the quantitative determination of codeine and codeine phosphate as bulk drugs and in tablet dosage form. The determination is based on the integration of the C-3 methylprotons or the two aromatic protons of codeine or its salt relative to that of the nine protons of t-butanol. Standard deviations of ± 1.39, 0.27 and 0.65% were obtained for codeine, codeine phosphate bulk drug and codeine phosphate tablets respectively. The method furnishes a specific means of identification of codeine as well as of the simultaneous detection and possible determination of morphine; thus fulfilling one of the most important pharmacopeial requirements for the purity of codeine.