Manish Bajpai

Noninvasive recording of electrocardiogram in conscious rat: A new device

Aim: Electrocardiogram (ECG) is an important tool for the study of cardiac electrophysiology both in human beings and experimental animals. Existing methods of ECG recording in small animals like rat have several limitations and ECG recordings of the anesthetized rat lack validity for heart rate (HR) variability analysis. The aim of the present study was to validate the ECG data from new device with ECG of anesthetized rat. Materials and Methods: The ECG was recorded on students physiograph (BioDevice, Ambala) and suitable coupler and electrodes in six animals first by the newly developed device in conscious state and second in anesthetized state (stabilized technique). Results: The data obtained were analyzed using unpaired t-test showed no significant difference (P < 0.05) in QTc, QRS, and HR recorded by new device and established device in rats. Conclusion: No previous study describes a similar ECG recording in conscious state of rats. Thus, the present method may be a most physiological and inexpensive alternative to other methods. In this study, the animals were not restrained; they were just secured and represent a potential strength of the study.