Makoto Washizu

Changes in glucose transport activities in mammary adenocarcinoma of dogs.

The activities of D-glucose transport (D-GT) and cytosolic enzymes were significantly higher in mammary adenocarcinoma of dogs than in mammary gland from normal dogs. The activities of D-GT in adenocarcinoma were over three-and-a-half times higher than in the controls. The K(m) value of the D-GT activity for glucose in both the adenocarcinoma and normal mammary gland was approximately 0.9 mM. The activities of the key glycolytic enzymes, hexokinase and pyruvate kinase, in the adenocarcinoma were also more than three-and-a-half times higher than in the controls. The increased activities of D-GT are considered to be accompanied by an acceleration of glucose utilisation in the adenocarcinoma of dogs.

Gallbladder lymphoma in a miniature dachshund.

A 7-year-old, miniature dachshund was referred for examination and treatment of persistent anorexia, deep yellow-coloured urine and leucocytosis. The clinical sign of jaundice, results from a serum biochemistry profile and ultrasonographic images suggested a biliary tract obstruction. A cholecystectomy was performed to remove the obstruction. Histopathological assessment of the resected gallbladder and partial common bile duct indicated diffuse large B-cell lymphoma. Twelve days after the initial operation, a second procedure was performed due to bile leakage into the abdominal cavity. Chemotherapy was administered twice after the second operation but discontinued, because the dog showed adverse effects. The dog is still alive 24 months after the surgery. To the authors’ knowledge, this is the first description of canine gallbladder lymphoma.

Incidence of ventricular premature contractions induced by thiopental sodium in dogs.

78頭の健康なビーグル犬を用い, チオペンタールナトリウム25mg/kgを静脈内投与した場合の心室性期外収縮の発生率を検討した結果, その発生率は30.8%であった。期外収縮の発生の見られた犬に再度チオペソタールを投与した場合その発生率は79.2%で高い再現性を示した。眼瞼反射の消失を指標としてチオペンタールナトリウムの投与量を決定した場合, 麻酔前処置薬を併用すると, 期外収縮の発生率が軽度に低下した。この効果は主にチオペンタールの投与量の減少によるものと考えられた。