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Dive into the research topics where Manfred Schmidt is active.

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Featured researches published by Manfred Schmidt.


FEBS Letters | 1996

HEAT-LABILE URACIL-DNA GLYCOSYLASE : PURIFICATION AND CHARACTERIZATION

Harald Sobek; Manfred Schmidt; B. Frey; Klaus Kaluza

A uracil‐DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23 400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat‐labile in dilute buffers (free of stabilizers) with a half‐life of 2 min at 40°C. Due to the thermolability, uracil‐DNA glycosylase is suitable for application in the carryover prevention technique showing less residual activity and/or a slower reactivation rate than the usually applied UNG from Escherichia coli.


Analytical Biochemistry | 1998

A Positive Selection Vector for Cloning of Long Polymerase Chain Reaction Fragments Based on a Lethal Mutant of thecrpGene ofEscherichia coli

Daniel Schlieper; Brigitte von Wilcken-Bergmann; Manfred Schmidt; Harald Sobek; Benno Müller-Hill


Archive | 2001

Method for producing an active heterodimeric AMV-RT in prokaryotic cells

Harald Sobek; Rainer Mueller; Manfred Schmidt; Bruno Frey; Bernhard Suppmann; Rainer Schmuck; Johann-Peter Thalhofer; Peter Pallua; Markus Pajatsch


Archive | 2003

Recombinant terminal deoxynucleotidyl transferase with improved functionality

Rainer Mueller; Markus Pajatsch; Ingo Curdt; Harald Sobek; Manfred Schmidt; Bernhard Suppmann; Kirsten Sonn; Bernd Schneidinger


Archive | 1997

Positive selection vector based on the caps gene, pCAPs vector and its use

Daniel Schlieper; Harlad Sobek; Manfred Schmidt; Brigitte von Wilcken-Bergmann; B Müller-Hill


Archive | 2011

Novel T7 RNA polymerase variants with enhanced thermostability

Michael Greif; Rainer Mueller; Christian Rudolph; Armin Ruf; Manfred Schmidt; Harald Sobek; Johann-Peter Thalhofer


Archive | 2010

Composition alpha-galactosidase aqueuse et procédés associés

Werner Hoelke; Rainer Mueller; Manfred Schmidt; Harald Sobek; Johann-Peter Thalhofer


Archive | 2002

Rekombinante terminale Deoxynukleotidyltransferase mit verbesserter Funktionalität Recombinant terminal deoxynucleotidyl transferase with improved functionality

Rainer Mueller; Markus Pajatsch; Ingo Curdt; Harald Sobek; Manfred Schmidt; Bernhard Suppmann; Kirsten Sonn; Bernd Schneidinger


Archive | 2001

Verfahren zur herstellung von aktive, heterodimere, amv-reverse transkriptase in prokaryonten

Bruno Frey; Rainer Mueller; Markus Pajatsch; Peter Pallua; Manfred Schmidt; Rainer Schmuck; Harald Sobek; Bernhard Suppmann; Johann-Peter Thalhofer


Archive | 2001

Verfahren zur herstellung von aktive, heterodimere, amv-reverse transkriptase in prokaryonten A process for producing active heterodimeric AMV-reverse transcriptase in procaryotes

Harald Sobek; Rainer Mueller; Manfred Schmidt; Bruno Frey; Bernhard Suppmann; Rainer Schmuck; Johann-Peter Thalhofer; Peter Pallua; Markus Pajatsch

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