Makbule Bilge Akbulut

Effect of Various Endodontic Irrigants on the Push-out Bond Strength of Biodentine and Conventional Root Perforation Repair Materials

INTRODUCTION The aim of this study was to evaluate the effect of various endodontic irrigants on the push-out bond strength of Biodentine (Septodont, Saint Maur des Fossés, France) in comparison with contemporary root perforation repair materials. METHODS Midroot dentin of canine teeth was horizontally sectioned into 1-mm-thick slices. The canal space of each dentin slice was enlarged with a diamond bur to 1.4 mm in diameter. The samples were divided into 5 groups (n = 40), and the following materials were placed, respectively: Biodentine, ProRoot MTA (Dentsply Tulsa Dental, Tulsa, OK), amalgam, Dyract AP (Dentsply DeTrey, Konstanz, Germany), and intermediate restorative material (IRM, Dentsply DeTrey). The samples were wrapped in wet gauze for 10 minutes and divided into 3 subgroups (n = 10) to be immersed into 3.5% sodium hypochlorite, 2% chlorhexidine gluconate (CHX), or saline for 30 minutes. No irrigation was performed in the controls (n = 10), and a wet cotton pellet was placed over each test material. After incubation for 48 hours, the dislodgement resistance of the samples was measured using a universal testing machine. The samples were examined under a stereomicroscope to determine the nature of the bond failures. RESULTS Biodentine showed significantly higher push-out bond strength than MTA (P < .05). The statistical ranking of push-out bond strength values was as follows: Dyract AP > amalgam ≥ IRM ≥ Biodentine > MTA. The push-out bond strength of Dyract AP, amalgam, IRM, and Biodentine was not significantly different when immersed in NaOCl, CHX, and saline solutions, whereas MTA lost strength when exposed to CHX. CONCLUSIONS Biodentine showed considerable performance as a perforation repair material even after being exposed to various endodontic irrigants, whereas MTA had the lowest push-out bond strength to root dentin.

Efficacy of Twisted File Adaptive, Reciproc and ProTaper Universal Retreatment instruments for root-canal-filling removal: A cone-beam computed tomography study

The aim of this study was to evaluate the efficacy of Twisted File (TF) Adaptive, Reciproc, and ProTaper Universal Retreatment (UR) System instruments for removing root-canal-filling. Sixty single rooted teeth were decoronated, instrumented and obturated. Preoperative CBCT scans were taken and the teeth were retreated with TF Adaptive, Reciproc, ProTaper UR, or hand files (n=15). Then, the teeth were rescanned, and the percentage volume of the residual root-canal-filling material was established. The total time for retreatment was recorded, and the data was statistically analyzed. The statistical ranking of the residual filling material volume was as follows: hand file=TF Adaptive>ProTaper UR=Reciproc. The ProTaper UR and Reciproc systems required shorter periods of time for retreatment. Root canal filling was more efficiently removed by using Reciproc and ProTaper UR instruments than TF Adaptive instruments and hand files. The TF Adaptive system was advantageous over hand files with regard to operating time.

Are dentin posts biomechanically intensive?: A laboratory and FEA study

The goal of this laboratory and finite elemental stress analysis (FEA) study was to compare fracture strength of dentin post (DP) with stainless steel and glass fiber posts. Single-rooted teeth were decoronated and restored with ParaPost (PP), i-TFC (TFC), and DPs (n = 10). Resin cores were created and loaded until failure (1.0 mm/min). The data were analyzed (ANOVA, Tukey, Chi-Square tests). Three-dimensional FEA models of the posts were created and the stress distributions were calculated using Solid Works/Cosmos works structural analysis program. A significant difference was found among the groups (p < 0.001). TFC group showed the highest and the DP group showed the lowest fracture resistance (p < 0.001). PP created more stress at the root dentin and inside the post. Restoration of roots with glass fiber and DPs may reduce stresses within the core material and the remaining root. However, a fracture-resistant restoration cannot be achieved by using a DP.

Wettability of AH Plus on root dentin treated with irrigation solutions with conventional methods or PIPS activation

Dr. Öğr. Üyesi Makbule Bilge Akbulut Karaciğan Mah. Ankara Cad. No: 74/a Karatay/Konya-Türkiye Tel: +90 544 440 75 25 E-posta: dt.bilge@yahoo.com ÖZET Amaç: Bu in vitro çalışmanın amacı; epoksi rezin içerikli kök kanal patının (AH Plus) sodyum hipoklorit (NaOCl), klorheksidin glukonat (CHX), etilendiamintetraasetik asit (EDTA) yıkama solüsyonlarının geleneksel yöntemle ya da foton indüklü fotoakustik dalgalanma (PIPS) aktivasyon tekniğiyle uygulandığı dentin yüzeyini ıslatabilirliğini değerlendirmektir. Gereç ve Yöntem: Bu çalışma için 60 adet tek köklü diş hazırlandı ve dişler silikon ölçü maddesi içerisine sabitlendi. Silikon sertleştikten sonra dişler bukkolingual yönde dikey olarak iki parçaya ayrıldı. Bir yarım diş örneği tekrar silikon içerisine yerleştirildi. Örnekler 6 deney grubuna ayrıldı (n=10) ve şu yıkama prosedürleri uygulandı: Grup 1, NaOCl; Grup 2, CHX; Grup 3, EDTA; Grup 4, NaOCl+PIPS; Grup 5, CHX+PIPS; Grup 6, EDTA+PIPS. İşlem görmeyen diğer yarım diş örneklerinden rastgele seçilen 10 tanesi kontrol grubu olarak kullanıldı ve distile su içerisinde bekletildi. Bir damla kök kanal patı her bir kuru dentin yüzeyine damlatıldı. Kanal patı ve kök dentini arasındaki temas açısı dinamik temas açısı ölçüm cihazıyla belirlendi. Veriler, tek yönlü varyans analizi ile istatistiksel olarak analiz edildi. Bulgular: Ortalama temas açısı değerleri NaOCl grubunda 60.93°, CHX grubunda 57.77°, EDTA grubunda 64,61° olup deney grupları ve kontrol grubunun temas açıları arasında istatistiksel olarak anlamlı fark bulunmamıştır (p>0,05). Yıkama solüsyonlarının PIPS ile aktivasyonu AH Plus’ın ıslatma davranışını değiştirmemiştir (p >0,05). Sonuç: Bu in vitro çalışmanın sınırları dahilinde yıkama solüsyonunun türü ve uygulama metodu AH Plus’ın ıslatma davranışını etkilememiştir. Anahtar kelimeler: AH Plus, ıslatabilirlik, kök kanal yıkama solüsyonları, PIPS, temas açısı

Effects of four novel root-end filling materials on the viability of periodontal ligament fibroblasts

Objectives The aim of this in vitro study was to evaluate the biocompatibility of newly proposed root-end filling materials, Biodentine, Micro-Mega mineral trioxide aggregate (MM-MTA), polymethylmethacrylate (PMMA) bone cement, and Smart Dentin Replacement (SDR), in comparison with contemporary root-end filling materials, intermediate restorative material (IRM), Dyract compomer, ProRoot MTA (PMTA), and Vitrebond, using human periodontal ligament (hPDL) fibroblasts. Materials and Methods Ten discs from each material were fabricated in sterile Teflon molds and 24-hour eluates were obtained from each root-end filling material in cell culture media after 1- or 3-day setting. hPDL fibroblasts were plated at a density of 5 × 103/well, and were incubated for 24 hours with 1:1, 1:2, 1:4, and 1:8 dilutions of eluates. Cell viability was evaluated by XTT assay. Data was statistically analysed. Apoptotic/necrotic activity of PDL cells exposed to material eluates was established by flow cytometry. Results The Vitrebond and IRM were significantly more cytotoxic than the other root-end filling materials (p < 0.05). Those cells exposed to the Biodentine and Dyract compomer eluates showed the highest survival rates (p < 0.05), while the PMTA, MM-MTA, SDR, and PMMA groups exhibited similar cell viabilities. Three-day samples were more cytotoxic than 1-day samples (p < 0.05). Eluates from the cements at 1:1 dilution were significantly more cytotoxic (p < 0.05). Vitrebond induced cell necrosis as indicated by flow cytometry. Conclusions This in vitro study demonstrated that Biodentine and Compomer were more biocompatible than the other root-end filling materials. Vitrebond eluate caused necrotic cell death.

Çeşitli Antiseptik Taşıyıcılarla Karıştırılan Kalsiyum Hidroksitin Enterococcus faecalis üzerindeki Antimikrobiyal Etkinliği

Amac: Bu in vitro calismanin amaci farkli antiseptik tasiyici - kalsiyum hidroksit (KH) kombinasyonlarinin Enterococcus faecalis (E. faecalis) ile enfekte kok kanallarindaki antibakteriyel etkinligini dentin blok modeli kullanarak test etmektir. Gerec ve Yontemler: Altmis adet cekilmis insan disi ornekleri hazirlandi. Elli-bes tane kok segmenti E. faecalis ile 2 hafta boyunca enfekte edildi. Ornekler 5 deney grubuna ayrildi (n=10) ve su KH – antiseptik tasiyici karisimlari uygulandi: Grup 1, KH + gliserin - distile su (7:1); Grup 2, KH + Octenisept; Grup 3, KH + %2’lik klorheksidin glukonat (CHX); Grup 4, KH + Savlex; Grup 5, KH + %5.25’lik sodyum hipoklorit (NaOCl). Antiseptik-KH karisimlari 7 gun boyunca kok kanallarinda bekletildi. Bes ornek sterilligi kontrol etmek icin (negatif kontrol), bes ornek de pozitif kontrol olarak kullanildi. Gates glidden frezler (# 3, 4, 5) yardimiyla kanal duvarlarindan elde edilen dentin ornekleri fosfat-tamponlu salin (PBS) ve cam boncuk iceren siselere aktarildi ve 30 sn vortekslendi. Bakteri iceren PBS’ye seri dilusyon yapildi. Her bir dilusyondan 25 μL halinde damlalar tripton soya agar plaklari uzerine ekildi ve 37°C’de 48 saat inkube edildi. Gorunur koloni sayilari incelendi ve log10 degerlerine donusturuldu. Istatistiksel analiz icin Kruskal-Wallis ve Mann Whitney-U testleri kullanildi. Bulgular: Negatif kontrol grubunda bakteri uremesi gorulmezken pozitif kontrol grubunun butun orneklerinde bakteri uremesi gozlemlenmistir (log10 3,47 CFU ml-1). Deney gruplari arasinda istatistiksel olarak anlamli bir fark bulunmamistir (p>0.05). KH + CHX ve KH + Savlex gruplarinda E. faecalis biyofilmi tamamen elimine edilmistir. Sonuc: Savlex, Octenisept ve NaOCl gibi cesitli antiseptik ajanlar KH ile karistirilarak kullanildiginda kok kanallarindan E. faecalis’i elimine etmede etkili bulunmustur.

Tooth discoloration effects of calcium silicate based barrier materials used in revascularization and treatment with internal bleaching

Background/purpose Usage of barrier materials is an important step in revascularization procedure. One of the undesired properties of these barrier materials is to cause coronal tooth discoloration. The aim of this in vitro study was to evaluate the tooth discoloration induced by ProRoot MTA (PMTA), Biodentine, and MM-MTA, as well as the efficacy of internal bleaching on this discoloration. Materials and methods Forty-two maxillary incisor teeth were prepared. Triple antibiotic paste (TAP) was placed in the root canals and incubated for 3 weeks. After removing the TAP, blood embedded spongostans were inserted into the root canals, and PMTA, Biodentine, or MM-MTA was placed over them. The teeth were incubated for 4 weeks at 37 °C; then, the internal bleaching agent was sealed for one week. The tooth color was measured throughout the study and the color change values (ΔE) of each specimen were calculated, and the data was statistically analyzed using the one-way ANOVA and Tamhanes T2 tests. Results The TAP significantly decreased the luminosity of the teeth (p < 0.05); however, no significant differences were observed between the tooth discolorations induced by the PMTA, Biodentine, and MM-MTA (p > 0.05). The teeth in the Biodentine group were more whitened than those of the PMTA and MM-MTA groups (p < 0.05). Conclusion Although the PMTA, Biodentine, and MM-MTA caused similar color alterations in the teeth, more bleaching was observed on those teeth discolored using TAP + blood + Biodentine.

Effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with Bioaggregate apical plugs

Abstract To evaluate the effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with DiaRoot BioAggregate (BA) when used as apical plugs. Forty single-rooted teeth were prepared using Peeso reamers. The samples were divided into four groups. The intracanal medicaments were applied to the root canals as follows: Group1: a combination of metronidazole–ciprofloxacin–cefaclor, Group2: a combination of metronidazole–ciprofloxacin, Group3: calcium hydroxide, and Group4: no medication. After 21 days, the medicaments were removed. The apical part of each root was horizontally sectioned into 1-mm thick slices. The samples were divided into two subgroups, and the following materials were placed: Biodentine, DiaRoot-BioAggregate. After 48-h incubation, the push-out bond strength was measured. The data were analyzed by a two-way ANOVA. Biodentine showed a significantly higher mean push-out bond strength value than DiaRoot-BioAggregate (P = 0.00). The medications have an effect on the push-out bond strength of both materials (P = 0.002). Biodentine showed better adhesive performance as an apical plug than DiaRoot-BioAggregate.