M. M. Vasiliev

Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds.

Non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents is weak due to natural pathogen or biofilm resistance. The purpose of this study was to test the individual susceptibility of pathogenic bacteria to non-thermal argon plasma and to measure the effectiveness of plasma treatments against bacteria in biofilms and on wound surfaces. Overall, Gram-negative bacteria were more susceptible to plasma treatment than Gram-positive bacteria. For the Gram-negative bacteria Pseudomonas aeruginosa, Burkholderia cenocepacia and Escherichia coli, there were no survivors among the initial 10(5) c.f.u. after a 5 min plasma treatment. The susceptibility of Gram-positive bacteria was species- and strain-specific. Streptococcus pyogenes was the most resistant with 17 % survival of the initial 10(5) c.f.u. after a 5 min plasma treatment. Staphylococcus aureus had a strain-dependent resistance with 0 and 10 % survival from 10(5) c.f.u. of the Sa 78 and ATCC 6538 strains, respectively. Staphylococcus epidermidis and Enterococcus faecium had medium resistance. Non-ionized argon gas was not bactericidal. Biofilms partly protected bacteria, with the efficiency of protection dependent on biofilm thickness. Bacteria in deeper biofilm layers survived better after the plasma treatment. A rat model of a superficial slash wound infected with P. aeruginosa and the plasma-sensitive Staphylococcus aureus strain Sa 78 was used to assess the efficiency of argon plasma treatment. A 10 min treatment significantly reduced bacterial loads on the wound surface. A 5-day course of daily plasma treatments eliminated P. aeruginosa from the plasma-treated animals 2 days earlier than from the control ones. A statistically significant increase in the rate of wound closure was observed in plasma-treated animals after the third day of the course. Wound healing in plasma-treated animals slowed down after the course had been completed. Overall, the results show considerable potential for non-thermal argon plasma in eliminating pathogenic bacteria from biofilms and wound surfaces.

Non-thermal argon plasma is bactericidal for the intracellular bacterial pathogen Chlamydia trachomatis

Non-thermal plasma (NTP) is a flow of partially ionized argon gas at an ambient macroscopic temperature and is microbicidal for bacteria, viruses and fungi. Viability of the Gram-negative obligate intracellular bacterial parasite Chlamydia trachomatis and its host cells was investigated after NTP treatment. NTP treatment of C. trachomatis extracellular elementary bodies (EBs) diminished the concentration of infectious bacteria by a factor of 9×10(4), as established by the parallel infection of murine fibroblast McCoy cells with treated and control EBs. NTP treatment of infected McCoy cells caused disruption of membrane-restricted vacuoles (inclusions), where C. trachomatis intracellular reticulate bodies (RBs) multiply, and a 2×10(6)-fold reduction in the concentration of infectious bacteria. When the samples were covered with magnesium fluoride glass to obstruct plasma particles and UV rays alone were applied, the bactericidal effect was reduced 1.4×10(1)-fold and 5×10(4)-fold for EBs and RBs, respectively. NTP treatment caused the viability of host McCoy cells to diminish by 19%. Therefore, the results obtained demonstrated that (i) both extracellular and intracellular forms of C. trachomatis are sensitive to NTP treatment; (ii) the reduction in concentration of infectious bacteria after NTP treatment of infected cells is superior to the reduction in viability of host cells; and (iii) the effect of NTP on intracellular bacteria does not depend on UV rays.

Dynamics of dust structures in a dc discharge under action of axial magnetic field

Results of experiments on the formation of dusty plasma structures in a stratified dc discharge in axial magnetic fields up to 2500 G are presented. The rotation of the rather small and planar dusty plasma structures has been observed. As the field increases, the inversion of the structure rotation occurs, and when it reaches 700 G, the displacement of dust particles from the axial region of the discharge to the periphery, along with the rotation continuation, is observed. The explanation of the features in the dynamical behavior of the dust particles in the discharge, in particular the rotation inversion, is proposed.

Large-scale vortices in dc glow discharge dusty plasmas

Formation of large-scale dust vortices in the striation of dc glow discharges was experimentally investigated. Dust clouds were formed by monodisperse MF particles with diameters ≈2 µm in the regions of stable striation. Vortices obtained were about 1 cm in size and contained ~104 particles. Structural (vortex configuration, dust particle flow filaments) and dynamic (rotation frequencies and velocity spectrum) characteristics of large-scale dust vortices were measured.

Clusters of charged diamagnetic particles levitating in nonuniform magnetic field

For confinement and investigation of strongly coupled systems of charged macroparticles, we propose to use a trap based on the known possibility of the levitation of diamagnetic bodies in a nonuniform steady-state magnetic field. An experimental setup is described, which is capable of forming and confining clusters of charge graphite particles sized 100 to 300 μm in magnetic field B~1 T and |∇B| ~ 10 T/cm. It is inferred that it will be possible to form stable 3D structures containing several thousands macroparticles under terrestrial conditions by using fields B>10 T, and under microgravity conditions by using fields B~0.1 T.

Solid-hexatic-liquid transition in a two-dimensional system of charged dust particles

Two-stage melting is observed experimentally in a confined monolayer of dust particles in plasma. The pair correlation and bond-angular correlation functions, the number of topological defects, and the pair potentials are measured and analyzed. The bond-orientational correlation functions show a clear solid-to-hexatic-to-fluid transition, in perfect agreement with the Berezinskii-Kosterlitz-Thouless transition theory.

Coulomb scatter of diamagnetic dust particles in a cusp magnetic trap under microgravity conditions

The effect of a dc electric field on strongly nonideal Coulomb systems consisting of a large number (~104) of charged diamagnetic dust particles in a cusp magnetic trap are carried out aboard the Russian segment of the International Space Station (ISS) within the Coulomb Crystal experiment. Graphite particles of 100–400 μm in size are used in the experiments. Coulomb scatter of a dust cluster and the formation of threadlike chains of dust particles are observed experimentally. The processes observed are simulated by the molecular dynamics (MD) method.

Phase transitions and dynamic entropy in small two-dimensional systems: Experiment and numerical simulation

The results of experimental and numerical analysis are presented for phase transitions in strongly nonequilibrium small systems of strongly interacting Brownian particles. The dynamic entropy method is applied to analysis of the state of these systems. Experiments are carried out with kinetic heating of the structures of micron-size particles in a laboratory rf discharge plasma. Three phase states of these small systems are observed: crystalline, liquid, and transient. The mechanism of phase transitions in cluster structures of strongly interacting particles is described.

Coulomb structures of charged macroparticles in static magnetic traps at cryogenic temperatures

Electrically charged (up to 107e) macroscopic superconducting particles with sizes in the micrometer range confined in a static magnetic trap in liquid nitrogen and in nitrogen vapor at temperatures of 77–91 K are observed experimentally. The macroparticles with sizes up to 60 μm levitate in a nonuniform static magnetic field B ~ 2500 G. The formation of strongly correlated structures comprising as many as ~103 particles is reported. The average particle distance in these structures amounts to 475 μm. The coupling parameter and the Lindemann parameter of these structures are estimated to be ~107 and ~0.03, respectively, which is characteristic of strongly correlated crystalline or glasslike structures.

Low Temperature Atmospheric Argon Plasma: Diagnostics and Medical Applications

This study was devoted to diagnostic of low temperature plasma produced by microwave generator and investigation of its bactericidal effect against bacteria in biofilms and within eukaryotic cells. The profile of gas temperature near the torch outlet was measured. The spectrum in a wide range of wavelengths was derived by the method of optical emission spec-troscopy. Probe measurements of the floating potential of plasma were car-ried out. The estimation and adaptation of parameters of plasma flow (tem-perature, velocity, ion number density) according to medico-technical requirements were produced. The model of immersed surface-associated biofilms formed by Gram-negative bacteria, Pseudomonas aeruginosa and Burkholderia cenocepacia, and Gram-positive bacteria, Staphylococcus aureus, was used to assess bactericidal effects of plasma treatment. Reduction in the concentration of live bacteria in biofilms treated with plasma for 5 min was demonstrated by measuring Live/Dead fluorescent labeling and using direct plating. The intracellular infection model with the pathogenic bacterium, Chlamydia trachomatis, was used to study the efficacy of microwave argon plasma against intracellular parasites. A 2 min plasma treatment of mouse cells infected with C. trachomatis reduced infectious bacteria by a factor of 2×106. Plasma treatment diminished the number of viable host cells by about 20%. When the samples were covered with MgF2 glass to obstruct active particles and UV alone was applied, the bactericidal effect was re-duced by 5×104 fold compared to the whole plasma.