Maan Hong Jung

Multimodal imaging using optical coherence tomography and endolaryngeal ultrasonography in a new rabbit VX2 laryngeal cancer model.

Optical coherence tomography (OCT) provides ultrahigh‐resolution imaging of tissues within a depth of a few millimeters, whereas ultrasonography provides good imaging further below the surface. We aimed to develop a minimally invasive rabbit model of VX2 laryngeal cancer, suitable for these two imaging modalities through a transoral approach. We also sought to study the utility of combined OCT and endolaryngeal ultrasonography (EUS) for evaluation of early and advanced laryngeal cancer, using this model.

Optical imaging of subacute airway remodeling and adipose stem cell engraftment after airway injury

Acquired airway injury is frequently caused by endotracheal intubations, long-term tracheostomies, trauma, airway burns, and some systemic diseases. An effective and less invasive technique for both the early assessment and the early interventional treatment of acquired airway stenosis is therefore needed. Optical coherence tomography (OCT) has been proposed to have unique potential for early monitoring from the proliferative epithelium to the cartilage in acute airway injury. Additionally, stem cell therapy using adipose stem cells is being investigated as an option for early interventional treatment in airway and lung injury. Over the past decade, it has become possible to monitor the level of injury using OCT and to track the engraftment of stem cells using stem cell imaging in regenerative tissue. The purpose of this study was to assess the engraftment of exogenous adipose stem cells in injured tracheal epithelium with fluorescent microscopy and to detect and monitor the degree of airway injury in the same tracheal epithelium with OCT. OCT detected thickening of both the epithelium and basement membrane after tracheal scraping. The engraftment of adipose stem cells was successfully detected by fluorescent staining in the regenerative epithelium of injured tracheas. OCT has the potential to be a high-resolution imaging modality capable of detecting airway injury in combination with stem cell imaging in the same tracheal mucosa.

In vivo Optical Coherence Tomography Imaging of the Mesothelium Using Developed Window Models

The mesothelium is an essential lining for maintaining the normal homeostasis of the closed body cavity and a central component of pathophysiologic processes. The mesothelium has been known as the end target for asbestos which induces asbestos-related lung diseases. Malignant mesothelioma (MM) is a rare and fatal neoplasm predominantly due to asbestos exposure. Adaptation of an advanced and reliable technology is necessary for early detection of MM because it is difficult to diagnose this disease in its early stages. Optical coherence tomography (OCT) provides cross-sectional images of micro-tissue structures with a resolution of

Ex vivo High-resolution Optical Coherence Tomography (OCT) Imaging of Pleural Reaction after Pleurodesis Using Talc

2-10{mu}m

Tracheal cartilage regeneration by progenitor cells derived from the perichondrium

that can image the mesothelium with a thickness of

Peak Expiratory Flow(PEF) Measured by Peak Flow Meter and Correlation Between PEF and Other Ventilatory Parameters in Healthy Children

{sim}100{mu}m

Drug-Resistant Pulmonary Tuberculosis in Kosin Medical Center

and, therefore, enable investigation of early development of MM. The mesothelium is typically located at the pleura and tunica vaginalis of the scrotum. In this study, we developed animal window models in the above two anatomical sites to visualize mesothelial layers within the mesothelium. OCT images at the two locations were also acquired.

Intrapleural Doxycycline and Bleomycin in the Management of Malignant Pleural Effusions:A Randomized Study

The pleura is known as an end target organ of exposure to toxicn environmental materials such as fine particulate matter and asbestos.n Moreover, long-term exposure to hazardous materials can eventually lead ton fatal lung disease such as diffuse pleural fibrosis or mesothelioma. Chestn computed tomography (CT) and ultrasound are gold standard imagingn modalities for detection of advanced pleural disease. However, an diagnostic tool for early detection of pleural reaction has not beenn developed yet due to difficulties in imaging ultra-fine structure of then pleura. Optical coherence tomography (OCT), which provides cross-sectionaln images of micro tissue structures at a resolution of 2-10 µm, cann image the mesothelium with a thickness of ~100 µm and thereforen enables investigation of the early pleural reaction. In this study, wen induced the early pleural reaction according to a time sequence aftern pleurodesis using talc, which has been widely used in the clinical field.n The pleural reaction in talc grouped according to the time sequence (1st,n 2nd, 4th weeks) showed a significant thickening (average thickness: 45n ± 7.5 µm, 80 ± 10.7 µm, 90 ± 12.5 µm), whilen the pleural reaction in sham and normal groups showed pleural change fromn normal to minimal thickening (average thickness: 16 ± 5.5 µm, 17n ± 4.5 µm, 15 ± 6.5 µm, and 12 ± 7.5 µm, 13n ± 2.5 µm, 12 ± 3.5 µm). The measurement of pleuraln reaction by pathologic examinations was well-matched with the measurementn by OCT images. This is the first study for measuring the thickness ofn pleural reactions using a biophotonic modality such as OCT. Our resultsn showed that OCT can be useful for evaluating the early pleuraln reaction.

Isolation of nontuberculous mycobacteria by DNA probe and clinical characteristics of patients with NTM pulmonary disease

The regenerative potential of adult tracheal cartilage is very limited which makes repair of this cartilage extremely challenging. However, the perichondrium has a large quantity of progenitor cells that differentiate directly into chondrocytes. Bone morphogenetic protein (BMP) stimulates chondrogenesis by inducing the differentiation of progenitor cells into chondrocytes which makes it a potential therapeutic agent for cartilage regeneration. In this present study, we investigated the mesenchymal stem cell characterization of progenitor cells from the perichondrial tissue of tracheal cartilage and the efficiency of the perichondrium for tracheal cartilage regeneration by evaluating the effect of BMP on cartilage regeneration in perichondrial progenitor cells. Progenitor cells were isolated from the fibrous perichodrial tissue of tracheal cartilage and cultured for phenotypic characterization of mesenchymal stem cells. Next, a 0.3–0.5 mm gap was made in the mid-ventral portion of the tracheal cartilages. In the control group (n=5), the resulting gap was left untreated (without perichondrial replacement). In the perichondrial group (n=15), the gap was replaced by fibrous perichondrial tissue. We euthanized all mice in each group (n=5) at 2, 4, and 20 weeks after the perichondrial replacement and examined the replacement site microscopically. The immunohistochemistry for BMP-2 was performed in progenitor cells from perichondrial tissue. The primary cultured cells from fibrous perichondrial tissue were positive for CD29, CD90, and CD105, but negative for CD45. The progenitor cells were capable of multipotent differentiation towards adipogenic and osteogenic lineages. The control group showed no regenerated cartilage 2 weeks after injury. The experimental group demonstrated proliferation of progenitor cells between both stumps at 2 weeks and regenerated hyaline cartilage was observed at 4 and 20 weeks. Regenerated cartilage was observed as separated islets between the ends of the host cartilage stumps. The gaps were nearly filled with regenerated cartilage at 20 weeks. BMP-2 was expressed in both fibrous and cabial perichondria chondrogenesis. The progenitor cells from perichondrial tissue had mesenchymal stem cell-like features and might play a crucial role in tracheal cartilage regeneration and repair. Expression of BMP-2 may induce formation of structural cartilage in a tracheal injury model.

A Case of Esophago-mediatinal Fistula Due to Esophageal Tuberculosis

Background : In diagnosis or monitor of the airway obstruction in bronchial asthma, the measurement of in the standard method because of its reproducibility and accuracy. But the measurement of peak expiratory flow(PEF) by peak flow meter is much simpler and easier than that of especially in children. Yet there have been still no data of the predicted normal values of PEF measured by peak flow meter in Korean children. This study was conducted to provide equations to predict the normal value of PEF and correlation between PEF and in healthy children. Method : PEF was measured by MiniWright peak flow meter, and the forced expiratory volume and the maximum expiratory flow volume curves were measured by Microspiro HI 501(Chest Co.) in 346 healthy children(age:5-16 years, 194 boys and 152 girls) without any respiratory symptoms during 2 weeks before the study. The regression equations for various ventilatory parameters according to age and/or height, and the regression equations of by PEF were derived. Results : 1. The regression equation for PEF(L/min) was: age(year)+height(cm)-263() in boys, and age(year)+height(cm)-293() in girls. 2. The value of FEFmax(L/sec) derived from the maximum expiratory flow volume curves was multiplied by 60 to compare with PEF(L/min), and PEF was faster by 125 L/min in boys and 118 L/min in girls, respectively. 3. The regression equation for (ml) by PEF(L/min) was:PEF-550() in boys, and PEF-146 () in girls, respectively. Conclusion : This study provides regression equations predicting the normal values of PEF by age and/or height in children. And the equations for , a gold standard of ventilatory function, was predicted by PEF. So, in taking care of children with airway obstruction, PEF measured by the peak flow meter can provide useful information.