M. Ortega

Characterisation of three Amerindian populations from Hidalgo State (Mexico) by 15 STR-PCR polymorphisms

The purpose of this study is to report allele frequency data of three ethnic Amerindian population samples: the Otomi (Hña-hñu) from eastern Sierra Madre and Ixmiquilpan valley and the Huasteco from La Huasteca. These groups were characterised by 15 STR-PCR polymorphisms (HumTH01, HumvWA, D18S51, HumTPOX, D19S433, D16S539, D13S317, D8S1179, D7S820, D5S818, HumFGA, CSF1PO, D2S1338, D3S1358 and D21S11). No significant deviations in observed allelic frequencies from Hardy-Weinberg equilibrium were found for all the studied systems. From the forensic point of view, the heterozygosity value, power of discrimination and the a priori chance of exclusion were calculated.

Genetic Diversity of 15 STRs in Choles From Northeast of Chiapas (Mexico)

Allele frequencies for 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D19S433, HUMVWA31A, HUMTPOX, D18S51, D3S1358, HUMTHO1, D13S317, D16S539, D2S1338, D5S818, and HUMFGA) were analyzed in a Mexican population: Choles from Chiapas State. The aim of the study was to obtain accurate allele frequencies data and other genetic parameters of forensic interest on the Amerindian ethnic groups from Mexico, using an automatic method and commercial amplification kit. The agreement with HWE (Hardy-Weinberg equilibrium) was confirmed for all loci (based on the X2-test).

The Fang population of Equatorial Guinea characterised by 15 STR-PCR polymorphisms

Allele frequencies for 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D19S433, HUMVWA31A, HUMTPOX, D18S51, D3S1358, HUMTHO1, D13S317, D16S539, D2S1338, D5S818 and HUMFGA) were analysed in the Fang population of Bioko Island, Equatorial Guinea. No deviation from Hardy-Weinberg equilibrium was found for all loci. Statistical parameters demonstrated the forensic utility of the analysed systems.

Polimorfismos genéticos como indicadores de la vulnerabilidad individual a la adicción al tabaco

BACKGROUND AND OBJECTIVES In line with genetic and pharmacological studies suggesting that neurotransmitter pathways play a role in nicotine dependence, research was conducted in connection with 4 genetic polymorphisms: OPRM1, TPH1, ADRA2A and HTR1B. This study compares the genotype and allele frequencies in 3 groups (non-smokers, former smokers and smokers) of unrelated individuals (n=490) from Catalonia (north east Spain) in order to find any relationship. MATERIAL AND METHODS All polymorphisms were genotyped in each population group and statistical analysis was performed. RESULTS Data obtained show that there is a relationship between sex, age and the TPH1 locus, indicating a trend towards a lower frequency of the AA genotype in former smokers for the TPH1 locus. CONCLUSIONS The results indicate that a role is played by the TPH1 polymorphism as an indicator of therapeutic failure in smoking cessation.

Huastecos Amerindian population (Mexico) characterised by 12 STR-PCR polymorphisms

The purpose of this study is to report allele frequency data of a Huastecos ethnic group population sample (n=89) from La Huasteca (Hidalgo, Mexico) for 12 STR-PCR polymorphisms (HumTH01, HumvWA, D18S51, HumTPOX, D19S433, D16S539, D13S317, D8S1179, D7S820, D5S818, HumFGA and D21S11). No significant deviations from Hardy-Weinberg expectation were found for all short-tandem repeats (STR). From forensic point of view, the heterozygosity value, power of discrimination and the a priori chance exclusion value were calculated. D 2003 Elsevier B.V. All rights reserved.

Hña hñu Amerindian population from La Sierra Otomı́-Tepehua (Mexico) characterised by 14 STR–PCR polymorphisms

Abstract The purpose of this study is to report allele frequency data of Hna hnu (Otomi) ethnic Amerindian group population sample from La Sierra Otomi-Tepehua (Estado de Hidalgo-Mexico) for 14 STR–PCR polymorphisms (HumTH01, HumPenta D, HumPenta E, HumvWA, D18S51, D16S539, D13S317, D8S1179, D7S820, D5S818, D3S1358, HumCSF1PO, HumFGA and D21S11).

DYS19, DYS385, DYS389 (I and II), DYS390, DYS391, DYS392, DYS393 Haplotypes in a Population Sample from Catalonia (North-East Spain)

Blood samples (n = 224) from healthy unrelated individuals (males) were collected. DNA was extracted by using the phenolchloroform-isoamyl alcohol method (1). Hot-start PCR triplex (Triplex I: DYS19 and DYS389 I/II; Triplex II: DYS390, DYS391 and DYS393) and singleplex (DYS392) amplifications were accomplished with fluorescein labelled primers by the method published by Gene et al. (2). The primers used were those described by Kayser et al. (3) and de Knijff et al. (4). DYS385 locus was analyzed following the method and primers described by EDNAP group (5). Genotypes were analyzed in denaturing 6% polyacrilamide gel electrophoresis, using a monochrome automated laser fluorescence sequencer. The alleles from all loci reported were typed according to the published nomenclatures and the ISFG guidelines for STR analyses. Our group have received the certify of excellence in the Y-STR Haplotyping Quality Assurance Exercise 2000–2001 (http:/www.ystr.org).

Otomi Amerindian population (Mexico) characterized by HUMVWA, HUMTH01, HUMTPOX, HUMCSF1PO, D3S1358, and D19S253 STR-PCR polymorphisms

Six STR (HumvWA, HumTH01, HumTPOX, HumCSF1PO, D3S1358 and D19S253) have been analyzed in an Otomi population sample from Mexico. No deviations from Hardy–Weinberg equilibrium were found for all systems studied. Significant differences in allele frequencies were found between the Aymara and Otomi populations for four systems (HumTH01, HumvWA, D3S1358 and D19S253). This suggest a certain degree of genetic differentiation between the two populations.

Haplotype frequencies of DYS19, DYS389 I/II, DYS390, DYS391, DYS393 and DYS385 STR loci in Barcelona (North-East Spain)

Abstract We report a Y-chromosome haplotype frequency database obtained from a Barcelona population study. Samples were typed according to the published nomenclature and the ISFG guidelines for short tandem repeat (STR) analyses.