Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Magda M. Aly is active.

Publication


Featured researches published by Magda M. Aly.


International Journal of Biological Macromolecules | 2013

Production and characterization of thermostable metallo-keratinase from newly isolated Bacillus subtilis NRC 3

Sanaa Tork; Yasser E. Shahein; Amr E. El-Hakim; Azza M. Abdel-Aty; Magda M. Aly

Novel keratinolytic enzyme (32kDa) secreted by a newly isolated Bacillus strain (Bacillus subtilis NRC3) cultivated in medium containing chicken feather meal was purified and partially characterized in a set of biochemical assays. The purification was carried out by applying a protocol of two successive chromatographic steps; cation exchange chromatography on CM-cellulose and gel filtration on sephadex G-75 columns. The purified enzyme showed a specific activity of 5233units/mg protein against 169units/mg protein for crude extract with 31 fold purification. The enzymatic activity of the purified keratinolytic enzyme stimulated by Na(+), K(+), Mg(2+), Ba(2+), Ca(2+), and inhibited by entire tested cations and metalloproteinase inhibitors, indicating that it belongs to metallo-keratinase enzymes. The optimum pH and temperature for the purified enzyme were (7.5, 8.0) and (50, 40°C) when using keratin azure and azocasein as substrates, respectively. The purified enzyme was highly stable at broad pH and temperature ranged (5-10) and (20-60°C), respectively. These results suggest that this keratinase may be a useful alternative and ecofriendly route for handling the abundant amount of waste feathers.


Food and Chemical Toxicology | 2010

Mineral content and microbiological examination of some white cheese in Jeddah, Saudi Arabia during summer 2008

Magda M. Aly; Madeha N. Al-Seeni; Safaa Y. Qusti; Nagwa M. El-Sawi

Different local and exported white cheese samples were collected from different markets in Jeddah during September 2008. Trace and heavy metals including Pb, Zn, Mn, Cu, Fe and Cd were analyzed using atomic absorption spectrometry. The concentration of the tested metals was in the range, Fe(++)>Zn(+++)>Mn(++)>Pb(++)>Cu(++)>Cd(++). The mean concentration of 7.63, 7.19, 0.5, 0.47, 0.16 and 0.14 μg/g was recorded for Fe, Zn, Mn, Pb, Cu and Cd, respectively. The concentration of iron ranged from 3.5 to 11.9 μg/g, zinc from 3.4 to 10.5, manganese from 0.12 to 1.0, lead from 0.14 to 1.14, and copper from 0.09 to 0.22. Yeasts and fungi were counted on Sabouraud and Potato Dextrose media and incubation was carried out at 25°C for 7 and 5 days, respectively. Yeast count and fungi count of cheese were ranged from 0.1 to 0.44CFU/g and from 0.123 to 1.11 CFU/g, respectively. Three out of 20 samples of cheese were contaminated with toxigenic fungi with 5% contamination level. Aflatoxin G1 was recorded in three samples using immunoadsorbent column chromatography with a range from 7 to 13 ppm.


International Journal of Biological Macromolecules | 2016

Purification and partial characterization of serine-metallokeratinase from a newly isolated Bacillus pumilus NRC21

Sanaa Tork; Yasser E. Shahein; Amr E. El-Hakim; Azza M. Abdel-Aty; Magda M. Aly

A serine metallokeratinase enzyme (30 kDa) produced by a newly isolated Bacillus strain (Bacillus pumilus NRC21) cultivated under optimized conditions in medium containing chicken feather meal was purified and characterized in a set of biochemical assays. The purification was carried out using two successive chromatographic steps; cation exchange chromatography on CM-cellulose and gel filtration on sephadex G-100 columns. The purified enzyme showed a specific activity of 2000 units/mg protein against 170 units/mg protein for crude extract with 12 fold purification. The enzymatic activity of the keratinase stimulated by (Na(+), K(+), Mg(2+)), Hg(+2) had no effect, and inhibited by entire tested cations, serine and metalloproteinase inhibitors, therefore it can be considered as a serine metalloenzyme. The optimum pH and temperature for the purified enzyme were (7.5, 8.5) and (50, 45 °C) when using keratin azure and azocasein as substrates, respectively. The purified enzyme was highly stable at broad pH and temperature ranged (5-10) and (20-60 °C), respectively and its thermoactivity and thermostability were enhanced in the presence of 5 mM Mg(+2). These results suggest that the purified keratinase may be used in several industrial applications.


Zeitschrift für Naturforschung C | 2015

Two new polyacetylene derivatives from the Red Sea sponge Xestospongia sp.

Seif-Eldin N. Ayyad; Dina F. Katoua; Walied M. Alarif; Tariq R. Sobahi; Magda M. Aly; Lamiaa A. Shaala; Mohamed A. Ghandourah

Abstract Two new polyacetylenes (1 and 2), along with two known C-30 steroids (3 and 4) were identified from the Red Sea sponge, Xestospongia sp. The chemical structures were determined based on extensive spectroscopic measurements 1D (1H, 13C and DEPT) and 2D (COSY, HSQC and HMBC) NMR, UV, IR and MS. The new compounds 1 and 2 were evaluated for their antimicrobial and antitumor activities. 1 and 2 were active against multidrug- resistant bacteria with MICs ranged from 2.2 to 4.5 μM. No toxicity was recorded for the two tested compounds up to 5 μM using Artemia salina as a test organism. Compound 2 showed excellent antifungal activity against some pathogenic fungi such as Aspergillus niger and Candida albicans (MIC 2.2–2.5 μM) and antitumor activity against both Ehrlich ascites carcinoma and lymphocytic leukemia (LD50 5.0 μM).


International Journal of Biological Macromolecules | 2015

Purification and characterization of gamma poly glutamic acid from newly Bacillus licheniformis NRC20.

Sanaa Tork; Magda M. Aly; Saleha Y. M. Alakilli; Madeha N. Al-Seeni

γ-poly glutamic acid (γ-PGA) has received considerable attention for pharmaceutical and biomedical applications. γ-PGA from the newly isolate Bacillus licheniformis NRC20 was purified and characterized using diffusion distance agar plate, mass spectrometry and thin layer chromatography. All analysis indicated that γ-PGA is a homopolymer composed of glutamic acid. Its molecular weight was determined to be 1266 kDa. It was composed of L- and D-glutamic acid residues. An amplicon of 3050 represents the γ-PGA-coding genes was obtained, sequenced and submitted in genbank database. Its amino acid sequence showed high similarity with that obtained from B. licheniformis strains. The bacterium NRC 20 was independent of L-glutamic acid but the polymer production enhanced when cultivated in medium containing L-glutamic acid as the sole nitrogen source. Finally we can conclude that γ-PGA production from B. licheniformis NRC20 has many promised applications in medicine, industry and nanotechnology.


International Journal of Biological Macromolecules | 2018

A new l -glutaminase from Streptomyces pratensis NRC 10: Gene identification, enzyme purification, and characterization

Sanaa Tork; Magda M. Aly; Omar Elsemin

In the current study, the purified l-glutaminase from Streptomyces pratensis NRC10 (GenBank number KC857622) was characterized. Its molecular weight was estimated to be 46kDa and isoelectric point 7.4. Its Vmax was calculated to be 2.19U/mg/min, while Km was 0.175mM. The optimum pH and temperature were 9 and 45°C, respectively. It was thermostable at 45°C but thermally inactivated at 60°C after 50min. Moreover, its enzymatic activity was enhanced by K+ ions and inhibited by Mg2+, Cu2+, Ag+, Hg2+, Ni2+, Fe2+, Cr2, Na+, Ca2+, and EDTA. A PCR fragment of 1550bp of S. pratensis NRC10 l-glutaminase gene (glsA) was purified and its sequence was determined (GenBank number KJ567136). l-glutaminase from NRC10 was induced mainly by l-glutamic acid. Model 3-D structure was composed of two domains, the serine - dependent beta-lactamase dominant the small STAS domain (Sulphate Transporter and anti-sigma factor antagonist) which had probably functioned as a general NTP binding domain. The two domains are linked by a linker peptide (GLHLMRNPALPGST), but sequence alignment between salt-tolerant glutaminase and the obtained glutaminase showed 44.75% of identity and 57% of similarity. This enzyme appears to have a distinctive structure compared to the rest of glutaminase family, and seems to construct a new subgroup of glutaminase.


Zeitschrift für Naturforschung C | 2017

Isolation, antimicrobial and antitumor activities of a new polyhydroxysteroid and a new diterpenoid from the soft coral Xenia umbellata.

Seif-Eldin N. Ayyad; Walied M. Alarif; Khalid Alfooty; Elham A. Selim; Mohamed A. Ghandourah; Magda M. Aly; Hajer S. Alorfi

Abstract A new C-30 steroid, 3β-,5α-,6β-,11α-,20β-pentahydroxygorgosterol (1), and a new diterpenoid, xeniumbellal (2), along with three known aromadendrane-type sesquiterpenes, aromadendrene (3), palustrol (4) and viridiflorol (5), were isolated from the soft coral Xenia umbellata. Chemical structures were determined by analyzing their NMR and MS data. The antimicrobial and antitumor activities of the isolated compounds were examined. Both 1 and 2 showed moderate antibacterial activities, especially against the multidrug-resistant Acinetobacter baumannii (MIC 0.22 and 0.28 mM, respectively); while 2 showed antitumor activity against a lymphoma cell line with LD50 0.57 mM and was nontoxic to Artemia salina at all tested concentrations up to about 4 mM.


Journal of Experimental Biology and Agricultural Sciences | 2017

PRODUCTION OF BIOFUEL FROM SUGARCANE BAGASSE WASTES USING Saccharomyces cerevisiae

Wafa A Baz; Lubna S Nawar; Magda M. Aly

The dry pulpy fibrous residue that remains after crush of sugarcane stalks is called Bagasse. It is Agroindustrial solid wastes which accumulated each day, causing big environmental problems. Saccharomyces cerevisiae is a unicellular fungus and has an interesting role in bioethanol production. In this study, two isolates of S. cerevisiae were used for bioethanol production from bagasse. In this study, Sugar cane bagasse were collected, hydrolyzed with concentrated HCl and used as a main carbon source (30 g/l). The growth curves of the two tested strains of S. cerevisiae were the same. The effect of temperature and pH levels on the growth, carbohydrates yields, and mainly bioethanol productivity from sugarcane wastes was studied for both Saccharomyces strains. The best conditions for bioethanol productivity was in fermentation medium after 3 days of growth at pH 6 and 30°C. In conclusion, Saccharomyces can be used for bioethanol production with the lowest possible costs from environmental wastes. * Corresponding author KEYWORDS


African Journal of Microbiology Research | 2012

Production of lipase from genetically improved Streptomyces exfoliates LP10 isolated from oil-contaminated soil

Magda M. Aly; Sanaa Tork; Saleh M. Al-Garni; Lubna S Nawar


Medicinal Chemistry Research | 2015

Rare pyrane-based cembranoids from the Red Sea soft coral Sarcophyton trocheliophorum as potential antimicrobial- antitumor agents

Khalid Alfooty; Walied M. Alarif; Fatma Asiri; Magda M. Aly; Seif-Eldin N. Ayyad

Collaboration


Dive into the Magda M. Aly's collaboration.

Top Co-Authors

Avatar

Sanaa Tork

King Abdulaziz University

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Khalid Alfooty

King Abdulaziz University

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Amr E. El-Hakim

King Abdulaziz University

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Bandar A. Babgi

King Abdulaziz University

View shared research outputs
Top Co-Authors

Avatar

Fatma Asiri

King Abdulaziz University

View shared research outputs
Researchain Logo
Decentralizing Knowledge