M. N. Saprykina

Microscopic fungi in water of the Dnieper river

Species nature of microscopic fungi-micromycetes in various areas of the Dnieper river and its inflows have been investigated. It has been shown, that, the basic main species isolated from water of the Dnieper and its inflows are Aspergillus, Penicillium, Cladosporium, Alternaria, Candida, and others. It has been established that quantitative micromycetes composition depends, to a large extent, on depth of water sampling, temperature, and the presence of organic substances. The influence of bathing people on quantitative and species compositions of micromycetes has been revealed.

The decontamination effect of UV radiation with respect to micromycetes

Isolated were pathogenic micromycetes from tap drinking water among which the most typical were Aspergillus niger, Aspergillus flavus, Aspergillus ochraceus, Penicillum cyclopium, Penicillium expansum, Cladosporium cladosporioides. The article has studied inactivation of microscopic fungus by UV radiation. It has also established a series of sensitivity of microorganisms to the effect of ultraviolet. It has been shown that such sanitary-indicative test microorganisms as Escherichia coli, Bacillius subtilis, Candida albicans cannot be used for assessing the degree of water decontamination by UV radiation by the mycological criterion. The stability of micromycetes to UV radiation is an evidence of a necessity to use complex methods of water decontamination.

Inactivation of Candida albicans in the photo-fenton system

The paper has investigated photocatalytic decontamination of the culture of micromycetes Candida albicans in water media depending on the radiation dose (UV-A range), the pH of the medium, the concentration of H2O2, ions of iron (II), (III), and phosphate ions. We proposed the interpretation of the impact of the specified factors on the rate of the course of the process being investigated. We showed prospects of using the UV/H2O2 and photo-Fenton systems as applied to decontamination of water media from micromycetes Candida albicans.

Formation of viable noncultural state of Candida Albicans

The paper has investigated the influence of NaOCl on the cells of Candida albicas with the aim of finding viable noncultural state and also conditions of their rehabilitation. Microscopic research of the cells Candida albicans has been given in the indicated state colored with trypan blue.

Identification of Escherichia Coli in the viable nonculturable state under exposure to chlorine

The influence of NaOCl on Escherichia coli cells has been investigated for the purpose of detecting their viable nonculturable state. The optimal temperature of transition of cells from the nonculturable into culturable state was also determined.

Inactivation of Candida Albicans in the UV/TiO2/Fe3+ system

The article has investigated the process of photocatalytic water deactivation of the microscopic fungus Candida albicans. We have determined optimal values of pH, established the impact of the concentration of the catalyst in the solution on the degree of inactivation of the culture. We have investigated the impact of the UV/TiO2/Fe3+ on inactivation and reparation of the culture Candida albicans

A new design of the electrocoagulation apparatus for removal of micromycetes from water

A new design of the electrocoagulation apparatus consisting of an inert anode and cathode made of stainless steel was developed. The formation of a coagulant takes place due to the corrosion of ferrous plates located in the anode plane. The following optimum parameters for the operation of the apparatus were defined: current density—21 mA/cm2, current strength—60 mA, area of ferrous plates—3 cm2. The application of this apparatus makes possible to convert efficiently Fe2+ to Fe3+ allowing the user to reduce the consumption of coagulant and remove to the maximum extent contaminants including micromycetes from water.

Adhesion analysis of micromycetes on granular media

The efficiency of using granular media in the form of sand and mesoporous carbon for the purpose of extracting microscopic fungi from water has been estimated. The main factors of micromycetes adhesion on the surface of sorbents were established with due regard for the electrokinetic characteristics of fungi cells. The biomass distribution of microscopic fungi over the depth of the filtration medium was also shown.

Detection of Microorganisms in Nonculturable State in Chlorinated Water

The nonculturable state in sanitary demonstrative microorganisms Escherichia coli and Candida albicans caused by the chlorine disinfection of water has been studied. The technique developed for detecting such microorganisms is assumed as a basis of the State Standard of Ukraine “Quality of water. Detection of microorganisms in nonculturable state in water”.

Genetically Safe Drinking Water. Requirements and Methods of Its Quality Control

The article contains a substantiation of a necessity of a new standard for assessment of the quality of genetically safe drinking water designed for consumption by man. Requirements to the quality of such water include 76 indices and are presented by 10 individual groups; extended is the number of indices of drinking waters of the level of toxicity (biotesting in 7 test objects), normative references to the methods of their control.