Magdi G. Shehata

Collection, storage and use of blood samples for future research: views of Egyptian patients expressed in a cross-sectional survey

Objective To determine the attitudes of Egyptian patients regarding their participation in research and with the collection, storage and future use of blood samples for research purposes. Design Cross-sectional survey. Study population Adult Egyptian patients (n=600) at rural and urban hospitals and clinics. Results Less than half of the study population (44.3%) felt that informed consent forms should provide research participants the option to have their blood samples stored for future research. Of these participants, 39.9% thought that consent forms should include the option that future research be restricted to the illness being studied. A slight majority (66.2%) would donate their samples for future genetic research. Respondents were more favourable towards having their blood samples exported to other Arab countries (62.0%) compared with countries in Europe (41.8%, p<0.001) and to the USA (37.2%, p<0.001). Conclusions This study shows that many individuals do not favour the donation of a blood sample for future research. Of those who do approve of such future research, many favour a consent model that includes an option restricting the future research to the illness being studied. Also, many Egyptians were hesitant to have their blood samples donated for genetic research or exported out of the Arab region to the USA and European countries. Further qualitative research should be performed to determine the underlying reasons for many of our results.

Dot-ELISA, a potential immunoassay for the detection of Plasmodium falciparum antibodies

The dot enzyme-linked immunosorbent assay (dot-ELISA) was compared to the indirect immunofluorescent assay (IIFA) and radioimmunoassay (RIA) for the detection of malaria antibodies. Of 281 sera tested by dot-ELISA, 220 were from Ethiopia, 11 from the Sudan, and 50 from Egypt. A close correlation between the dot-ELISA and RIA results was observed in 92% of the 220 Ethiopian cases. Of the remainder, 6% gave positive RIA and negative dot-ELISA results, and 2% gave positive dot-ELISA and negative RIA results. Antibody titres determined by dot-ELISA and RIA were positively correlated in 10 of the 11 Sudanese cases tested by direct microscopical examination. The eleventh case was positive by dot-ELISA at 1:1000 dilution, but negative by RIA and direct examination. With the 50 Egyptian sera, the dot-ELISA results showed close correspondence to the IIFA results, but the dot-ELISA was 20-40 fold more sensitive than the IIFA. To test specificity, 62 samples from patients with 11 different diseases and conditions were examined by dot-ELISA. No malaria antibodies were detected in any of these or in sera from healthy controls. Dot-ELISA is a potentially useful method for sero-epidemiological studies of malaria.

Morphometrics and protein profiles of the salivary glands of Phlebotomus papatasi and Phlebotomus langeroni sand flies

Sand fly salivary fluid contains numerous proteins that modulate host immune responses to infection and facilitate blood-feeding and establishing Leishmania infection. Salivary proteins are differentially expressed in adaptation to the host, the meal type and ecological factors. We report on the morphometrics and protein composition of salivary glands of colonised Phlebotomus papatasi and P. langeroni sand flies from Egypt. Female glands were dissected at day 1 (D1, unfed), day 2 (D2, sugar-fed), day 3 (D3, blood-fed) and day 7 (D7, blood-digested). The salivary glands are composed of two lobes: heterogeneous in P. papatasi and homogeneous in P. langeroni. Lobe sizes varied considerably with fly age and feeding state; D3 flies had the largest lobe sizes and protein content. The P. papatasi flies had larger lobes and higher protein content than the P. langeroni flies. The P. papatasi D1 flies had 15 protein bands that decreased in the D2, D3 and D7 flies to 10 bands in the Sinai flies and 9 bands in the Alexandria flies. All P. langeroni flies had 12 protein bands but with different intensities. The results reveal inter-specific variation between P. papatasi and P. langeroni, while no intra-specific variation between P. papatasi strains. These results increase our understanding of salivary gland protein composition and blood-feeding behaviour in Old World sand flies with implications for leishmaniasis epidemiology and control.

Development of a Local Entomological Database for Education and Research using Simulation (Virtual) Methods

Bioinformatics has been regarded as one of the rapidly-evolving fields with enormous impact on the history of life and biomedical sciences. It is an interdisciplinary science that integrates life sciences, mathematics and computer science in order to extract meaningful biological insights from large data sets of raw DNA and protein sequences. Our objective was the development of an entomogenomics database (provisionally named EntomDB) for education and research in entomology (entomology is the science of insects). This DB includes DNA/protein sequence data selected from genomes of major insect models of importance in biology and biomedical research. EntomDB will represent a customized easy, interactive and self- learning resource tool for beginner users in poor-resource settings. This will enable the users to learn basic skills in bioinformatics and genomics, needlessly to search through the numerous databases currently available on the World-Wide Web with their complex interfaces and contents. EntomDB will help students and young researchers in studying the primary structure, splicing, and translation and predict function of different genes by using simple simulation methods. It is also designed to be adaptable to work off-line, in case no internet connection is available. EntomDB is primarily designed for entomology discipline; however, it can easily be adapted for other disciplines in life and biomedical sciences. EntomDB will have important educational and developmental outcomes in promoting bioinformatics learning in the developing world and provide affordable first-level training for advanced degree and research levels.