Malcolm H. McGavran

Ultrastructural, cytochemical, and microchemical observations on cytomegalovirus (salivary gland virus) infection of human cells in tissue culture☆

Abstract The cytoplasmic body that forms, in addition to the nuclear inclusion, in cells infected with cytomegalovirus has been shown to be an aggregate of lysosomes. These lysosomes appear and surround viruses as they are released from the nucleus. The extent of inactivation of the virus remains to be determined. The outer of the two coats of CMV is derived from the inner nuclear membrane during the exodus of the virus from the nucleus.

Eosinophilic Granuloma of Bone: A Study of Twenty-eight Cases

1. Twenty-eight cases of eosinophilic granuloma of bone in children and adults, all but one with symptomatically solitary lesions, are reported. The skull, femur, ribs, and mandible were the most frequent sites of involvement. 2. No diagnostic roentgenographic characteristics were found. Lesions of the flat bones were generally punched-out osteolytic lesions. Lesions of long bones were easily confused with malignant tumors. Biopsy is considered imperative to establish the diagnosis. 3. Transition of eosinophilic granuloma to Hand-Schuller-Christian disease or Abt-Letterer-Siwe syndrome has not been encountered in these cases and we have some doubts whether the reported cases of transition represent true changes from one form to another. 4. Therapy has included excision, curettage, small doses of irradiation, or nothing. Evaluation of any one form of treatment is impossible. All the lesions reported in this study healed regardless of the therapy. 5. Meticulous microbiological studies of the tissue from these lesions seem indicated.

The ultrastructure of papillary cystadenoma lymphomatosum of the parotid gland

The ultrastructure of papillary cystadenoma lymphomatosum (PCL), Warthins tumor, of the parotid salivary gland has been described. The oncocytic cells are filled with mitochondria. Two morphologic forms of mitochondria are seen. The cells of the striate ducts in the normal parotid, have specializations associated with fluid transport and are structurally most nearly akin to the oxyphils. The subcellular hyperplasia of mitochondria may be a manifestation of aberrant function. Die onkocytÄren Zellen sind voll von Mitochondrien. Zwei Formen von Mitochondrien sind nachweisbar. Die Zellen der Streifenstücke (Speichelrohre) der normalen Parotis besitzen besondere Einrichtungen, die mit dem Flüssigkeitstransport in Zusammenhang gebracht werden, und sind gestaltlich am nÄchsten mit den oxyphilen Zellen verwandt. Die subcellulÄre Hyperplasie der Mitochondrien könnte Ausdruck einer abwegigen Funktion darstellen.

Chromaffin Cell: Electron Microscopic Identification in the Human Dermis

A granulated cell in human skin has an ultrastructure similar to that of chromaffin cells of the adrenal medulla. This cell is closely associated with unmyelinated axons and may be the site of peripheral store of noradrenaline.