Manojit Bhattacharya

MicroRNAs mediated regulation of MAPK signaling pathways in chronic myeloid leukemia

Chronic myeloid leukemia (CML) is a severe problem throughout the world and requires identification of novel targets for its treatment. This multifactorial disease accounts for about 15% of the all diagnosed leukemia cases. Mitogen-activated protein kinase (MAPK) signaling pathway is crucial for the cell survival and its dysregulation is being implicated in various types of cancers. In here, we have discussed the potential role of various miRNAs that are found involved in regulating the proteins cascades of MAPK signaling pathway associated with CML. An emphasis has been paid to summarize the influence of various miRNAs in elevating or suppressing the expression level of significant proteins such as miR-203, miR-196a, miR-196b, miR-30a, miR-29b, miR-138 in BCR-ABL tyrosine kinase; miR-126, miR-221, miR-128, miR-15a, miR-188-5p, miR-17 in CRK family proteins; miR-155, miR-181a with SOS proteins; miR-155, miR-19a, with KRAS proteins; miR-19a with RAF1 protein; and miR-17, miR-19a, miR-17-92 cluster with MAPK/ERK proteins. In light of ever-increasing importance and ever-widening regulatory roles of miRNAs in cells, we have reviewed the recent progress in the field of miRNAs and have tried to suggest them as controlling targets for various protein cascades of MAPK signaling pathway. An understanding of the supervisory mechanism of MAPK by miRNAs might provide novel targets for treating CML.

The crucial role and regulations of miRNAs in zebrafish development.

To comprehend the events during developmental biology, fundamental knowledge about the basic machinery of regulation is a prerequisite. MicroRNA (miRNAs) act as regulators in most of the biological processes and recently, it has been concluded that miRNAs can act as modulatory factors even during developmental process from lower to higher animal. Zebrafish, because of its favorable attributes like tiny size, transparent embryo, and rapid external embryonic development, has gained a preferable status among all other available experimental animal models. Currently, zebrafish is being utilized for experimental studies related to stem cells, regenerative molecular medicine as well drug discovery. Therefore, it is important to understand precisely about the various miRNAs that controls developmental biology of this vertebrate model. In here, we have discussed about the miRNA-controlled zebrafish developmental stages with a special emphasis on different miRNA families such as miR-430, miR-200, and miR-133. Moreover, we have also reviewed the role of various miRNAs during embryonic and vascular development stages of zebrafish. In addition, efforts have been made to summarize the involvement of miRNAs in the development of different body parts such as the brain, eye, heart, muscle, and fin, etc. In each section, we have tried to fulfill the gaps of zebrafish developmental biology with the help of available knowledge of miRNA research. We hope that precise knowledge about the miRNA-regulated developmental stages of zebrafish may further help the researchers to efficiently utilize this vertebrate model for experimental purpose.

Ichthyofaunal Diversity and Water Quality in the Kangsabati Reservoir, West Bengal, India

The ichthyofauna in relation to water quality was studied on monthly basis from March, 2010 to February, 2011 in the Kangsabati Reservoir, West Bengal. The study revealed that physicochemical parameters of Kangsabati Reservoir were congenial for 39 fish species of commercial importance, belonging to 7 orders, 15 families, and 26 genera. The Cypriniformes were dominant with 17 species, followed by Siluriformes and Perciformes, with 7 species each, Channiformes with 3 species, Osteoglossiformes and Synbranchiformes with 2 species each, and Anguilliformes with 1 species. Regarding their conservation status, 27 species were of least concern, 1 species was vulnerable, 6 species were near threatened, 1 species was data deficient, and 4 species were not evaluated (IUCN-Version 2014.1). Economical values have also been evaluated. Water parameters such as temperature, pH, alkalinity, dissolved oxygen, hardness, free CO2, salinity, total inorganic nitrogen, and phosphate were recorded and found suitable for fish production. Conductivity, transparency, and high chloride level are minor limiting factor that may needs rectification for improved fisheries management.

Freshwater fish resource mapping and conservation strategies of West Bengal, India

Explorations and Germplasm estimation of fish biodiversity in the freshwater bodies of India are being gradually updated and analysed with a number of new discoveries though a well-defined figure of existing freshwater fish species at regional and state level remains to be calculated. The freshwater resources of India are currently experiencing an alarming decline trend in term of piscine biodiversity due to several undesired factors and consequences a considerable portion of freshwater fishes have been categorized as Threatened category. West Bengal is now becomes one of the most significant states of freshwater fish producer in India. The research study workout and divide the West Bengal state into six major physiographic divisions along with the help of modern Geoinformatics techniques (ArcGIS 10.2, Google Earth and Global Mapper software for map making purpose). The present study deals with the freshwater fish resource, distribution and biodiversity assessment of entire part of West Bengal. Occurrence of 251 fish species belonging to 15 orders, 50 families have been noted during the study period of 2012–2016. It is evident that the members belonging to Cyprinifomes constitute 37% of the total fish fauna of the river. Fluctuation in occurrence and abundance of the species are influenced by several anthropogenic activities. The demand of proper management of conservation policy is highlights through our research work.

Threats to Fish and Fisheries of Kangsabati Reservoir, West Bengal, India

Fishes belonging to the Orders Beloniformes, Cypriniformes, Perciformes, Siluriformes and Synbranchiformes are usually found in the Kangsabati reservoir of West Bengal, India. In recent years among the trapped fishes fishermen failed to get certain fish species which were available to them in the last decade. This prompted us to conduct a survey of ichthyofauna of the said reservoir in respect to the water parameters, keeping in view the anthropogenic activity-induced pollution scenario. It is revealed that the fishes belonging to the species Xenentodon cancila, Nemacheilus savona, Sillaginopsis panijus, Pangasius sutchi, Colisa sota, Mystus cavasius, Mystus seenghala and Mastacembelus armatus are completely absent in the survey area. It is most likely that the eutrophication-induced causes especially, variations in composition and density of plankton as well as the undesirable changes in physical and chemical properties of the water have forced these fishes to migrate elsewhere.

Environmental DNA (eDNA): A Promising Biological Survey Tool for Aquatic Species Detection

Aquatic species are facing at higher risk of extinction similar to that of any other living components of diversified ecosystem in present scenario. So that, the conservation of aquatic biodiversity is much more important to know about the accurate information regarding species composition and their biological community interactions. Generally, traditional survey methods depend on physical identification and characterization of species but it has some sorts of challenging chances due to the phenotypic plasticity, sibling species, different stages of life cycle and its invasiveness. To overcome such barriers one of the significant and promising tool likewise environmental DNA (eDNA), which way the collection of genetic materials from bulk environment (i.e. soil, water, sediment etc.) circuitously from organisms has been used to monitor and analyzed the biodiversity status, invasive species along with the species of conservation category. Recently, the real application of eDNA analysis based outcomes uphold the actual emerging know how practices in support of the population and community ecology, conservation biology as well as in the superior field of taxonomical research. Such scientific appraisal will be useful in understanding the brief history of aquatic eDNA and obviously its methodological considerations, gentle sources, collection and analysis process, physical form, its persistence and proper transport in aquatic ecosystem. Moreover, the fruitful drives for summarization the discoveries of eDNA application and method over traditional technique, its recent challenges and examine the current and future frontiers along with the appropriate practices of aquatic eDNA relevancy in aquatic ecosystem.

Therapeutic applications of zebrafish (Danio rerio) miRNAs linked with human diseases: A prospective review

MicroRNAs (miRNAs) are the class of small, non-coding RNAs that are produced from precursor transcripts by subsequent processing steps mediated by members of the RNaseIII family, Dicer and Drosha protein within cell. The importance of zebrafish miRNAs in regulation of normal cellular development and support to various kinds of metabolism process. Although the zebrafish model provides a fundamental platform for the study of developmental biology but recent work with zebrafish model has expanded its appliance to a broad range of experimental studies relevant to different kind of human diseases. Presently, the zebrafish model is used for the study of cardiovascular disease, schizophrenia, bipolar I disorder in eyes, psoriasis, spinal cord injury, cancer and diabetes that showing in some selected miRNAs are regulate these diseases in molecular levels. Here, a superior drive performed to depict the fundamental utilization of the zebrafish miRNAs that targeted to several clinical diseases connected to human. This review aims to provide a summary of understanding of the cellular mechanism which is responsible for selected diseases and suggests some therapeutic application for inhibition of miRNA functions.