Maki Onoda

Analysis of urinary calculi obtained from a patient with idiopathic hypouricemia using micro area x-ray diffractometry and LC-MS

Urolithiasis is a common complication in patients with hypouricemia. Using a microarea x-ray diffractometer and nanoflow liquid chromatography-mass spectrometry (LC-MS) following SDS-polyacrylamide gel electrophoresis (PAGE), recurrent urinary calculi complicating a hypouricemic patient were analyzed. Analysis with the microarea x-ray diffractometer showed that one of the calculi was composed of calcium oxalate monohydrate and hydroxyapatite. The other was found to be formed from calcium oxalate dihydrate. After determination with LC-MS, both were found to contain uromodulin, albumin, osteopontin, protein Z, and defensins. Lysozyme and calgranulin A were also identified in these calculi. Defensins, which were antimicrobial peptides, and lysozyme, a mucopeptide glycohydrolase, were identified as new organic components of urinary stones. The role of these proteins in the process of urolithiasis is of particular interest.

Synthesis and Fluorescence Properties of 4,5-, 4,6- and 5,6-Disubstituted benzofurazan (2,1,3-Benzoxadiazole) Compounds

New 4,5-, 4,6- and 5,6-disubstituted benzofurazan (2,1,3-benzoxadiazole) compounds having various substituent groups were synthesized. Their fluorescence properties were obtained and the discussions were made to develop new sensitive and selective fluorescent reagents.

Fluorescence enhancement by hydroperoxides based on a change in the intramolecular charge transfer character of benzofurazan

Strong fluorescence signals were observed after the reaction of novel reagents with hydroperoxides.

Detection of prothrombin and osteopontin in a renal stone found in a hyperuricemic patient using 2D-PAGE and LC-MS analysis.

The liquid chromatography‐mass spectrometry (LC‐MS) following on from the two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE) technique was applied for the analysis of proteins in a renal stone found in a hyperuricemic patient. This technique was sensitive enough to detect small quantities of proteins even in a renal stone.