M. R. Dhiman

Genetic analysis of important antioxidant compounds in cabbage (Brassiaca oleracea var. capitata L.)

ABSTRACT The paucity of research on development of antioxidant-rich hybrids in cabbage by utilizing combining ability estimates, gene action, and heterosis motivated us to undertake this study. We made 60 crosses between 5 cytoplasmic male-sterile lines and 12 male-fertile testers during the summer of 2015, as per the line × tester design. The seedlings of all the parents and 60 F1 crosses, along with three checks, were transplanted during the Rabi (winter) season of 2015–2016 and evaluated using a randomized complete-block design. Combining ability, gene action, and heterosis were determined for different antioxidant compounds. Experimental results revealed that the range of cupric ion reducing antioxidant capacity (CUPRAC) [parents = 1.26–7.33 and hybrids = 0.04–6.54 μmol trolox/g], ferric reducing ability of plasma (FRAP) [parents = 1.65–4.76 and hybrids = 0.16–4.67 μmol trolox/g], β-carotene (parents = 0.44–2.29 and hybrids = 0.04–1.89 μg/100 g), and chlorophyll-a (parents = 0.71–4.08 and hybrids = 0.19–3.08 mg/g f.w.) for hybrids was lower than that of the parents because of outbreeding depression. The parental lines 6A, 208A, 83-5-8, and Sel-5-83-6 were found to be good general combiners for most of the antioxidant compounds studied. Based on the mean performance, specific combining ability effects and heterosis, five hybrid combinations viz., 9A × KIRC-8 for CUPRAC and FRAP; 208A × C-122 for ascorbic acid; 6A × Chhaki-2 for total carotenoids and β-carotene; 831A × Chhaki-2 for chlorophyll-a; and 6A × 83-5-8 for chlorophyll-b and total chlorophyll content, were most promising. The ratio of general combining ability (GCA) and specific combining ability (SCA) variances, i.e., [2 σ2g/(2σ2g + σ2s)], which reflects the relative importance of GCA versus SCA, was less than unity for different antioxidant compounds, which implied that for these traits, nonadditive gene effects were more important than additive effects. The numerical values of range for contribution of lines × testers interaction for different traits (41.47–70.18%) were found to be higher than the individual contribution of lines (11.24–47.22%) and testers (8.31–21.76%). Hence, heterosis could be exploited for developing antioxidant-rich hybrid cabbage.

Biodiversity in Lilium : A Review

Lilium is a subbstantial genus administered throughout temperate and cooler regions of the Northern Hemisphere consisting approximately 110 species. The genus possess a great genetic diversity in many valuable horticultural traits which is manifested in flower colour, forms, shape, size, fragrance, resistance to diseases, and many physiological characteristics. Intensive agricultural practices, climate change and industrialization are having a straight impact on biodiversity. Comprehensive understanding of the species, including levels and form of genetic variation forms the basis for the successful management and safeguarding of populations of rare, endangered or threatened species. The biodiversity become important components of different ecosystems. Use of single new improved varieties of crops for large areas is a big threat for crop biodiversity. This review concentrates to provide species-level information on biodiversity in the genus lilium and their future use in breeding programs. We focus mainly on species used in breeding programme and grown mainly for cut flowers and pot production. For example; trumpet shaped Lilium species showed comparative better prospective for exploitation than other species. We also present a brief summary on research area that needs further development using biotechnological techniques like molecular assisted breeding, QTLs and GISH/FISH and chloroplast genomes for comparative and phylogenetic analyses.