Magali Jouven

Circannual body reserve dynamics and metabolic profile changes in Romane ewes grazing on rangelands

Throughout an entire year, 41 Romane ewes reared in an extensive rangeland were used to investigate temporal changes in body reserves (BRs) and profiles of related metabolites and metabolic hormones. Ewes were allocated to homogeneous groups according to BW and BCS and were distributed by parity (primiparous [PRIM], n = 21; multiparous [MULT], n = 20) and litter size (LSi; lambing singletons [SING], n = 21 or TWINS, n = 20). The feeding system was based on rotational grazing of rangeland paddocks and progressive supplementation with hay, silage, and barley at late pregnancy during the winter. Individual BW, BCS, plasma NEFA, β-hydroxybutyrate (β-OHB), glucose, insulin, leptin, and triiodothyronine (T3) were monitored at -56, -12, 8, 49, 76, 107, 156, 195, 216, 246, and 301 d relative to lambing. The BR mobilization was observed from late pregnancy to the end of suckling and varied as a function of the ewe energy balance but also because of transitions from fertilized to native rangeland paddocks and by supplementation. Contrarily, BR accretion occurred from weaning, during the dry-off, and until the start of the next pregnancy. Lipolysis was well reflected by NEFA, β-OHB, and T3 kinetics. Mean BW (but not mean BCS) was affected by parity (MULT > PRIM), whereas both BW and BCS were influenced by LSi (SING > TWINS) but only for MULT. The most drastic BW loss was observed during the mid-suckling period (49 d in milk [DIM]) in all ewes. The lack of effects of LSi in PRIM but not in MULT was also evident in the majority of blood plasma kinetics, which were affected (P < 0.0001) by physiological stage in all ewes. A tendency to ketosis (β-OHB) was found in ewes nursing TWINS around lambing, irrespective of parity. Glucose concentrations were greater during suckling and dry-off, and a peak (0.96 ± 0.05 g/L) was attained at 156 DIM in MULT nursing TWINS. The highest plasma leptin concentration was observed during the start and the middle of the next pregnancy in MULT (107-216 DIM; 9.6 ± 0.44 ng/mL). In all ewes the physiological stage affected T3, which was affected by LSi just in MULT (from late pregnancy, MULT by SING > MULT by TWINS; 99.91 vs 85.52 ng/dL) and during suckling (111.7 ± 4.18 ng/dL). Lamb BW was affected at birth and weaning by parity (MULT > PRIM) and LSi (SING > TWINS). Overall, temporal changes in BR were directly affected by the transition of physiological states and feeding levels, whereas individual responses were predetermined by parity. In MULT, the reactivity and magnitude of response was influenced by LSi. The whole set of parameters allowed us to detect sensitive and critical periods throughout the entire annual cycle. We thus identified opportunities for improved nutritional management, for example, during physiological states usually underestimated such as early and mid-pregnancy. This work indicates the applicability of long-term studies about BR dynamics in ruminants as a potential component contributing to farm economic resilience.

Can virtual fences be used to control grazing sheep

Virtual fences (VF) are based on sensory cues delivered to domestic animals with the aim of controlling their spatial behaviour without any physical barrier. VF represent an approach for controlling the distribution of free-ranging large herbivores in large rangeland areas where the implementation of human control or the use of physical fencing is made impossible by their cost, environmental concerns or specific regulations. The potential of using VF for sheep was investigated by studying the spatial behaviour of groups of 5 or 32 ewes submitted to a commercial animal-borne VF device aimed at containing them in a defined area in a series of experiments. All tests were performed in pasture alleys virtually divided in their centre by a 4-m-wide warning/punishment zone). The ewes were introduced on one side of the zone, the other side being made attractive by the presence of tall grass (feed attractant), the presence of peers (social attractant) or being made unattractive by the presence of short grass (same feed as in the alley). In a first experiment, ewes were trained in groups of 5 with a feed attractant, following a 3-stage procedure: 30-min adaptation, 90-min training with a visual cue (three white strings above the wire of the VF) and 30-min test without a visual cue. In a second experiment, the test stage was repeated for each group with feed, social and zero attractants. In a third experiment, the test with feed attractant was performed with groups of 32 ewes comprising previously trained and equipped ewes mixed in different percentages of trained: naive (untrained and unequipped) ewes (100 : 0, 75 : 25, 50 : 50 and 0 : 100%). One year later, this test was repeated with a feed attractant either close (30 m) or far (60 m) from the punishment zone. By increasing the distance to the feed attractant, it was anticipated that this would stimulate the conflict of motivation between feed and peers. Ewes learned readily the concept of VF after a couple of contacts with the punishment zone. In the second experiment in the test with a social attractant, a few trained ewes penetrated the VF to re-associate with peers located in the pen opposite the VF. Expression of the behaviour to challenge or cross the punishment zone differed widely among individuals. In mixed groups, naive ewes crossed the punishment zone and lead part of their trained peers across the punishment zone, regardless of the distance of the feed attractant from the punishment zone. The results suggest that VF can be used to alter the distribution of grazing sheep within large fenced areas although they cannot replace conventional fences for absolute control.