Manisha Srivastava

Effect of Temperature on Production of Endoglucanase and Xylanase from Some Green Manure Fungal Decomposers

Among the various microorganisms reported for their capabilities of cellulases and xylanases production, fungi are the most potent producers. This study reports the production of xylanolytic and cellulolytic enzymes by potential decomposing fungal isolates using a cheap medium containing green manure substrate and chemically defined basal medium under solid-state culture. A number of decomposing fungi were screened for cellulolytic and xylanolytic potential. The enzyme production with purified carboxy methyl cellulose (CMC) and birch wood xylan was studied and found to be promising. Aspergillus niger produced the highest amount of endoglucanase and xylanase viz., 3.635 U mg -1 protein min -1 and 2498.20 U mg -1 protein min -1 , respectively at 25°C. Growth and enzyme production was affected by the alterations in temperature. Highest production of endoglucanse and xylanase were noted at 25°C and 30°C.