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Featured researches published by Maomin Lv.


Vox Sanguinis | 2012

Human parvovirus PARV4 in plasma pools of Chinese origin

Yuyuan Ma; Y. Guo; Xiong Zhao; Z. Wang; Maomin Lv; Q.-P. Yan; Jingang Zhang

Human parvovirus 4 (PARV4) is present in blood and blood products. As the presence and levels of PARV4 in Chinese source plasma pools have never been determined, we implemented real‐time quantitative PCR to investigate the presence of PARV4 in source plasma pools in China. Results showed that 26·15% (51/195) of lots tested positive for PARV4. The amounts of DNA ranged from 2·83 × 103 copies/ml to 2·35×107 copies/ml plasma. The high level of PARV4 in plasma pools may pose a potential risk to recipients. Further studies on the pathogenesis of PARV4 are urgently required.


Transplantation Proceedings | 2010

Real-Time Quantitative Polymerase Chain Reaction With SYBR Green I Detection for Estimating Copy Numbers of Porcine Endogenous Retrovirus From Chinese Miniature Pigs

Yuyuan Ma; Y. Yang; Maomin Lv; Q.-P. Yan; L. Zheng; F. Ding; Jianmin Wu; K. Tian; Jingang Zhang

Porcine endogenous retrovirus (PERV) in the pig genome represents a potential infectious risk in xenotransplantation. Chinese miniature pigs have been considered to be potential organ donors in China. However, an adequate level of information on PERV from Chinese miniature pigs has not been available. We established an SYBR Green I-based real-time quantitative polymerase chain reaction (PCR) assay for estimating copy numbers of PERV integrated in the host genome. The assay was 100-fold more sensitive compared with conventional PCR. We also evaluated the specificity and reproducibility of the assay. We statistically analyzed the difference in PERV copy numbers integrated into the genomes of Wuzhishan pigs versus Bama minipigs. This approach will be useful to screen donor pigs as well as to examine clinical samples from human subjects treated with porcine xenotransplantation products for evidence of PERV transmission.


Comparative Immunology Microbiology and Infectious Diseases | 2010

Identification of full-length proviral DNA of porcine endogenous retrovirus from Chinese Wuzhishan miniature pigs inbred

Yuyuan Ma; Maomin Lv; Shu Xu; Jianmin Wu; Kegong Tian; Jingang Zhang

Existence of porcine endogenous retrovirus (PERV) hinders pigs to be used in clinical xenotransplantation to alleviate the shortage of human transplants. Chinese miniature pigs are potential organ donors for xenotransplantation in China. However, so far, an adequate level of information on the molecular characteristics of PERV from Chinese miniature pigs has not been available. We described here the cloning and characterization of full-length proviral DNA of PERV from Chinese Wuzhishan miniature pigs inbred (WZSP). Full-length nucleotide sequences of PERV-WZSP and other PERVs were aligned and phylogenetic tree was constructed from deduced amino-acid sequences of env. The results demonstrated that the full-length proviral DNA of PERV-WZSP belongs to gammaretrovirus and shares high similarity with other PERVs. Sequence analysis also suggested that different patterns of LTR existed in the same porcine germ line and partial PERV-C sequence may recombine with PERV-A sequence in LTR.


Pharmaceutical Biology | 2016

Development of novel application of 3,3′-diindolylmethane: sensitizing multidrug resistance human breast cancer cells to γ-irradiation

Wenjing Wang; Maomin Lv; Yanlin Wang; Jingang Zhang

Abstract Context: Multidrug resistance (MDR) is known as a major obstacle to effective cancer therapy. The effects of irradiation on MDR in cancer cells had rarely been reported. Objective: The effect of 3,3′-diindolylmethane (DIM) sensitizing MDR human breast carcinoma to γ-irradiation was investigated. Materials and methods: MCF-7/ADR cells were exposed to different concentrations of DIM (0–30 μM) for 48 or 2 h before IR (γ-Co60, 10 Gy, room temperature) then cultured for 48 h. Cell survival was determined by MTT assay. Intracellular reactive oxygen spices (ROS) induced by DIM (20 and 30 μM, 2 h before irradiation) was measured by flow cytometry. Propidium iodide staining assay was used for cell cycle distribution studies; cell apoptosis was measured by flow cytometry and confocal microscopy. Results: DIM (20 and 30 μM, 2 h before irradiation) sensitized MCF-7/ADR cells to IR with survival rates decreased from 100% to 79% and 63%, respectively. DIM combined with γ-radiation demonstrated that the activity of G2/M phase cell cycle arresting with percentages enhanced from 9% to 49% and 52%. DIM can increase intracellular ROS generation by 1.45- and 1.55-times compared to control group. Significantly enhanced radiation-induced apoptosis by DIM was also observed. Discussion and conclusion: These data provide a rationale for the use of DIM as a promising radio-sensitizer to MDR cancer cells.


Journal of Chromatography B | 2016

Purification of α2-macroglobulin from Cohn Fraction IV by immobilized metal affinity chromatography: A promising method for the better utilization of plasma

Chaoji Huangfu; Yuyuan Ma; Maomin Lv; Junting Jia; Xiong Zhao; Jingang Zhang

As an abundant plasma protein, α2-macroglobulin (α2-M) participates widely in physiological and pathological activities including coagulation regulation, antitumor activities, and regulation of cytokines. It also presents a therapeutic potential for radiation injury. A two-step isolation method for the purification of α2-M from Cohn Fraction IV is described. This process includes a salting-out method and immobilized metal affinity chromatography. The LC-ESI-MS/MS analysis and a comparison of the amino acid composition demonstrated that the final product was α2-M. The final protein, with a purity of approximately 95% and a yield of nearly 45%, was obtained from Cohn Fraction IV regardless of plasma haptoglobin type, although all but type 1-1 have previously been considered unfavorable for α2-M preparation. The effects of temperature, pH, and methylamine on α2-M activity were evaluated to avoid activity loss during preparation and preservation. The results suggested that α2-M activity could be readily inactivated at temperatures above 50°C, at pH levels above 9.0 or below 4.0, or in the presence of methylamine. Cohn Fraction IV is usually discarded as a biological waste product in the human serum albumin production process; because the simple process developed in this study is relatively inexpensive, the preparation of α2-M from Cohn Fraction IV may better utilize human plasma, a valuable resource.


Journal of Immunotoxicology | 2014

Evaluation of a novel ultra-sensitive nanoparticle probe-based assay for ricin detection.

Huiqiong Yin; Min-xian Jia; Li-jun Shi; Jun Liu; Rui Wang; Maomin Lv; Yuyuan Ma; Xiong Zhao; Jingang Zhang

Abstract A gold nanoparticle (GNP) probe-based assay (GNPA) modified from the bio-barcode assay (BCA) was developed for ultrasensitive detection of ricin, a potential biothreat agent. In the GNPA, a chain of ricin was captured by a GNP probe coated with polyclonal antibodies and single-stranded signal DNA. A magnetic microparticle (MMP) probe coated with ricin A chain monoclonal antibody was then added to form an immuno-complex. After being magnetically separated, the immuno-complex containing the single-stranded signal DNA was characterized by PCR and real-time PCR. A detection limit of 10−2 fg/ml was determined for the ricin A chain; this is eight orders of magnitude more sensitive than that achieved with an ELISA and two orders more sensitive than that obtained with the BCA. The coefficients of variation (CV) of the intra- and inter-assay values ranged from 3.82–6.46%. The results here show that this novel assay is an ultrasensitive method for detection of ricin proteins and may be suitable for the ultrasensitive detection of other proteins.


Transfusion | 2016

Inactivation of viruses during a new manufacturing process of α2-macroglobulin from Cohn Fraction IV by dry-heat treatment.

Chaoji Huangfu; Xiong Zhao; Maomin Lv; Junting Jia; Fengxuan Zhu; Rui Wang; Yuyuan Ma; Jingang Zhang

α2‐Macroglobulin (α2‐M) has a curative effect on radiation injury. Virus transmission through plasma derivatives is still not risk‐free. Effect of dry heat on α2‐M activity and virus inactivation by dry heat in a new manufacturing process of α2‐M were studied.


Virology Journal | 2013

Construction and characterization of an infectious replication competent clone of porcine endogenous retrovirus from Chinese miniature pigs

Silong Xiang; Yuyuan Ma; Qipo Yan; Maomin Lv; Xiong Zhao; Huiqiong Yin; Nian Zhang; Junting Jia; Rong Yu; Jingang Zhang

BackgroundXenotransplantation from animals has been considered to be a preferable approach to alleviate the shortage of human allografts. Pigs are the most suitable candidate because of the anatomical and physiological similarities shared with humans as well as ethical concerns. However, it may be associated with the risk of transmission of infectious porcine pathogens. Porcine endogenous retroviruses (PERVs) are of particular concern because they have been shown to infect human cells in vitro. To date, researches on the molecular characteristics and potential pathogenicity of PERV are still tenuous. In this report, an infectious replication competent clone of PERV from Wuzhishan pigs (WZSPs) in China was generated and characterized. This infectious clone will contribute to studies on PERV virology and control of PERV in xenotransplantation using Chinese miniature pigs.MethodsThe proviral DNA of PERV from WZSPs was amplified in two overlapping halves. Then the two fragments were isolated, subcloned and fused to generate pBluescriptαSK+-WZS-PERV recombinant clones. Screened with RT-PCR, a molecular clone of PERV designated as WZS-PERV(2) was selected. Its infectivity and replication competency were characterized in HEK293 cells by PCR, real-time fluorescent quantitative RT-PCR, western blot, indirect immunofluorescence assay as well as sequence analysis.ResultsThe ability of WZS-PERV(2) to infect human cells and produce infectious virions were shown after transfection of the clone into HEK293 cells and infection of PERV derived from this recombinant clone. The expression of Gag proteins were detected in HEK293 cells infected with the virus derived from the clone by the indirect immunofluorescence assay and western blot. The results of sequences analysis and comparison combined with the PCR based genotyping result demonstrated that the WZS-PERV(2) belonged to PERV-A subgroup. Compared with a previous proviral DNA clone of PERV (PERV-WZSP), G to A hypermutation occurred in the env gene of WZS-PERV(2) was found, whereas APOBEC proteins have the potential to inhibit the replication of a variety of retroviruses through a cDNA cytosine deamination mechanism, so we presumed these G to A hypermutation might be the contribution of porcine APOBEC3F.ConclusionsAltogether, an infectious replication competent clone of PERV from Chinese miniature pigs (WZSPs) termed WZS-PERV(2) was generated, characterized and sequenced.


Journal of Automated Methods & Management in Chemistry | 2015

Developing a Novel Indolocarbazole as Histone Deacetylases Inhibitor against Leukemia Cell Lines

Wenjing Wang; Maomin Lv; Xiong Zhao; Jingang Zhang

A novel indolocarbazole (named as ZW2-1) possessing HDAC inhibition activity was synthesized and evaluated against human leukemia cell lines HL-60 and NB4. ZW2-1 performed anti-population growth effect which was in a concentration-dependent manner (2–12 μM) by inducing both apoptosis and autophagy in cells. The compound also caused differentiation of HL-60 and NB4 cells as shown by increasing expression of CD11b, CD14, and CD38 at moderate concentration (4 μM). At relatively high concentration (8 μM), ZW2-1 significantly decreased intracellular histone deacetylase 1 level which was also observed. All the results indicated that ZW2-1 could be a novel antileukemia lead capable of simultaneously inducing apoptosis, autophagy, and differentiation.


Biotechnology and Applied Biochemistry | 2018

Large‐scale purification of high purity α1‐antitrypsin from Cohn Fraction IV with virus inactivation by solvent/detergent and dry‐heat treatment

Chaoji Huangfu; Jinchao Zhang; Yuyuan Ma; Junting Jia; Jingxuan Li; Maomin Lv; Xiaowei Ma; Xiong Zhao; Jingang Zhang

α1‐Antitrypsin (AAT) is widely used to treat patients with congenital AAT deficiency. Cohn Fraction IV (Cohn F IV) is normally discarded during the manufacturing process of albumin but contains approximately 33% of plasma AAT. We established a new process for large‐scale purification of AAT from it. liquid chromatography‐electrospray ionization‐tandem mass spectrometry and high‐performance liquid chromatography were applied for qualitative identification and composition analysis, respectively. Stabilizers were optimized for AAT activity protection during lyophilization and dry‐heat. Virus inactivation by dry‐heat and solvent/detergent (S/D) was validated on a range of viruses. AAT with purity of 95.54%, specific activity of 3,938.5 IU/mg, and yield of 26.79%, was achieved. More than 95% activity was reserved after S/D. More than 96% activity was obtained after lyophilization or dry‐heat. After S/D, pseudorabies virus (PRV) and vesicular stomatitis virus (VSV) were inactivated below detectable level within 1 H. Virus titer reductions of more than 5.50 log10 and 5.38 log10 were achieved for PRV and VSV, respectively. Porcine parvovirus and encephalomyocarditis virus were inactivated by 3.17 log10 and 5.88 log10 reduction after dry‐heat. The advantages of this process, including suitability for large‐scale production, high purity, better utilization of human plasma, viral safety, commercial and inexpensive chromatography medium, may facilitate its further application.

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Jingang Zhang

Academy of Military Medical Sciences

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Yuyuan Ma

Academy of Military Medical Sciences

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Xiong Zhao

Academy of Military Medical Sciences

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Huiqiong Yin

Academy of Military Medical Sciences

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Jianmin Wu

Academy of Military Medical Sciences

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Wenjing Wang

Academy of Military Medical Sciences

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Q.-P. Yan

Academy of Military Medical Sciences

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Rui Wang

Academy of Military Medical Sciences

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F. Ding

Academy of Military Medical Sciences

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Jun Liu

Academy of Military Medical Sciences

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